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  4. Human Protein S100-A7A(S100A7A) ELISA kit

Human Protein S100-A7A(S100A7A) ELISA kit

  • 中文名稱：
    人S100-A7A蛋白(S100A7A)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL020636HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人S100-A7A蛋白(S100A7A)酶聯(lián)免疫試劑盒（CSB-EL020636HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、尿液樣本中的S100A7A含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，可廣泛用于基礎(chǔ)醫(yī)學(xué)研究中S100A7A蛋白的功能探索，例如皮膚疾病模型中的炎癥標(biāo)志物檢測、腫瘤組織微環(huán)境相關(guān)蛋白表達(dá)分析，或通過體液樣本評估生物分子動(dòng)態(tài)變化，為分子機(jī)制研究提供靈敏可靠的工具。實(shí)驗(yàn)操作兼容常規(guī)酶標(biāo)儀檢測，支持多樣本類型靈活選擇，滿足科研場景中復(fù)雜樣本的檢測需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    S100A7A ELISA Kit; S100A15 ELISA Kit; S100A7L1 ELISA Kit; Protein S100-A7A ELISA Kit; S100 calcium-binding protein A15 ELISA Kit; S100 calcium-binding protein A7-like 1 ELISA Kit; S100 calcium-binding protein A7A ELISA Kit
  • 縮寫：
    S100A7A
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, urine
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human S100A7A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 93
    Range % 89-97
    1:2 Average % 92
    Range % 89-96
    1:4 Average % 98
    Range % 94-102
    1:8 Average % 91
    Range % 82-97
  • 回收率：
    The recovery of human S100A7A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 97 93-101
    EDTA plasma (n=4) 86 81-92
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    20 2.834 2.912 2.873 2.758
    10 2.175 2.017 2.096 1.981
    5 1.286 1.218 1.252 1.137
    2.5 0.654 0.664 0.659 0.544
    1.25 0.397 0.386 0.392 0.277
    0.625 0.249 0.242 0.246 0.131
    0.312 0.167 0.162 0.165 0.050
    0 0.115 0.114 0.115 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    May be involved in epidermal differentiation and inflammation and might therefore be important for the pathogenesis of psoriasis and other diseases.
  • 基因功能參考文獻(xiàn)：
    1. S100A15 retains the ability to coordinate zinc through incorporation of an exogenous ligand resulting in a unique zinc-binding site among S100 proteins. PMID: 22747601
    2. Molecular cloning and characterization of alternatively spliced S100A15 isoforms from psoriatic skin. PMID: 12923069
    3. The S100A15 protein has been expressed & purified to homogeneity in 2 crystal forms. Crystals of form I are triclinic. Crystals of form II are monoclinic & have a solvent content of 51.8%, corresponding to the presence of 1 monomer in the asymmetric unit. PMID: 16682778
    4. Overexpression of hS100A15 in chronic inflammatory skin diseases and regulation by inflammatory cytokines and calcium suggest that hS100A15 is involved in Th1-associated epithelial responses and epidermal maturation in normal and diseased human skin PMID: 17620096
    5. E. coli modulates S100A15 and IL-8 expression of keratinocytes by recognition through TLR4. PMID: 17625598
    6. study defines RAGE (receptor for advanced glycation end products) as the hS100A7 receptor, whereas hS100A15 functions through a Gi protein-coupled receptor PMID: 18606705

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    S-100 family
  • 組織特異性：
    Overexpressed in psoriasis.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 21657

    OMIM: 617427

    KEGG: hsa:338324

    UniGene: Hs.442337