Human Proinsulin,PI ELISA Kit
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中文名稱:人胰島素原(PI)酶聯(lián)免疫試劑盒
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貨號:CSB-E08345h
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規(guī)格:96T/48T
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價格:¥3200/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人胰島素原(PI)酶聯(lián)免疫試劑盒(CSB-E08345h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿,組織勻漿樣本中的PI含量。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL,適用于探索2型糖尿病發(fā)病機制、胰島移植效果評估、胰島素抵抗研究及藥物干預(yù)對β細(xì)胞功能的影響等科研場景。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:N/A
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縮寫:PI
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma,tissue homogenates
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檢測范圍:62.5 pg/mL-4000 pg/mL
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靈敏度:15.6 pg/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Signal Transduction
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human PI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
91
Range %
87-97
1:2
Average %
99
Range %
96-104
1:4
Average %
101
Range %
95-106
1:8
Average %
95
Range %
90-98
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回收率:
The recovery of human PI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
91
87-97
EDTA plasma (n=4)
99
93-105
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
4000
2.255
2.187
2.221
2.083
2000
1.638
1.732
1.685
1.547
1000
1.215
1.314
1.265
1.127
500
0.788
0.802
0.795
0.657
250
0.462
0.501
0.482
0.344
125
0.355
0.346
0.351
0.213
62.5
0.247
0.238
0.243
0.105
0
0.139
0.137
0.138
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Cytosolic pH is a direct nexus in linking environmental cues with insulin processing and secretion in pancreatic β cells Yujiang Fang,Cell Metabolism,2024
- Outcomes of metabolic surgery in obese patients with type 2 diabetes with respect to impact on beta cell function, insulin sensitivity and diabetes remission - A study from south India S Chandru,Diabetes & Metabolic Syndrome: Clinical Research & Reviews,2020
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