Human Repetin(RPTN) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人Repetin蛋白(RPTN)酶聯(lián)免疫試劑盒（CSB-EL020501HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的RPTN含量。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，該產(chǎn)品適用于科研領域中對皮膚生理病理機制、角質形成細胞功能研究，以及化妝品或藥物對表皮屏障影響的體外評估等場景，可為皮膚生物學、疾病模型構建及相關轉化研究提供可靠的工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

RPTN ELISA Kit; Repetin ELISA Kit
縮寫：

RPTN
Uniprot No.：

Q6XPR3
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human RPTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	88-97
1:2	Average %	86
	Range %	80-92
1:4	Average %	96
	Range %	87-102
1:8	Average %	93
	Range %	89-97

回收率：

The recovery of human RPTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-98
EDTA plasma (n=4)	90	87-94

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Involved in the cornified cell envelope formation. Multifunctional epidermal matrix protein. Reversibly binds calcium.
基因功能參考文獻：
1. The single-nucleotide polymorphisms of CRNN (rs941934) and RPTN (rs3001978, rs28441202) may contribute to Atopic Dermatitis development, but further studies on a larger group of Atopic Dermatitis patients are needed to verify this assumption. PMID: 28219068
2. RPTN plays a potential role in emotional and cognitive processing; its decrease in serum may indicate its involvement in the pathogenesis of schizophrenia and bipolar disorder PMID: 25613293
亞細胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

S100-fused protein family
組織特異性：

Expression is scattered in the normal epidermis but strong in the acrosyringium, the inner hair root sheath and in the filiform papilli of the tongue.
數(shù)據(jù)庫鏈接：

HGNC: 26809

OMIM: 613259

KEGG: hsa:126638

STRING: 9606.ENSP00000317895

UniGene: Hs.376144