Human Protein Induced Vitamin K Absence or Antagonist-II(PIVKA-II) ELISA Kit

產品詳情

文獻引用 (8) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人凝血酶原前體蛋白(PIVKA-II)酶聯(lián)免疫試劑盒（CSB-E13343h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、腹水、組織勻漿樣本中的PIVKA-II含量。PIVKA-II即異常凝血酶原，是一種腫瘤標志物。在維生素K缺乏或拮抗時產生，在肝癌等腫瘤細胞中高表達。研究機制上，它可反映肝細胞癌的生長和凝血功能異常，為肝癌早期診斷、病情監(jiān)測和預后評估提供重要參考。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于肝癌發(fā)生機制研究、抗腫瘤藥物研發(fā)、肝臟功能評估等科研領域，其多樣本兼容性特別適合臨床前研究中不同生物樣本的橫向對比分析，如腫瘤組織勻漿中異常凝血酶原表達檢測，或腹水樣本中的腫瘤標志物水平動態(tài)監(jiān)測，為維生素K代謝研究及肝病相關分子機制探索提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

PIVKA-II
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, ascitic fluid, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PIVKA-II in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-101
1:2	Average %	97
	Range %	86-101
1:4	Average %	96
	Range %	90-108
1:8	Average %	94
	Range %	82-103

回收率：

The recovery of human PIVKA-II spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-104
EDTA plasma (n=4)	97	91-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.