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  4. Human Secretoglobin family 1D member 2(SCGB1D2) ELISA kit

Human Secretoglobin family 1D member 2(SCGB1D2) ELISA kit

  • 中文名稱：
    人分泌球蛋白家族1D成員2(SCGB1D2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL020814HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人分泌球蛋白家族1D成員2(SCGB1D2)酶聯(lián)免疫試劑盒（CSB-EL020814HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SCGB1D2含量。SCGB1D2是一種分泌珠蛋白，主要在汗腺細胞產(chǎn)生，具有潛在的抗萊姆病作用。研究發(fā)現(xiàn)，SCGB1D2能顯著抑制伯氏疏螺旋體生長，但其變異形式效果較差，可能與氨基酸序列變化有關(guān)。研究旨在利用SCGB1D2的抗菌特性，開發(fā)新型預防和治療萊姆病的方法。試劑盒檢測范圍為31.25 ng/mL-2000 ng/mL，適用于基礎(chǔ)科研中探索SCGB1D2在生殖生物學、炎癥性疾病或腫瘤微環(huán)境中的分子機制，也可用于評估動物模型或細胞實驗中該蛋白的表達動態(tài)變化，支持研究者高效開展SCGB1D2相關(guān)的蛋白質(zhì)組學或分子病理學研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    SCGB1D2 ELISA kit; LIPHB ELISA kit; LPNBSecretoglobin family 1D member 2 ELISA kit; Lipophilin-B ELISA kit
  • 縮寫：
    SCGB1D2
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    31.25 ng/mL-2000 ng/mL
  • 靈敏度：
    7.81 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SCGB1D2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:5Average %89
    Range %84-93
    1:10Average %98
    Range %84-102
    1:20Average %94
    Range %90-98
    1:40Average %103
    Range %99-110
  • 回收率：
    The recovery of human SCGB1D2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8680-90
    EDTA plasma (n=4)9288-97
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    20002.436 2.351 2.394 2.233
    10001.872 1.807 1.840 1.679
    5001.154 1.182 1.168 1.007
    2500.795 0.834 0.815 0.654
    1250.428 0.448 0.438 0.277
    62.50.362 0.341 0.352 0.191
    31.250.231 0.217 0.224 0.063
    00.163 0.159 0.161
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

靶點詳情

  • 功能：
    May bind androgens and other steroids, may also bind estramustine, a chemotherapeutic agent used for prostate cancer. May be under transcriptional regulation of steroid hormones.
  • 基因功能參考文獻：
    1. The data indicate that MGB1, MGB2 and LIPB mRNAs are expressed at low levels in human tissues but basal expression is upregulated in ovarian cancer. PMID: 24603286
    2. lipophilin B gene overexpression correlates with a less aggressive tumor phenotype and represents a novel ovarian carcinoma prognostic factor PMID: 23819652
    3. Serum antibodies to this protein are detected in late stage breast cancer patients. PMID: 12576445
    4. The present report demonstrates, for the first time, lipophilin B expression in the pituitary and its apparent down-regulation in pituitary adenomas. PMID: 15668787
    5. We conclude that expression of LPB is preferentially but not exclusively restricted to breast tissue. PMID: 17163411
    6. Ectopic overexpression of mammaglobin and lipophilin B did not affect the cell proliferation rate of Hs578T breast carcinoma cells in vitro. PMID: 18630503

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Secretoglobin family, Lipophilin subfamily
  • 組織特異性：
    Highest expression was found in skeletal muscle. Expressed as well in thymus, trachea, kidney, steroid responsive tissues (prostate, testis, uterus, breast and ovary) and salivary gland.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 18396

    OMIM: 615061

    KEGG: hsa:10647

    STRING: 9606.ENSP00000244926

    UniGene: Hs.204096