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中文名稱:人S100鈣結(jié)合蛋白A12/鈣粒蛋白C(S100A12)酶聯(lián)免疫試劑盒
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貨號:CSB-E13095h
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人S100鈣結(jié)合蛋白A12/鈣粒蛋白C(S100A12)酶聯(lián)免疫試劑盒(CSB-E13095h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的S100A12含量。S100A12屬于S100蛋白家族,在多種炎癥性疾病中發(fā)揮重要作用。研究發(fā)現(xiàn)其通過與晚期糖基化終末產(chǎn)物受體(RAGE)等相互作用,激活NF-κB等信號通路,參與炎癥反應、免疫調(diào)節(jié)等過程,是炎癥相關(guān)疾病潛在治療靶點。試劑盒檢測范圍為0.156 ng/ml-10 ng/ml,靈敏度為0.039ng/ml.。為研究S100A12在炎癥信號通路中的作用機制、探索其作為疾病相關(guān)生物標志物的潛力提供可靠工具,廣泛應用于免疫學、細胞生物學及病理生理學等基礎(chǔ)研究領(lǐng)域。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:CAAF1 ELISA Kit; CAGC ELISA Kit; Calcitermin ELISA Kit; Calcium-binding protein in amniotic fluid 1 ELISA Kit; Calgranulin C ELISA Kit; Calgranulin-C ELISA Kit; Calgranulin-related protein ELISA Kit; CGRP ELISA Kit; EN RAGE ELISA Kit; EN-RAGE ELISA Kit; ENRAGE ELISA Kit; Extracellular newly identified RAGE-binding protein ELISA Kit; migration inhibitory factor-related protein 6 ELISA Kit; MRP6 ELISA Kit; Neutrophil S100 protein ELISA Kit; p6 ELISA Kit; Protein S100 A12 ELISA Kit; S100 calcium binding protein A12 ELISA Kit; S100 calcium-binding protein A12 (calgranulin C) ELISA Kit; S100 calcium-binding protein A12 ELISA Kit; S100A12 ELISA Kit; S10AC_HUMAN ELISA Kit
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縮寫:S100A12
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:0.156 ng/ml-10 ng/ml
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靈敏度:0.039ng/ml.
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Signal Transduction
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human S100A12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:20
Average %
104
Range %
99-109
1:40
Average %
112
Range %
108-118
1:80
Average %
98
Range %
91-104
1:160
Average %
101
Range %
94-108
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回收率:
The recovery of human S100A12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
102
95-109
EDTA plasma (n=4)
99
92-106
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標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
10
2.846
2.694
2.770
2.609
5
2.337
2.367
2.352
2.191
2.5
1.679
1.748
1.714
1.553
1.25
1.247
1.229
1.238
1.077
0.625
0.737
0.721
0.729
0.568
0.312
0.512
0.533
0.523
0.362
0.156
0.303
0.284
0.294
0.133
0
0.164
0.158
0.161
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻
- Single-Cell RNA Sequencing of PBMCs Identified Junction Plakoglobin (JUP) as Stratification Biomarker for Endometriosis T Andrieu, A Duo, L Duempelmann, M Patzak,International Journal of Molecular Sciences,2024
- Identification of Neutrophil Extracellular Trap-Related Gene Expression Signatures in Ischemia Reperfusion Injury During Lung Transplantation: A Transcriptome Analysis and Clinical Validation W Wu,Journal of inflammation research,2024
- The potential pathogenic roles of S100A8/A9 and S100A12 in patients with MPO-ANCA-positive vasculitis X Bai,BMC immunology,2022
- Discovery, validation, and diagnostic ability of multiple protein-based biomarkers in saliva and gingival crevicular fluid to distinguish between health and periodontal diseases MM Grant,Journal of clinical periodontology,2022
- The pathogenic roles of S100A8/A9 and S100A12 in antineutrophil cytoplasmic antibody-associated vasculitis PC Xu,ResearchSquare,2022
- Clinical features and nasal inflammation in asthma and allergic rhinitis M Chen,Clinical and Experimental Immunology,2022
- High Serum S100A12 Levels Predict Poor Outcome After Acute Primary Intracerebral Hemorrhage SZ Qiu,Neuropsychiatric disease and treatment,2021
- Assessment of EN-RAGE, sRAGE and EN-RAGE/sRAGE as potential biomarkers in patients with autoimmune hepatitis R Wu,Journal of Translational Medicine,2020
- Serum S100A12 levels are correlated with clinical severity in patients with dermatomyositis-associated interstitial lung disease Lou Y,Journal of International Medical Research,2019
- Relation of postoperative serum S100A12 levels to delirium and cognitive dysfunction occurring after hip fracture surgery in elderly patients Li QH, et al,Brain and Behavior,2018
- Myeloid related proteins are up-regulated in autoimmune thyroid diseases and activate toll-like receptor 4 and pro-inflammatory cytokines in vitro Peng S.et al,Int Immunopharmacol,2018
- Serum S100A12 as a prognostic biomarker of severe traumatic brain injury Ma-JingFeng.et al,Clinica Chimica Acta,2018
- Diagnostic value of amniotic fluid inflammatory biomarkers for subclinical chorioamnionitis. Liu Y.et al,Int J Gynaecol Obstet.,2016
- Serum S100A12 and progression of coronary artery calcification over 4 years in hemodialysis patients Wang Y. et al,American Journal of Nephrology,2015
- Increased Plasma S100A12 Levels Are Associated With Diabetic Retinopathy and Prognostic Biomarkers of Macrovascular Events in Type 2 Diabetic Patients Dong N. et al,Invest Ophthalmol Vis Sci.,2015
- Polyunsaturated fatty acids augment tumoricidal action of 5-fluorouracil on gastric cancer cells by their action on vascular endothelial growth factor, tumor necrosis factor-a and lipid metabolism related factors Haining Yu. et al,ARCHIVES OF MEDICAL SCIENCE,2015
- Serum S100A12 concentrations are correlated with angiographic coronary lesion complexity in patients with coronary artery disease Liu J et al,Scand J Clin Lab Invest.,2013
- Serum S100A12 levels are associated with the presence and severity of obstructive sleep apnea syndrome in male patients Shi YK et al,Sleep Breath,2013
- Serum S100A12 Levels Are Correlated With the Presence and Severity of Coronary Artery Disease in Patients With Type 2 Diabetes Mellitus Zhao P et al,J Investig Med,2013
- S100A12 levels in synovial fluid may reflect clinical severity in patients with primary knee osteoarthritis Li-chun Wang et al,Biomarkers.,2013
相關(guān)產(chǎn)品
靶點詳情
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功能:S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its proinflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus.
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基因功能參考文獻:
- S100A12 activates NLPR3 inflammasomes to induce MUC5AC production in airway epithelial cells. ATP induces MUC5AC production in a mechanistically similar mode to S100A12. PMID: 29906464
- The results suggest that S100A12 does not participate in the induction of inflammation in dental pulp. However, RAGE can participate in the inflammation in the pulp of males. PMID: 28834384
- S100A12 was a significant predictor of lung alveolar infiltration (OR 2.60, 95%CI 1.35-5.00, p = 0.004). These results suggest that S100A12 has the potential to assess the extent of alveolar infiltration in Pulmonary tuberculosis. PMID: 27539060
- Results indicated that S100A12 could increase the expression of MMP-2, MMP-9, and vascular cell adhesion molecule 1 (VCAM-1) in HASMCs via activation of ERK1/2 signal pathway, which leads to injury of HASMCs. PMID: 28816402
- S100A12 binds to CD36 in the low nanomolar range at the CD36 thrombospondin-1 binding site. PMID: 27734162
- data on the antimicrobial activity of S100A12 have been reported. Proinflammatory role of S100A12 is supported by another newly found receptor, Toll-like receptor 4 (TLR4). PMID: 28110121
- Report role of fecal S100A12 assay in the diagnosis and management of inflammatory bowel disease. PMID: 28735301
- The aim of this mini-review was to outline the pleiotropic actions of S100A12 and to highlight the potential clinical importance of this protein in kidney and cardiovascular diseases. [review] PMID: 29080693
- serum levels had significant, positive correlations with intensive care unit length of stay, 28-day mortality, and in-hospital mortality after major abdominal surgery PMID: 27689623
- The binding interface between S100A12 and the V domain of RAGE has been identified and mapped. PMID: 27598566
- S100A12 functions as a proinflammatory cytokine and activates dermal fibroblasts, causing dermal fibrosis PMID: 27840235
- These data suggest that S100A12 is part of an innate and adaptive inducible antimicrobial network that contributes to host defense against mycobacteria in infected macrophages PMID: 27355424
- Among the investigated S100-proteins, S100A12 showed the closest association with disease activity and therapeutic response and might therefore provide a valuable biomarker for psoriasis PMID: 26333514
- the expression of S100A12 protein and mRNA was downregulated in a large number of clinical samples of GC. Low expression of S100A12 exhibited a marked propensity toward the clinicopathologic features such as tumor size, depth of invasion, TNM stage, Lauren classification, tumor cell differentiation, and poor survival in GC patients. PMID: 26638166
- Serum S100A12 was significantly higher in rheumatoid arthritis patients than controls, and was correlated with disease activity. PMID: 26767827
- an elevated serum level of S100A12 was an independent determinant of the progression of abdominal aortic calcification determined by lateral lumbar X-ray in Hemodialysis patients. PMID: 26914918
- Our findings indicate that chitotriosidase and S10012A are useful in diagnosis and detection of subclinical inflammation and/or assessment of disease activity in FMF patients. PMID: 26339162
- This is the first kinetics study of levels of RAGE main isoforms and ligands during ARDS. Elevated sRAGE, HMGB1 and S100A12, with decreased esRAGE and AGEs, were found to distinguish patients with ARDS from those without. PMID: 26274928
- serum level elevated in acute liver failure PMID: 25825217
- S100A12 is a biomarker of chronic heart failure that may also predict major cardiovascular events in patients with chronic heart failure. PMID: 25438075
- High mRNA expression of S100A12 is associated with bladder transitional cell carcinoma. PMID: 25854354
- These findings suggested that S100A12 is an effective marker for inflammatory diseases. PMID: 25650963
- High serum S100A12 expression is associated with poor response to therapy in Crohn's disease. PMID: 25625487
- Suggest that an elevated S100A12 level could play a crucial role in systemic inflammation and may be a promising biomarker for predicting perioperative complications in patients with thoracic aortic dissection. PMID: 24691129
- These assays showed that S100A12 is induced in response to Helicobacter pylori infection and inhibits bacterial growth and viability in vitro by binding nutrient zinc. PMID: 25964473
- S100A12 might participate in the damage of biliary epithelial cells and hepatocytes in primary biliary cirrhosis. PMID: 25313445
- Using a computational approach, the study investigated the modulation of protein structure by different ions in the solution, at different ionic strengths. PMID: 24944024
- Data indicate that resistin, S100A12 and soluble receptor for advanced glycation end products (sRAGE) are involved in the pathophysiology of Kawasaki disease (KD). PMID: 23171632
- The S100A12 protein was significantly associated with synovitis score in rheumatoid arthritis patients. PMID: 25282581
- highlight EN-RAGE as an inflammatory marker for future coronary heart disease (CHD) in a general population, beyond traditional CHD risk factors and inflammatory markers PMID: 25341801
- S100A12 is associated with duration of cardiopulmonary bypass, pulmonary inflammation, hypoxia and prolonged mechanical ventilation and may contribute to acute lung injury in cardiac surgery patients. PMID: 24887223
- The antimicrobial peptide calcitermin was isolated from human airway secretions and targets Gram-negative bacteria. PMID: 11522286
- Serum S100A12 concentrations are correlated with angiographic coronary lesion complexity in patients with coronary artery disease. PMID: 24341566
- Correlation of human S100A12 and high-sensitivity C-reactive protein as gingival crevicular fluid and serum markers of inflammation in chronic periodontitis and type 2 diabetes. PMID: 24378957
- Excessive expression of the S100A12 gene in uremic leukocytes is relevant to its increased serum concentration, particularly in those affected with cardiovascular disease. PMID: 23921255
- S100A12 and hBD2 correlate with the fecal microbiota thus linking the intestinal innate immune response to the bacterial colonization. PMID: 24307989
- EN-RAGE inflammatory ligand has an increased expression in Takayasu's arteritis patients. PMID: 23398829
- High S100A12 levels are associated with the presence and severity of coronary artery disease in patients with type 2 diabetes mellitus. PMID: 23609464
- High S100A12 expression is associated with intestinal inflammation and relapse in inflammatory bowel disease. PMID: 23377171
- The results indicate that plasma S100A12 level is an independent predictor for two-year all-cause mortality. A simple integer scoring system was therefore established for predicting mortality on the basis of plasma S100A12 levels. PMID: 23324110
- S100A12 could be a novel biomarker for predicting cardiovascular events for predicting MACE in patients with stable CAD. PMID: 22786469
- Fecal S100A12 levels were significantly higher in patients with severe necrotizing enterocolitis (NEC) at onset of disease and also, in contrast to fecal calprotectin, at 4-10 days before onset of NEC compared with unaffected reference infants. PMID: 22796048
- Significant relationship was found between MMP9 and HMGB1 and EN-RAGE in diabetic patients. PMID: 22549347
- Data indicate that S100A12 is up-regulated in Thoracic Aortic Aneurysm Dissection (TAAD) and may contribute to the pathogenesis of TAAD by initiating apoptosis of SMC, at least in part via increased oxidative stress. PMID: 22818064
- these data indicate the possible involvement of S100A12 in the development of osteoarthritis by up-regulating MMP-13 and VEGF via p38 MAPK and NF-kappaB pathways. PMID: 22609404
- Although S100A12 levels are not elevated in patients with decreased kidney function, a relation to markers of inflammatory disease is found. PMID: 21822023
- Levels of serum RAGE are reduced in patients with juvenile rheumatoid arthritis and correlate negatively with disease activity and S100A12 levels. PMID: 21724696
- Suggest that elevated plasma EN-RAGE and decreased sRAGE level could play a crucial role in systemic inflammation and carotid atherosclerosis in peritoneal dialysis patients. PMID: 21906738
- Transgenic expression of S100A12 in the lung of mice does not exacerbate lung inflammation in a model of OVA-induced allergic inflammation. PMID: 21418345
- plasma S100A12 protein level is strongly associated with the prevalence of cardiovascular disease in hemodialysis patients. PMID: 21258041
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亞細胞定位:Secreted. Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein. Note=Predominantly localized in the cytoplasm. Upon elevation of the intracellular calcium level, translocated from the cytoplasm to the cytoskeleton and the cell membrane. Upon neutrophil activation is secreted via a microtubule-mediated, alternative pathway.
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蛋白家族:S-100 family
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組織特異性:Predominantly expressed by neutrophils, monocytes and activated macrophages. Expressed by eosinophils and macrophages in asthmatic airways in regions where mast cells accumulate. Found in high concentrations in the serum of patients suffering from various
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