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  4. Human Proto-oncogene Wnt-3(WNT3) ELISA kit

Human Proto-oncogene Wnt-3(WNT3) ELISA kit

  • 中文名稱：
    人原癌基因Wnt-3蛋白(WNT3)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL026135HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人原癌基因Wnt-3蛋白(WNT3)酶聯(lián)免疫試劑盒（CSB-EL026135HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的WNT3含量。WNT3 是一種重要的靶點。其屬于 Wnt 蛋白家族，在胚胎發(fā)育、組織穩(wěn)態(tài)維持等多個生理過程中發(fā)揮關(guān)鍵作用。研究機制上，它主要通過與受體結(jié)合激活 Wnt 信號通路，異常激活或抑制該通路與腫瘤、神經(jīng)系統(tǒng)疾病等多種病癥相關(guān)。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，的應(yīng)用場景涵蓋腫瘤生物學(xué)研究中的Wnt通路激活狀態(tài)評估、干細胞分化模型中信號分子動態(tài)監(jiān)測，以及疾病動物模型的組織特異性表達分析等領(lǐng)域。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Int 4 ELISA Kit; INT4 ELISA Kit; MGC131950 ELISA Kit; MGC138321 ELISA Kit; MGC138323 ELISA Kit; Proto-oncogene Int-4 homolog ELISA Kit; Proto-oncogene Wnt-3 ELISA Kit; wingless type MMTV integration site family member 3 ELISA Kit; Wnt 3 proto oncogene protein ELISA Kit; WNT 3 proto oncogene protein precursor ELISA Kit; wnt3 ELISA Kit; WNT3_HUMAN ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Stem Cells
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human WNT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %93
    Range %89-97
    1:2Average %105
    Range %100-110
    1:4Average %90
    Range %85-94
    1:8Average %84
    Range %80-88
  • 回收率：
    The recovery of human WNT3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8983-94
    EDTA plasma (n=4)9893-102
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.246 2.301 2.274 2.058
    51.705 1.642 1.674 1.458
    2.51.244 1.320 1.282 1.066
    1.250.905 0.873 0.889 0.673
    0.6250.541 0.542 0.542 0.326
    0.3120.435 0.467 0.451 0.235
    0.1560.312 0.325 0.319 0.103
    00.215 0.216 0.216
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis. Required for normal formation of the apical ectodermal ridge. Required for normal embryonic development, and especially for limb development.
  • 基因功能參考文獻：
    1. Demonstrate a significant change in miRNA profile dependent on the assisted reproductive technology outcome affecting Wnt pathway. PMID: 28971890
    2. High expression of WNT3 is associated with hepatocellular carcinoma. PMID: 28586038
    3. Our data demonstrated an important interdependence between TGF-beta and Wnt/beta-catenin pathways inducing EMT in HER2-overexpressing breast cancer cells. Twist served as a linkage between the two pathways during TGF-beta-induced EMT. A83-01 could inhibit the TGF-beta-initiated pathway interactions and enhance HER2-cells response to trastuzumab treatment PMID: 28337662
    4. YAP maintains human embryonic stem cells pluripotency by preventing WNT3 expression in response to Activin, thereby blocking a direct route to embryonic cardiac mesoderm formation. PMID: 29269485
    5. Wnt3 is associated with cholesterol-dependent domains PMID: 27463143
    6. analysis of WNT3 expression in CLL patients demonstrated that (i) untreated patients with more aggressive disease (with a notable exception of patients with 11q deletion) express less WNT3, (ii) WNT3 declines with disease progression in a significant proportion of patients and (iii) low WNT3 was identified as a strong independent marker indicating shorter treatment-free survival in CLL patients with IGHV mutation. PMID: 27651098
    7. PRKX, WNT3 and WNT16 genes, belonging to the WNT signaling pathway, are involved in the tumorigenic process of nodular basal cell carcinoma PMID: 27630294
    8. Two SNPs (rs3809857 and rs9890413) in the WNT3 gene were subjected to case-control and case-parent analysis PMID: 26505415
    9. WNT3 and membrane-associated beta-catenin regulate trophectoderm lineage differentiation in human blastocysts. PMID: 26108805
    10. WNT3 involvement in human bladder exstrophy and cloaca development in zebrafish PMID: 26105184
    11. Genome association study shows a highly conserved 32 kb intergenic region containing regulatory elements between WNT3 and WNT9B in patients with classic bladder exstrophy. PMID: 24852367
    12. mRNA level of WNT3 in hESCs correlates with their definitive ectoderm differentiation efficiency. PMID: 24052941
    13. Data show that the WNT/beta-CATENIN signaling cascade components, including WNT ligands WNT3 and WNT5A, is crucial for early odontogenesis. PMID: 24647585
    14. WNT3 inhibits cerebellar granule neuron progenitor proliferation and medulloblastoma formation via MAPK activation. PMID: 24303070
    15. Colorectal tumors express elevated levels of Wnt3 and GPR177. PMID: 24162018
    16. WNT3 and WNT5B are critical factors, secreted from mesenchymal cancer cells, for instigating the epithelial cancer cell invasion. PMID: 24344732
    17. Knockdown of Wnt3 by siRNA restored cytoplasmic expression of beta-catenin. PMID: 23071104
    18. The apoptotic index was significantly lower in high-Wnt3 tumors than in low-Wnt3 tumors (P=0.0245). PMID: 22070884
    19. study confirmed the involvement of polymorphisms in the WNT3 gene in NCL/P aetiology in the tested population. PMID: 22288914
    20. The findings of a significant association between lip and/or palate clefts and two markers in the WNT3 and COL11A2 genes were the most consistent and were observed in all groups analysed and stratified. PMID: 22112025
    21. SNP rs415430 in the WNT3 gene was not associated with the risk of development of Parkinson disease PMID: 21789800
    22. Extracellular domain of FZD7 (sFZD7) was tested for its functional activity to interact with Wnt3, its ability to inhibit Wnt3-mediated signaling. PMID: 21314951
    23. Abnormalities of the Wnt3/3a pathway (located in the apical ectodermal ridge) include tetra-amelia and loss of the distal phalanges/nails. PMID: 20709709
    24. Individuals carrying variant alleles in WNT3 presented an increased risk for cleft lip/palate (p = 0.0003; OR, 1.61; 95% CI, 1.29-2.02) in the Brazilian population studied. PMID: 20890934
    25. Results demonstrated significant up-regulation of WNT-3, WNT-4, WNT-5B, WNT-7B, WNT-9A, WNT-10A, and WNT-16B in patients with CLL compared to normal subjects. PMID: 19863181
    26. Regulation of WNT3 and WNT3A mRNAs in human cancer cell lines NT2, MCF-7, and MKN45 PMID: 11788904
    27. Wnt3 plays an important role in regulating characteristics and activity of stromal cells. PMID: 15588944
    28. The Wnt-3a triggers the interaction of LRP6 with caveolin and promotes recruitment of Axin to LRP6 phosphorylated by glycogen synthase kinase-3beta and that caveolin thereby inhibits the binding of beta-catenin to Axin. PMID: 16890161
    29. an internally truncated LRP5 receptor is strongly implicated in deregulated activation of the WNT/beta-catenin signaling pathway in hyperparathyroid tumors PMID: 18044981
    30. The results indicated that HBx induction in the CCL13-HBx stable cell line downregulated Wnt-3/beta-catenin expression and suppressed cell growth by repressing cell proliferation or triggering cell apoptosis. PMID: 18309246
    31. a functional interaction between Wnt3 and FZD7 leading to activation of the Wnt/beta-catenin signaling pathway in HCC cells and may play a role during hepatocarcinogenesis. PMID: 18313787
    32. CTLA-4 is a direct target of Wnt/beta-catenin signaling and is expressed in human melanoma tumors PMID: 18563180
    33. WNT3 regulates distinct internalization pathways of LRP6 to tune the activation of beta catenin signaling. PMID: 18606139
    34. mantle cell lymphoma highly and consistently expressed Wnt3 and Wnt10. PMID: 18787224
    35. WNT activity regulates the self-renewal of prostate cancer cells with stem cell characteristics independently of androgen receptor activity. PMID: 19365403

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  • 相關(guān)疾病：
    Tetraamelia syndrome, autosomal recessive (TETAMS)
  • 亞細胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted.
  • 蛋白家族：
    Wnt family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 12782

    OMIM: 165330

    KEGG: hsa:7473

    STRING: 9606.ENSP00000225512

    UniGene: Hs.445884