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  4. Human R-spondin-2(RSPO2) ELISA kit

Human R-spondin-2(RSPO2) ELISA kit

  • 中文名稱：
    人R脊椎蛋白2(RSPO2)酶聯免疫試劑盒
  • 貨號：
    CSB-EL020551HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人R脊椎蛋白2(RSPO2)酶聯免疫試劑盒（CSB-EL020551HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的RSPO2含量。RSPO2是一個重要靶點。其背景上，在多種生理過程中發(fā)揮作用。研究機制方面，它參與調控信號通路，如Wnt信號通路，對細胞的增殖、分化和組織發(fā)育等有影響，在腫瘤等疾病的發(fā)生發(fā)展機制研究中受到關注。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于體外研究RSPO2在腫瘤微環(huán)境中的調控機制、干細胞分化模型中的信號通路分析，以及利用多種生物樣本探索疾病發(fā)生發(fā)展相關的分子機制，為生命科學基礎研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    RSPO2 ELISA Kit; UNQ9384/PRO34209 ELISA Kit; R-spondin-2 ELISA Kit; Roof plate-specific spondin-2 ELISA Kit; hRspo2 ELISA Kit
  • 縮寫：
    RSPO2
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Developmental Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human RSPO2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %88
    Range %84-91
    1:2Average %99
    Range %94-102
    1:4Average %93
    Range %86-95
    1:8Average %94
    Range %89-98
  • 回收率：
    The recovery of human RSPO2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9286-95
    EDTA plasma (n=4)9589-99
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    101.974 1.913 1.944 1.792
    51.298 1.277 1.288 1.136
    2.50.739 0.754 0.747 0.595
    1.250.426 0.415 0.421 0.269
    0.6250.301 0.287 0.294 0.142
    0.3120.234 0.251 0.243 0.091
    0.1560.198 0.192 0.195 0.043
    00.154 0.150 0.152
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. During embryonic development, plays a crucial role in limb specification, amplifying the Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3, hence governing the number of limbs an embryo should form.
  • 基因功能參考文獻：
    1. results establish that RSPO2, without the LGR4/5/6 receptors, serves as a direct antagonistic ligand to RNF43 and ZNRF3, which together constitute a master switch that governs limb specification; these findings have direct implications for regenerative medicine and WNT-associated cancers PMID: 29769720
    2. RSPO2 is a novel target gene of the NOBOX key transcription factor, confirming its important role during the follicular growth in ovary. However, RSPO2 mutations are rare or uncommon in women with primary ovarian insufficiency. PMID: 28743298
    3. Novel targets ANTXR1 and RSPO2 were confirmed to be suppressed by miR-493 directly. PMID: 28651234
    4. RSPOs facilitate HSC activation and promote liver fibrogenesis by enhancing the Wnt pathway PMID: 27572318
    5. Role of RSPO2 in gastric cancer PMID: 28219935
    6. RSPO2 suppresses colorectal cancer metastasis by counteracting the Wnt5a/Fzd7-driven noncanonical Wnt pathway. PMID: 28600110
    7. genetic and functional data indicate that RSPO2 is a susceptibility gene for OPLL (Ossification of the Posterior Longitudinal Ligament of the Spine) PMID: 27374772
    8. Rspo2 was found to have a negative regulatory effect during oxidized low density lipoproteininduced macrophage apoptosis by regulating lipid uptake. PMID: 27571704
    9. data reveal a RSPO2-induced, LGR5-dependent Wnt signaling-negative feedback loop that exerts a net growth-suppressive effect on CRC cells PMID: 24476626
    10. results suggest that the expression of RSPO fusion transcripts is related to a subset of colorectal cancers arising in the Japanese population PMID: 24847761
    11. Wnt(high) cells were more likely to give rise to Wnt(high) progeny, tended to be more metastatic, and revealed higher levels of RSPO2 expression. PMID: 25769727
    12. These findings suggest that R-Spondin2 may promote hepatic stellate cell activation by enhancing the canonical Wnt pathway. PMID: 24852883
    13. Oocyte-derived R-spondin2 is a paracrine factor essential for primary ovarian follicle development PMID: 23407710
    14. using RNA-seq data, identification of multiple fusion transcripts including recurrent gene fusions involving R-spondin family members RSPO2 and RSPO3 that together occur in 10% of colon tumours PMID: 22895193
    15. Reduced Rspo-2 levels in osteoarthritis osteoblasts are responsible, at least in part, for their reduced Wnt/beta-catenin signaling and abnormal mineralization. PMID: 22127703
    16. There is a role for R-spondin2 in keratinocyte proliferation and epidermal thickening in keloid scarring. PMID: 21160497

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    R-spondin family
  • 數據庫鏈接：

    HGNC: 28583

    OMIM: 610575

    KEGG: hsa:340419

    STRING: 9606.ENSP00000276659

    UniGene: Hs.444834