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  4. Human Serum amyloid A-4 protein(SAA4) ELISA kit

Human Serum amyloid A-4 protein(SAA4) ELISA kit

  • 中文名稱：
    人血清淀粉樣蛋白A4(SAA4)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL020659HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人血清淀粉樣蛋白A4(SAA4)酶聯(lián)免疫試劑盒（CSB-EL020659HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的SAA4含量。試劑盒檢測(cè)范圍為15.6 ng/ml-1000 ng/ml，靈敏度為3.9 ng/ml。該產(chǎn)品適用于基礎(chǔ)醫(yī)學(xué)研究中SAA4蛋白的表達(dá)動(dòng)態(tài)分析、疾病動(dòng)物模型驗(yàn)證及炎癥相關(guān)分子機(jī)制探索，可為生物標(biāo)志物篩選、病理機(jī)制解析及藥物干預(yù)效果評(píng)估提供可靠數(shù)據(jù)支持，嚴(yán)格限用于科研領(lǐng)域。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Amyloid A; serum; 4 ELISA Kit; C SAA ELISA Kit; C-SAA ELISA Kit; Constitutively expressed serum amyloid A protein ELISA Kit; CSAA ELISA Kit; SAA 4 ELISA Kit; Saa4 ELISA Kit; SAA4 protein ELISA Kit; SAA4_HUMAN ELISA Kit; Serum amyloid A 4 protein ELISA Kit; Serum amyloid A-4 protein ELISA Kit; Serum amyloid A4 constitutive ELISA Kit
  • 縮寫(xiě)：
    SAA4
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    15.6 ng/ml-1000 ng/ml.
  • 靈敏度：
    3.9 ng/ml.
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human SAA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:100

    Average %

    97

    Range %

    93-101

    1:200

    Average %

    100

    Range %

    95-104

    1:400

    Average %

    95

    Range %

    89-99

    1:800

    Average %

    99

    Range %

    94-104

  • 回收率：

    The recovery of human SAA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    94

    89-97

    EDTA plasma (n=4)

    92

    88-97

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    1000

    2.845

    2.765

    2.805

    2.702

    500

    2.147

    2.089

    2.118

    2.015

    250

    1.422

    1.437

    1.430

    1.327

    125

    0.730

    0.712

    0.721

    0.618

    62.5

    0.394

    0.376

    0.385

    0.282

    31.2

    0.237

    0.242

    0.240

    0.137

    15.6

    0.153

    0.147

    0.150

    0.047

    0

    0.101

    0.105

    0.103

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    SAA4（serum amyloid A4）是SAA蛋白家族的一員，參與機(jī)體的免疫調(diào)節(jié)和炎癥反應(yīng)。最新研究表明，SAA4在某些腫瘤中表達(dá)升高，與腫瘤的發(fā)生和進(jìn)展密切相關(guān)。此外，SAA4也參與了許多炎癥和自身免疫疾病的發(fā)生和發(fā)展，成為新型治療策略的熱點(diǎn)研究對(duì)象。
  • 功能：
    Major acute phase reactant.
  • 基因功能參考文獻(xiàn)：
    1. Data identified SAA4 as a protein that was positively correlated with RF and RA. SAA4 may represent a novel prescreening marker for the diagnosis of RA. PMID: 28505104
    2. expressed in invasive trophoblast cells PMID: 24951172
    3. Six apolipoproteins (APOA1, APOA2, APOB, APOC2, APOC3, and APOE) were able to differentiate bladder cancer from hernia. SAA4 was significantly increased in bladder cancer subgroups, whereas ProEGF was significantly decreased in bladder cancer subgroups. PMID: 23631828
    4. studies provide the first evidence for a novel type of AA amyloidosis in which the fibrils were formed from a mutated SAA4 protein PMID: 20536400
    5. The sequences of 1478 and 1836 bp of the SAA1 and SAA4 5'-flanking regions were determined. PMID: 12410800

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    SAA family
  • 組織特異性：
    Expressed by the liver; secreted in plasma.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 10516

    OMIM: 104752

    KEGG: hsa:6291

    STRING: 9606.ENSP00000278222

    UniGene: Hs.1955