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  4. Human Protein FAM3C(FAM3C) ELISA kit

Human Protein FAM3C(FAM3C) ELISA kit

  • 中文名稱：
    人FAM3C蛋白(FAM3C)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL008228HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人FAM3C蛋白(FAM3C)酶聯(lián)免疫試劑盒（CSB-EL008228HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FAM3C含量。FAM3C，也稱為ILEI，是FAM3家族的一員，主要存在于分泌性上皮細(xì)胞中。它在視網(wǎng)膜層狀形成、上皮間質(zhì)轉(zhuǎn)化和胰腺癌發(fā)生中發(fā)揮重要作用。研究機制上，F(xiàn)AM3C可能通過調(diào)節(jié)細(xì)胞分化和組織重塑影響細(xì)胞行為，其具體作用機制尚在研究中。試劑盒檢測范圍為1.56 ng/ml- 100 ng/ml，適用于體外培養(yǎng)細(xì)胞模型、動物實驗組織樣本或人類體液樣本中FAM3C蛋白表達(dá)水平的動態(tài)監(jiān)測，為探索其在腫瘤微環(huán)境調(diào)控、代謝性疾病機制或藥物干預(yù)研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Fam3c ELISA Kit; FAM3C_HUMAN ELISA Kit; Family with sequence similarity 3 member C ELISA Kit; GS3786 ELISA Kit; ILEI ELISA Kit; Interleukin-like EMT inducer ELISA Kit; Predicted osteoblast protein ELISA Kit; Protein FAM3C ELISA Kit
  • 縮寫：
    FAM3C
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.56 ng/ml - 100 ng/ml
  • 靈敏度：
    0.39 ng/ml
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human FAM3C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    93

    Range %

    88-98

    1:2

    Average %

    96

    Range %

    92-101

    1:4

    Average %

    92

    Range %

    87-98

    1:8

    Average %

    97

    Range %

    94-101

  • 回收率：

    The recovery of human FAM3C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    98

    90-105

    EDTA plasma (n=4)

    93

    89-100

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    100

    1.941

    2.010

    1.976

    1.875

    50

    1.476

    1.570

    1.523

    1.422

    25

    1.073

    1.061

    1.067

    0.966

    12.5

    0.702

    0.751

    0.727

    0.626

    6.25

    0.404

    0.432

    0.418

    0.317

    3.12

    0.312

    0.322

    0.317

    0.216

    1.56

    0.201

    0.205

    0.203

    0.102

    0

    0.102

    0.099

    0.101

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    May be involved in retinal laminar formation. Promotes epithelial to mesenchymal transition.
  • 基因功能參考文獻(xiàn)：
    1. The ILEI appears to play a crucial role in mediating TGF-beta1-induced EMT through the Akt and ERK pathways, which may provide a therapeutic target for the treatment of fibrotic kidney diseases. PMID: 29336056
    2. Covalent dimerization of ILEI is associated with breast cancer metastasis. PMID: 28837266
    3. Data suggest that ILEI does not form interleukin-like four helix-bundle structures; ILEI exists as monomers and as covalent dimers; ILEI dimer exhibits a trans-linked domain swap converting an intramolecular disulfide to an intermolecular disulfide; dimeric ILEI appears to be involved in neoplastic invasiveness. PMID: 28751379
    4. Results demonstrate that phenotype switching regulates ILEI expression, and that ILEI regulates partial phenotype switching in MITF-low melanoma cell lines. PMID: 28545079
    5. Ectopic overexpression of UBE4A, but not UBE3C, in cells was downregulated in vitro migration and invasion in these cells. Cumulatively, our data reveals a novel post-translational regulatory mechanism of regulating ILEI1 expression, a protein required for metastatic progression in prostate cancer cells PMID: 27862841
    6. Study used immunohistochemical staining and biochemical fractionation to examine the regional distribution and subcellular localization of ILEI in mouse brains and in autopsy brains of patients with Alzheimer's disease; results suggest that a decline of ILEI expression may cause accumulation of Abeta in the brain and the eventual development of Alzheimer's disease. PMID: 27256505
    7. FAM3C expression was dramatically increased in esophageal squamous cell carcinoma and might serve as a valuable prognostic indicator for esophageal squamous cell carcinoma patients after surgery PMID: 26498278
    8. Altered subcellular ILEI localization strongly correlates with high tumor cell-associated uPAR protein expression. PMID: 25212966
    9. Our findings indicate that cytoplasmic ILEI expression is a potential marker of EMT and tumour progression in colorectal cancer. ILEI is an independent predictive factor associated with poor prognosis in colorectal cancer. PMID: 24738665
    10. ILEI could induce, as well as maintain, CD24(low)CD44(high) subpopulation in A549 cells treated with TGF-beta, which might explain its capability to induce metastatic progression. PMID: 24072492
    11. At the FAM3C gene locus where rs7776725 was located, we identified several other SNPs (rs4727922, rs1803389, rs718766 and rs7793554) that were also associated with BMD. PMID: 22159821
    12. A cytokine-like protein found in almost all tissues. PMID: 12160727
    13. ILEI is overexpressed and/or altered in intracellular localization in multiple human tumors. PMID: 16959614
    14. Data show that FAM3C is decreased in the CSF of temporal lobe epilepsy patients. PMID: 19109932

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  • 亞細(xì)胞定位：
    Secreted. Cytoplasmic vesicle. Note=Cytoplasmic in some cancer cells.
  • 蛋白家族：
    FAM3 family
  • 組織特異性：
    Present in most secretory epithelia (at protein level).
  • 數(shù)據(jù)庫鏈接：

    HGNC: 18664

    OMIM: 608618

    KEGG: hsa:10447

    STRING: 9606.ENSP00000353025

    UniGene: Hs.272375