Human S100 calcium-binding protein A8/A9 complex(S100A8/A9)Elisa kit

產(chǎn)品詳情

文獻引用 (9) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人S100鈣結(jié)合蛋白A8/A9復(fù)合物（S100A8/A9）酶聯(lián)免疫試劑盒（CSB-E12149h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的S100A8/A9含量。S100A8/A9是鈣結(jié)合蛋白S100家族成員，參與炎癥反應(yīng)和細胞生長分化。研究發(fā)現(xiàn)，S100A8/A9在多種腫瘤中高表達，可能與腫瘤的發(fā)生發(fā)展密切相關(guān)，其具體機制尚需進一步研究。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，該產(chǎn)品適用于體外研究，如炎癥機制探索、疾病模型構(gòu)建、藥物干預(yù)效果評估或生物標志物相關(guān)性分析，為研究感染性病變、腫瘤進展、代謝綜合征等病理過程中 S100A8/A9 的動態(tài)變化提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

S100A8/A9
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human S100A8/A9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	80-92
1:2	Average %	95
	Range %	91-98
1:4	Average %	92
	Range %	87-98
1:8	Average %	95
	Range %	90-103

回收率：

The recovery of human S100A8/A9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	92	85-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.