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  4. Human Protein dopey-2(DOPEY2) ELISA kit

Human Protein dopey-2(DOPEY2) ELISA kit

  • 中文名稱：
    人dopey-2蛋白(DOPEY2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL007119HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人dopey-2蛋白(DOPEY2)酶聯(lián)免疫試劑盒（CSB-EL007119HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的DOPEY2含量。DOPEY2是一種蛋白質(zhì)編碼基因，與認(rèn)知和胚胎模式指定有關(guān)，位于早期內(nèi)體膜上。其可能參與調(diào)節(jié)貨物蛋白的膜運(yùn)輸，與ATP9A和MON2一起調(diào)節(jié)SNX3逆反器介導(dǎo)的內(nèi)體排序，避免WLS被溶酶體降解。DOPEY2的研究涉及其在細(xì)胞糖基化和囊泡運(yùn)輸中的影響，以及其在胚胎神經(jīng)發(fā)生中與Pcdh7協(xié)同調(diào)控神經(jīng)干細(xì)胞增殖和分化的作用。試劑盒檢測(cè)范圍為32 pg/mL-1200 pg/mL，支持科研工作者在神經(jīng)生物學(xué)、細(xì)胞代謝調(diào)控或疾病機(jī)制研究中開(kāi)展定量分析；適用于腦組織、外周血樣本或體外培養(yǎng)細(xì)胞模型中DOPEY2表達(dá)水平的高通量檢測(cè)，為探究其在神經(jīng)退行性疾病、代謝綜合征等病理過(guò)程中的作用提供數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    DOP1B ELISA Kit; C21orf5 ELISA Kit; DOPEY2 ELISA Kit; KIAA0933Protein dopey-2 ELISA Kit
  • 縮寫(xiě)：
    DOPEY2
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    32 pg/mL-1200 pg/mL
  • 靈敏度：
    8 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human DOPEY2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %86-97
    1:2Average %97
    Range %92-103
    1:4Average %85
    Range %81-89
    1:8Average %89
    Range %83-95
  • 回收率：
    The recovery of human DOPEY2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 104102-106
    EDTA plasma (n=4)9390-96
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    12002.313 2.451 2.382 2.335
    6401.754 1.802 1.778 1.731
    3201.012 1.032 1.022 0.975
    800.345 0.355 0.350 0.303
    320.173 0.174 0.174 0.127
    00.045 0.048 0.047
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    May play a role in regulating membrane trafficking of cargo proteins. Together with ATP9A and MON2, regulates SNX3 retromer-mediated endosomal sorting of WLS away from lysosomal degradation.
  • 基因功能參考文獻(xiàn)：
    1. The differentially expressed C21orf5 gene in the medial temporal-lobe system could play a role in Down syndrome. PMID: 12767918
    2. C21orf5 selective expression in the key brain structures for learning and memory suggests that C21orf5 overexpression could participate in mental retardation pathogenesis in Down syndrome patients. PMID: 16276086
    3. C21orf5, could play a role in brain morphogenesis and, when overexpressed, it could participate in neurological features and mental retardation observed in DS patients. PMID: 16303751
    4. Results quantify and statistically analyze, for the first time, DOPEY2 expression variations in different regions of the Down syndrome human fetal brains and to compare them to corresponding normal brains. PMID: 19460634
  • 亞細(xì)胞定位：
    Early endosome membrane. Golgi apparatus membrane; Peripheral membrane protein.
  • 蛋白家族：
    Dopey family
  • 組織特異性：
    Ubiquitously expressed. Overexpressed in lymphoblasts from Down syndrome patients.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 1291

    OMIM: 604803

    KEGG: hsa:9980

    STRING: 9606.ENSP00000382104

    UniGene: Hs.204575