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  4. Human Proteolysis Inducing Factor/Dermcidin(PIF/DCD)ELISA Kit

Human Proteolysis Inducing Factor/Dermcidin(PIF/DCD)ELISA Kit

  • 中文名稱：
    人蛋白水解誘導(dǎo)因子/皮離蛋白(PIF/DCD)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13626h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人蛋白水解誘導(dǎo)因子/皮離蛋白(PIF/DCD)酶聯(lián)免疫試劑盒（CSB-E13626h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、尿液、組織勻漿樣本中的DCD含量。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL，該產(chǎn)品適用于科研領(lǐng)域中對(duì)惡性腫瘤代謝異常、皮膚組織修復(fù)機(jī)制及相關(guān)炎癥性疾病的基礎(chǔ)研究，特別為探索PIF/DCD在生物體液或細(xì)胞分泌模型中的表達(dá)規(guī)律、評(píng)估實(shí)驗(yàn)性治療干預(yù)效果提供了可靠的檢測(cè)工具，廣泛應(yīng)用于分子病理學(xué)、藥理學(xué)及生物標(biāo)志物篩選等研究方向。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    AIDD ELISA Kit; DCD 1 ELISA Kit; dcd ELISA Kit; DCD-1 ELISA Kit; DCD_HUMAN ELISA Kit; Dermcidin ELISA Kit; DSEP ELISA Kit; HCAP ELISA Kit; PIF ELISA Kit; Preproteolysin ELISA Kit
  • 縮寫：
    DCD
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates, urine, tissue homogenates
  • 檢測(cè)范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PIF/DCD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %84
    Range %80-92
    1:200Average %97
    Range %91-105
    1:400Average %97
    Range %92-110
    1:800Average %93
    Range %86-98
  • 回收率：
    The recovery of human PIF/DCD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)9790-107
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.848 2.804 2.826 2.637
    202.335 2.369 2.352 2.163
    101.642 1.636 1.639 1.450
    50.906 0.919 0.913 0.724
    2.50.546 0.588 0.567 0.378
    1.250.343 0.356 0.350 0.161
    0.6250.252 0.263 0.258 0.069
    00.192 0.186 0.189 　
  • 本試劑盒所含材料：
    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human DCD antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled DCD antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問答

■ 常見問題解答
Q:

I am interested in buying a ELISA Kit for Human Dermcidin (DCD) but I need to detect this protein in Saliva Samples.,
Do you have any information about if the kit that you offer have been ever tested with saliva samples?

A:
Thanks for your inquiry.
We use this kit to detect human saliva sample with 1:10 dilution and get the value is 2.5--10ng/ml, then the final concentration is 25--100ng/ml.Pls let me know if you have any further questions. Thank you.

靶點(diǎn)詳情

  • 功能：
    DCD-1 displays antimicrobial activity thereby limiting skin infection by potential pathogens in the first few hours after bacterial colonization. Highly effective against E.coli, E.faecalis, S.aureus and C.albicans. Optimal pH and salt concentration resemble the conditions in sweat. Also exhibits proteolytic activity, cleaving on the C-terminal side of Arg and, to a lesser extent, Lys residues.; Survival-promoting peptide promotes survival of neurons and displays phosphatase activity. It may bind IgG.
  • 基因功能參考文獻(xiàn)：
    1. the present study suggests that dermcidin is a novel binding protein of lncRNA STCAT3, which serves an important role in the progress and clinical outcome of gastric cancer PMID: 30226544
    2. The results of this study suggest that these biomarker DCD can serve as a potential non-invasive early diagnosis platform reflecting PiB-PET imaging for Mild Cognitive Impairment and Alzheimer's Disease. PMID: 27392853
    3. this study shows that PIF regulates systemic immunity and targets protective regulatory and cytoskeleton proteins PMID: 26944449
    4. This is the first report of the presence of DCD in nasal mucosa and demonstration of DCD in clinical samples of human nasal secretions at clinically relevant concentrations, which may represent a novel arm of sinonasal airway innate defense. PMID: 27650261
    5. Identification of dermcidin in human sebaceous gland cells supports the concept that sebocytes play an important role in the innate immunity of the skin through the expression of antimicrobial peptides and specific lipids. PMID: 26718508
    6. Reduced DCD concentration in sweat in patients with inflammatory acne may permit proliferation of P. acnes in pilosebaceous units, resulting in progression of inflammatory acne. PMID: 25673161
    7. These findings imply that DCD promotes breast tumorigenesis via modulation of ERBB signaling pathways PMID: 25879571
    8. Dermcidin binds platelets and may inhibit the therapeutic action of aspirin following acute myocardial infarction. PMID: 25055737
    9. Data indicate that Y-P30/dermcidin expressed in placentas of first trimester pregnancies. PMID: 24969620
    10. Increased Dermcidin was also detected with immunoblotting. PMID: 24562771
    11. Crystal structure and functional mechanism of a human antimicrobial membrane channel PMID: 23426625
    12. major binding partners of LEKTI were found to be the antimicrobial peptide dermcidin and the serine protease cathepsin G and no kallikreins. PMID: 22588119
    13. Structure-activity analysis of the dermcidin-derived peptide DCD-1L, an anionic antimicrobial peptide present in human sweat. PMID: 22262861
    14. revealed that Nck1 SH2 domain binds to the phosphotyrosine residue at position 20 (Y20) of the DCD PMID: 21397687
    15. dermcidin was a novel platelet aggregating agent, and potentiated the ADP induced thrombosis in the animal model as well as acutely inhibited glucose induced insulin synthesis. PMID: 20809104
    16. There was a significant correlation between weight loss and survival in the PIF-positive non-small-cell lung cancer patients. PMID: 20837461
    17. Data show that dermcidin adopts a flexible amphipathic alpha-helical structure with a helix-hinge-helix motif, which is a common molecular fold among antimicrobial peptides. PMID: 20510021
    18. identified as antibiotic peptide secreted by sweat glands PMID: 11694882
    19. Our results confirmed previous findings indicating that dermcidin-1L could have promising therapeutic potentials and shed new light on the structure-function relationship of dermcidin-1L. PMID: 15670776
    20. Reduced expression of dermcidin in sweat of patients with atopic dermatitis may contribute to the high susceptibility of these patients to skin infections and altered skin colonization with Staphylococcus aureus. PMID: 15944307
    21. NF-kappaB and STAT3 are activated by proteolysis inducing factor in human Kupffer cells and monocytes PMID: 16142329
    22. Functional analysis indicated that proteolytic processing of DCD-1L by Cathepsin D in human sweat modulates the innate immune defense of human skin. PMID: 16354654
    23. Dermcidin may function as an oncogene in hepatic as well as breast cells; Glycosylation is not required, but the importance of asparagine residues suggests a role for the proteolysis-inducing factor core peptide domain. PMID: 16685272
    24. This work suggests that DCD might participate in the regulation of placental function by means of modulating the proteolytic cascades on the trophoblastic cell surface. PMID: 17448443
    25. Dermcidin is a proliferation and survival factor in prostate cancer cells subjected to stressors found in the prostate cancer microenvironment. PMID: 17626247
    26. These data suggest that the disorder and order transition may be relevant for some biological functions of PIF/DCD. PMID: 18058718
    27. There is a growing body of evidence illustrating dermcidin as an oncogene and its Y-P30 subunite as a survival factor (Review) PMID: 18403914
    28. Expression of dermcidin in human primary tumours appears highly variable and is not induced substantially by hypoxia/oxidative stress in cell line model systems PMID: 18594538
    29. pleiotrophin (PTN) and syndecan-2 and -3 as direct binding partners of Y-P30. PMID: 18599487
    30. IRAK-4, which is essential for virtually all TLR signalling, was suppressed, whereas the precursor for the antibiotic peptide Dermcidin was up-regulated in HIV-infected cells. PMID: 19703016

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  • 亞細(xì)胞定位：
    Secreted.
  • 組織特異性：
    Specifically and constitutively expressed in eccrine sweat gland cells. Secreted into the sweat at a concentration of 1-10 micrograms/ml.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 14669

    OMIM: 606634

    KEGG: hsa:117159

    UniGene: Hs.350570