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  4. Human Serine/threonine-protein kinase LMTK3(LMTK3) ELISA kit

Human Serine/threonine-protein kinase LMTK3(LMTK3) ELISA kit

  • 中文名稱：
    人絲氨酸/蘇氨酸蛋白激酶LMTK3(LMTK3)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL013017HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人絲氨酸/蘇氨酸蛋白激酶LMTK3(LMTK3)酶聯(lián)免疫試劑盒（CSB-EL013017HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LMTK3含量。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，適用于基礎科研領域中對疾病機制探索、藥物靶點驗證或細胞信號通路研究，例如通過檢測不同處理條件下細胞或動物模型樣本中LMTK3的動態(tài)變化，分析其在腫瘤進展中的功能，或評估潛在治療藥物對該靶點的調控效應。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
  • 別名：
    LMTK3 ELISA Kit; KIAA1883 ELISA Kit; TYKLM3 ELISA Kit; Serine/threonine-protein kinase LMTK3 ELISA Kit; EC 2.7.11.1 ELISA Kit; Lemur tyrosine kinase 3 ELISA Kit
  • 縮寫：
    LMTK3
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    18.75 pg/mL-1200 pg/mL
  • 靈敏度：
    4.7 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human LMTK3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 90
    Range % 85-94
    1:2 Average % 97
    Range % 92-101
    1:4 Average % 89
    Range % 84-93
    1:8 Average % 94
    Range % 90-98
  • 回收率：
    The recovery of human LMTK3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 92 87-98
    EDTA plasma (n=4) 105 100-110
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1200 2.687 2.532 2.610 2.450
    600 1.994 2.018 2.006 1.846
    300 1.269 1.295 1.282 1.122
    150 0.716 0.728 0.722 0.562
    75 0.475 0.483 0.479 0.319
    37.5 0.321 0.307 0.314 0.154
    18.75 0.243 0.239 0.241 0.081
    0 0.158 0.162 0.160 　
  • 數(shù)據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Protein kinase which phosphorylates ESR1 (in vitro) and protects it against proteasomal degradation. May also regulate ESR1 levels indirectly via a PKC-AKT-FOXO3 pathway where it decreases the activity of PKC and the phosphorylation of AKT, thereby increasing binding of transcriptional activator FOXO3 to the ESR1 promoter and increasing ESR1 transcription. Involved in endocytic trafficking of N-methyl-D-aspartate receptors (NMDAR) in neurons.
  • 亞細胞定位：
    Membrane; Single-pass membrane protein. Cell projection, axon. Cell projection, dendrite. Golgi apparatus membrane. Note=Punctate pattern in cell projections.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family
  • 數(shù)據庫鏈接：

    HGNC: 19295

    UniGene: Hs.207426