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  4. Human Protein Wnt-2b(WNT2B) ELISA kit

Human Protein Wnt-2b(WNT2B) ELISA kit

  • 中文名稱：
    人Wnt-2b蛋白(WNT2B)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL026134HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人Wnt-2b蛋白(WNT2B)酶聯(lián)免疫試劑盒（CSB-EL026134HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的WNT2B含量。WNT2B是一個值得關注的靶點。它屬于WNT蛋白家族成員，在胚胎發(fā)育、細胞增殖與分化等過程中發(fā)揮重要作用。研究聚焦于其信號通路機制，探索其在腫瘤發(fā)生發(fā)展、組織再生修復等方面的作用，有望為相關疾病治療提供新思路。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，本試劑盒可靈敏檢測天然或重組蛋白濃度，配套預包被特異性捕獲抗體、生物素標記檢測抗體及鏈霉親和素 - HRP系統(tǒng)，提供標準化操作流程和預混試劑，支持科研人員開展發(fā)育生物學研究、腫瘤微環(huán)境分析、干細胞定向分化模型構建等基礎實驗。該產(chǎn)品兼容常規(guī)酶標儀檢測，適用于體外培養(yǎng)細胞、動物模型組織或生物液體樣本的靶蛋白表達水平分析，為探索Wnt信號網(wǎng)絡調(diào)控機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    WNT2B; WNT13; Protein Wnt-2b; Protein Wnt-13
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Stem Cells
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human WNT2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 87    
    Range % 83-94    
    1:2 Average % 98    
    Range % 94-101    
    1:4 Average % 102    
    Range % 98-105    
    1:8 Average % 95    
    Range % 90-101    
  • 回收率：
    The recovery of human WNT2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 91 85-105    
    EDTA plasma (n=4) 104 88-109    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    4000 2.513 2.355 2.434 2.285    
    2000 2.012 1.980 1.996 1.847    
    1000 1.220 1.222 1.221 1.072    
    500 0.725 0.721 0.723 0.574    
    250 0.417 0.421 0.419 0.270    
    125 0.298 0.282 0.290 0.141    
    62.5 0.227 0.224 0.226 0.077    
    0 0.148 0.150 0.149 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Plays a redundant role in embryonic lung development.
  • 基因功能參考文獻：
    1. Wnt2B co-operates with Frizzled7 to mediate mesenchymal to epithelial transition in colorectal cancer. PMID: 28560804
    2. Altered WNT2B expression in bladder wall fibroblasts alters expression of the apoptosis inductor TNFSF10. PMID: 29131138
    3. we identified two independent SNPs (i.e., WNT2B rs1175649 G>T and BTRC rs61873997 G>A) that showed a predictive role in CM-specific survival, with an effect-allele-attributed hazards ratio (adjusted hazards ratio) of 1.99 (95% confidence interval = 1.41-2.81, P = 8.10 x 10(-5)) and 0.61 (0.46-0.80, 3.12x10(-4)), respectively. PMID: 28499756
    4. A549-Luc-positive cells showed cytotoxicity following exposure to the Ad-shWnt2B vector. The percentage of viable cells was significantly lower in A549-Luc cells treated with Ad-shWnt2B than with Ad-scramble (p<0.01 versus control or Ad-scramble, respectively). PMID: 27793913
    5. ACSL5 mediates antiproliferative activities via Wnt2B palmitoylation with diminished Wnt activity. The molecular pathway is probably relevant for intestinal homeostasis, overwhelmed by other pathways in carcinogenesis. PMID: 25356045
    6. these results indicate that the high-expression levels of Gli1 and Wnt2B might play a pivotal role during tumorigenesis of pancreatic cancer PMID: 25120849
    7. Results showed that miR-185-3p contributs to radioresistance of nasopharyngeal carcinoma via modulation of WNT2B expression. PMID: 25297925
    8. secretion of WNT2B and WNT9B and stabilization of beta-catenin (CTNNB1) upon virus infection negatively regulate expression of representative inducible genes IFNB1, IFIT1 and TNF in a CTNNB1-dependent effector mechanism PMID: 23785285
    9. High Wnt2B overexpression is associated with ovarian cancer metastasis and drug resistance. PMID: 22635028
    10. This is the first report on differential regulation of WNT2 and WNT2B mRNAs in human cancer cell lines. PMID: 11712082
    11. Wnt-2b mRNA is expressed in human ovarian cancer cells PMID: 12072409
    12. REVIEW: Anti-WNT2B monoclonal antibodies, WNT2B RNAi compounds, or small molecule WNT2B inhibitors could be developed as novel therapeutic agents for gastric cancer and esophageal cancer in the field of clinical oncology. PMID: 16273293
    13. in addition to alternative promoters and RNA splicing, an alternative translation start in Wnt13B and Wnt13C mRNAs increases the complexity of both human wnt13 expression and functions PMID: 16407296
    14. Heplipin induced KG-1 cell apoptosis is related with Wntl3 and ATPase3. PMID: 17649721
    15. Hedgehog signals and bHLH transcription factors are involved in WNT2B upregulation, which is counteracted by BMP, WNT and Notch signals. PMID: 19360354

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  • 亞細胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted.
  • 蛋白家族：
    Wnt family
  • 組織特異性：
    Isoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidne
  • 數(shù)據(jù)庫鏈接：

    HGNC: 12781

    OMIM: 601968

    KEGG: hsa:7482

    STRING: 9606.ENSP00000358698

    UniGene: Hs.258575