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  4. Human SPARC-related modular calcium-binding protein 1(SMOC1) ELISA kit

Human SPARC-related modular calcium-binding protein 1(SMOC1) ELISA kit

  • 中文名稱：
    人SPARC相關(guān)模塊化鈣結(jié)合蛋白1(SMOC1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL021842HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人SPARC相關(guān)模塊化鈣結(jié)合蛋白1(SMOC1)酶聯(lián)免疫試劑盒（CSB-EL021842HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的SMOC1含量。SMOC1是一種與SPARC相關(guān)的模塊化鈣結(jié)合蛋白，其在小鼠的睪丸下降過(guò)程中發(fā)揮重要作用。SMOC1的表達(dá)受雄激素調(diào)節(jié)，并通過(guò)促進(jìn)睪丸支持組織的生長(zhǎng)和分化來(lái)調(diào)節(jié)睪丸的下降。研究顯示，SMOC1在調(diào)節(jié)睪丸支持組織的細(xì)胞增殖和分化中扮演關(guān)鍵角色，是雄激素作用的重要下游分子。試劑盒檢測(cè)范圍為78 pg/mL-5000 pg/mL，可為發(fā)育生物學(xué)研究、腫瘤微環(huán)境調(diào)控機(jī)制探索、纖維化疾病模型構(gòu)建等科研方向提供定量分析工具，特別適用于評(píng)估疾病模型中SMOC1的動(dòng)態(tài)表達(dá)水平或藥物干預(yù)實(shí)驗(yàn)的分子效應(yīng)檢測(cè)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Secreted modular calcium-binding protein 1 ELISA Kit; SMOC-1 ELISA Kit; SMOC1 ELISA Kit; SMOC1_HUMAN ELISA Kit; SPARC-related modular calcium-binding protein 1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    78 pg/mL-5000 pg/mL
  • 靈敏度：
    19.5 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SMOC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　 Sample Serum(n=4)
    1:1 Average % 96
    Range % 92-100
    1:2 Average % 103
    Range % 99-107
    1:4 Average % 90
    Range % 85-98
    1:8 Average % 92
    Range % 88-96
  • 回收率：
    The recovery of human SMOC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-101  
    EDTA plasma (n=4) 93 89-97  
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml OD1 OD2 Average Corrected
    5000 2.518 2.625 2.572 2.439
    2500 2.007 2.149 2.078 1.945
    1250 1.319 1.384 1.352 1.219
    625 0.675 0.629 0.652 0.519
    312 0.421 0.447 0.434 0.301
    156 0.263 0.271 0.267 0.134
    78 0.202 0.211 0.207 0.074
    0 0.130 0.135 0.133 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Plays essential roles in both eye and limb development. Probable regulator of osteoblast differentiation.
  • 基因功能參考文獻(xiàn)：
    1. Missense mutation in exon 3 of SMOC1 segregated with the Waardenburg anophthalmia syndrome in the Iranian family. PMID: 28807869
    2. This is the first report of Waardenburg anophthalmia syndrome (WAS) caused by a SMOC1 variant in a Pakistani population. The mutation identified in the present investigation extends the body of evidence implicating the gene SMOC-1 in causing WAS. PMID: 28085523
    3. SMOC binds to Pro-EGF, but does not induce Erk phosphorylation via the EGFR. PMID: 27101391
    4. IL-17A but not IL-22 suppresses the replication of hepatitis B virus by inducing the expression of MxA and OAS. PMID: 23274784
    5. SMOC1 provides a link between prenatal hormone exposure and digit ratio. PMID: 23263445
    6. Homozygosity mapping and subsequent targeted mutation analysis of a locus on 14q24.2 identified homozygous mutations in SMOC1 (SPARC-related modular calcium binding 1) in eight unrelated families with Waardenburg Anophthalmia syndrome. PMID: 21750680
    7. The present study thus identified SPARC related modular calcium binding 1 as a new cancer-associated protein capable of interacting with tenascin-C in vitro PMID: 21349332
    8. these findings indicate that SMOC1/Smoc1 is essential for ocular and limb development in both humans and mice. PMID: 21194678
    9. Waardenburg anophthalmia syndrome is genetically heterogeneous; a second was locus found on chromosome 14, and mutations in SMOC1 were shown also cause this syndrome. PMID: 21194680
    10. Analyzed the secretory protein profiles of BMSCs grown in osteogenic medium (OSM) and identified SPARC-related modular calcium-binding protein 1 (SMOC1), a member of the SPARC family, as a regulator of osteoblast differentiation of BMSCs. PMID: 20359165
    11. isolation of the novel gene SMOC-1 encoding a secreted modular protein containing an EF-hand calcium-binding domain; localization within basement membranes in kidney and skeletal muscle and expression in the zona pellucida surrounding the oocyte PMID: 12130637
    12. SMOC-1 is of physiological interest because it codes a secreted glycoprotein with five domains, each containing regions homologous to those on other proteins that mediate cell-matrix interactions. PMID: 17386346

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  • 相關(guān)疾?。?/div>
    Ophthalmoacromelic syndrome (OAS)
  • 亞細(xì)胞定位：
    Secreted, extracellular space, extracellular matrix, basement membrane. Note=In or around the basement membrane.
  • 組織特異性：
    Widely expressed in many tissues with a strongest signal in ovary. No expression in spleen.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 20318

    OMIM: 206920

    KEGG: hsa:64093

    STRING: 9606.ENSP00000355110

    UniGene: Hs.497349