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Human Sialidase ELISA Kit

  • 中文名稱:
    人唾液酸酶酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E13811h
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人唾液酸酶酶聯(lián)免疫試劑盒(CSB-E13811h)為雙抗夾心法ELISA試劑盒,定量檢測血清、尿液、腦脊液(CSF)樣本中的NEU1含量。NEU1是一種神經(jīng)氨酸酶,主要在溶酶體中發(fā)揮功能,負責從糖蛋白和糖脂中去除硅酸殘基。其失調與多種疾病相關,包括溶酶體儲存疾病、自身免疫疾病和癌癥。NEU1在細胞信號傳導、免疫反應和病原體識別中起重要作用,其研究有助于揭示疾病機制和開發(fā)新治療策略。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL,該產品可應用于基礎研究領域,如探究唾液酸酶在感染性疾病、神經(jīng)系統(tǒng)疾病中的生物學功能,或評估其在體液中的動態(tài)變化規(guī)律,為疾病機制研究及生物標志物篩選提供可靠工具。試劑盒兼容血清、尿液及CSF等復雜體液樣本,具備良好的穩(wěn)定性和重復性,適用于實驗室級科研分析需求。本品僅用于科研,不用于臨床診斷,產品具體參數(shù)及操作步驟詳見產品說明書。
  • 別名:
    Acetylneuraminyl hydrolase ELISA Kit; exo-alpha-sialidase ELISA Kit; G9 sialidase ELISA Kit; Lysosomal sialidase ELISA Kit; N acetyl alpha neuraminidase 1 ELISA Kit; N-acetyl-alpha-neuraminidase 1 ELISA Kit; NANH ELISA Kit; NEU ELISA Kit; NEU1 ELISA Kit; NEUR1_HUMAN ELISA Kit; Neuraminidase 1 ELISA Kit; Neuraminidase ELISA Kit; SIAL1 ELISA Kit; sialidase 1 (lysosomal sialidase) ELISA Kit; Sialidase 1 ELISA Kit; Sialidase; lysosomal ELISA Kit; Sialidase-1 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, urine, cerebrospinal fluid (CSF)
  • 檢測范圍:
    0.312 ng/mL-20 ng/mL
  • 靈敏度:
    0.078 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human sialidase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %85
    Range %80-90
    1:2Average %94
    Range %91-98
    1:4Average %98
    Range %92-105
    1:8Average %95
    Range %86-100
  • 回收率:
    The recovery of human sialidase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9080-98
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1OD2AverageCorrected
    202.621 2.522 2.572 2.460
    102.212 2.112 2.162 2.050
    51.701 1.654 1.678 1.566
    2.51.257 1.197 1.227 1.115
    1.250.763 0.741 0.752 0.640
    0.6250.461 0.434 0.448 0.336
    0.3120.287 0.273 0.280 0.168
    00.115 0.109 0.112  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moieties from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage.
  • 基因功能參考文獻:
    1. in monocytes/macrophages, NEU1 is interconnected in a positive feedback loop with lipopolysaccharides and IL-1beta as enhancers of inflammation, and may therefore promote atherosclerosis and plaque instability PMID: 29371126
    2. Patients with minimally invasive and lepidic adenocarcinomas were categorized as low-risk group with high EGFR mutation rate; Patients with micro-papillary and solid adenocarcinomas were categorized as high-risk with lower EGFR mutation rate PMID: 29496311
    3. Transmembrane topology and dimerization ability of NEU1 have been reported. PMID: 27917893
    4. this work identified NEU1 as a novel biomarker for both diagnosis and prognosis in hepatocellular carcinoma PMID: 27602751
    5. Our combined data indicate that as HPMECs achieve confluence and CD31 ectodomains become homophilically engaged, multiple SFKs are activated to increase tyrosine phosphorylation of p120ctn, which in turn, functions as a cross-bridging adaptor molecule that physically couples NEU1 to CD31, permitting NEU1-mediated desialylation of CD31. PMID: 28343945
    6. TLR4 protein PMID: 29375116
    7. neuraminidase-1 (Neu1) in complex with matrix metalloproteinase-9 and G protein-coupled receptor tethered to RTKs and TLRs is identified as a major target in multistage tumorigenesis [review] PMID: 27029067
    8. EGFR protein, human is predictive of adjuvant chemo response. Also, It is a prognostic factor. PMID: 28862415
    9. Data suggest that mutations c.90delC (p.Tyr30Ter) and c.572A>T (p.Asp191Val) of the NFU1 gene probably underlie the pathogenesis of multiple mitochondrial dysfunction syndrome (MMDS). PMID: 28186588
    10. Elevated NEU1 expression alters functional activities of distinct lung cell types in vitro and recapitulates lymphocytic infiltration and collagen accumulation in vivo, consistent with mechanisms implicated in lung fibrosis. PMID: 26993524
    11. initial treatment with an EGFR-TKI is a reasonable option for patients presenting with EGFR mutation-positive NSCLC and a CM. PMID: 28237165
    12. miR-125b functions as an oncogene in gastric cancer and represents a new potential therapeutic target for gastric cancer. PMID: 27220320
    13. NEU1 siRNA can effectively inhibit proliferation, apoptosis, and invasion of human ovarian cancer cells by targeting lysosome and oxidative phosphorylation signaling, which can serve as a new target ovarian cancer treatment PMID: 26463994
    14. How the point mutations of the neuraminidase sequences affected the susceptibility of H9N2 virus to oseltamivir is still to be determined and deserve further investigations PMID: 26455375
    15. Our study shows that the 340-cavity is not an occasional or atypical domain in NA subtypes, and it has potential to function as a new hotspot for influenza drug binding. PMID: 26768362
    16. Basal Neu-1 catalytic activity is strongly increased in aged cells. PMID: 26086247
    17. Neu1 desialylation is a mechanism of Fc-independent platelet clearance in immune thrombocytopenia. PMID: 26185093
    18. Muc1 clearly plays a significant role in enhancing the hypoxia-inducible transcription factors protective pathway during ischemic insult and recovery in kidney epithelia PMID: 25925251
    19. This study analyzed patient cells with GM1 gangliosidosis and sialidosis. A novel mutation p.R347Q is identified in NEU1 gene. PMID: 25600812
    20. The lymphocyte levels of NEU1 and ST6GAL1 mRNA expression are significantly increased in erythremia. PMID: 25566667
    21. Insulin-induced phosphorylation of the insulin receptor is dependent on Neu1 sialidase activity. PMID: 24583283
    22. Sialidosis should be suspected and the NEU1 gene analyzed in patients with isolated action myoclonus presenting in adulthood in the absence of other typical clinical and laboratory findings. PMID: 24808020
    23. catalytically active NEU1 inhibits in vitro angiogenesis through desialylation of its substrate, CD31 PMID: 24550400
    24. Three novel mutations in the NEU1 gene in two Indian patients of sialidosis, one with the type I form and the other with the infantile type II form are reported here. PMID: 23391804
    25. NEU1 gene missense mutation was found in a patient diagnosed with type I sialoidosis. PMID: 23291686
    26. Weak anchorage of NEU1 and NEU3 to the plasma membrane and their loss during erythrocyte life could be a tool to preserve the cellular sialic acid content to avoid early aging of erythrocyte and processes of cell aggregation in the capillaries. PMID: 22903576
    27. Data indicate that sialidases Neu1 and Neu3 are present on sperm, and their activity is required for capacitation and zona pellucida binding. PMID: 22989879
    28. Data show that NEU1 was immunolocalized to both the plasma membrane and the perinuclear region, and NEU3 was detected both in the cytosol and nucleus. PMID: 22403397
    29. Neuraminidase 1 as a modulator of cell receptors. (Review) PMID: 21928149
    30. a novel Neu1 and MMP9 cross-talk in alliance with TLR4 on the cell surface that is essential for ligand activation of TLRs and subsequent cellular signaling. PMID: 21873432
    31. colocalized with CD18 in PMNs; could be a physiologic source of the enzymatic activity that removes sialyl residues on beta2 integrin and ICAM-1, resulting in their enhanced interaction PMID: 21551251
    32. Data show that Neu-1 siRNA blocks the process of GM(3)/LacCer conversion. PMID: 21103358
    33. Summary of NEU1 mutations found in sialidosis patients. PMID: 14517945
    34. type II sialidosis associated mutations in NEU1 affect lysosomal sialidase activity. PMID: 14695530
    35. Data show that the differentiation of monocytes into macrophages is associated with regulation of the expression of at least three distinct cellular sialidases, Neu1, 3, and 4, with specific up-regulation of the enzyme activity of only Neu1. PMID: 15885103
    36. Lysosomal sialidase (neuraminidase-1) is targeted to the cell surface to facilitate elastic fiber assembly PMID: 16314420
    37. effects of GLB1, PPCA and NEU1 gene mutations on elastogenesis in skin fibroblasts PMID: 16538002
    38. upregulation of the Neu1 expression is important for the primary function of macrophages and there is a link between Neu1 and the cellular immune response PMID: 16835219
    39. Activation of lymphocytes resulted in a ninefold increase in Neu1-specific activity after growth of cells in culture for 5 days.Cell surface Neu1 was found tightly associated with a subunit of protective protein/cathepsin A (PPCA). PMID: 17028199
    40. Female cervical mucus contains an endogenous menstrual cycle-related sialidase that could be involved in modifying the rheologic properties of mucus to favor sperm progression at fertilization. PMID: 17562335
    41. NEU1 is a negative regulator of lysosomal exocytosis. PMID: 18606142
    42. related to malignancy and may be potential targets for cancer diagnosis and therapy PMID: 18651674
    43. Neuraminidase caused the desialylation of both PDGF and IGF-1 receptors and diminished the intracellular signals induced by the mitogenic ligands PDGF-BB and IGF-2. PMID: 18772331
    44. results suggest that NEU1 is important in regulation of integrin beta4-mediated signaling, leading to suppression of metastasis PMID: 19151752
    45. findings show homozygous mutations of NEU1 544A-->G (Ser182Gly substitution) play a major role of the genotype and phenotype of Sialidosis type 1 PMID: 19473359
    46. The genetic characterization of the two patients showed one known [c. 679G > A (p.G227R)] NEU1 missense mutation (detected in P2), and the new c.807 + 1G > A splicing defect PMID: 19568825
    47. Results describe the hydrodynamic properties of PPCA, NEU1, and a complex of the two proteins and identified multiple binding sites on both proteins. PMID: 19666471
    48. occurrence of Neu3 in the inner membrane and Neu1 in the outer membrane of the nuclear envelope PMID: 19686243

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  • 相關疾病:
    Sialidosis (SIALIDOSIS)
  • 亞細胞定位:
    Lysosome membrane; Peripheral membrane protein; Lumenal side. Lysosome lumen. Cell membrane. Cytoplasmic vesicle. Lysosome. Note=Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles.
  • 蛋白家族:
    Glycosyl hydrolase 33 family
  • 組織特異性:
    Highly expressed in pancreas, followed by skeletal muscle, kidney, placenta, heart, lung and liver. Weakly expressed in brain.
  • 數(shù)據(jù)庫鏈接:

    HGNC: 7758

    OMIM: 256550

    KEGG: hsa:4758

    STRING: 9606.ENSP00000364782

    UniGene: Hs.520037