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  4. Human Prostaglandin D2(PGD2) ELISA kit

Human Prostaglandin D2(PGD2) ELISA kit

  • 中文名稱(chēng)：
    人前列腺素D2(PGD2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13898h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人前列腺素D2(PGD2)酶聯(lián)免疫試劑盒（CSB-E13898h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、組織培養(yǎng)上清液、組織勻漿、尿液樣本中的PGD2含量。PGD2即前列腺素D2，是一種重要的脂質(zhì)介質(zhì)。它參與多種生理病理過(guò)程，如炎癥反應(yīng)、睡眠調(diào)節(jié)等。在研究機(jī)制上，PGD2通過(guò)與特定受體結(jié)合，激活下游信號(hào)通路，調(diào)控細(xì)胞的功能和基因表達(dá)，是炎癥相關(guān)疾病研究的熱門(mén)靶點(diǎn)。試劑盒檢測(cè)范圍為23.5 pg/mL-1500 pg/mL，該產(chǎn)品可廣泛應(yīng)用于炎癥性疾病機(jī)制研究、藥物篩選模型中的PGD2動(dòng)態(tài)監(jiān)測(cè)、泌尿系統(tǒng)相關(guān)科研中的生物標(biāo)志物分析，以及代謝組學(xué)研究中的脂質(zhì)介質(zhì)定量檢測(cè)，為體外實(shí)驗(yàn)和動(dòng)物模型研究提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    N/A
  • 縮寫(xiě)：
    PGD2
  • 種屬：
    Homo sapiens (Human)
  • 樣本類(lèi)型：
    serum, cell culture supernates, tissue homogenates, urine
  • 檢測(cè)范圍：
    23.5 pg/mL-1500 pg/mL
  • 靈敏度：
    5.8 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PGD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %80-94
    1:2Average %98
    Range %91-105
    1:4Average %99
    Range %94-110
    1:8Average %94
    Range %87-98
  • 回收率：
    The recovery of human PGD2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9689-102
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15001.985 1.854 1.920 1.840
    7501.403 1.323 1.363 1.283
    3750.912 0.886 0.899 0.819
    187.50.545 0.525 0.535 0.455
    940.371 0.359 0.365 0.285
    470.204 0.198 0.201 0.121
    23.50.129 0.126 0.128 0.048
    00.080 0.079 0.080 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:

We have previously ordered these kits from you and have been running the project since 2014. I want to keep the same assays as before but I am wondering if you can provide any quality control material? In the past we didn’t have quality control material available. I understand that the kit comes with the required standards but it would be great if a positive and negative control could be purchased to be run with the kit as well. I would purchase these separately if available.Any recommendations as controls for this kit?

A:
Thanks for your inquiry.
CSB-E13898h
The standards in the kit can be used for quantitative analysis and control, so we didn't provide extra control.
Our kit is for research use only and has been optimized. Customers only need to do blank control during the assay and additional quality control is not needed. Pls let me know if you have any further questions. Thank you.