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  4. Human Small Dense Low Density Lipoprotein(sLDL)ELISA Kit

Human Small Dense Low Density Lipoprotein(sLDL)ELISA Kit

  • 中文名稱：
    人小而密低密度脂蛋白（sLDL）酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13350h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人小而密低密度脂蛋白（sLDL）酶聯(lián)免疫試劑盒（CSB-E13350h）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、唾液、尿液、組織勻漿樣本中的sLDL含量。sLDL（小而密低密度脂蛋白）是一種特殊的脂蛋白，其顆粒較小、密度較高，易被氧化且更容易進(jìn)入血管壁，在動(dòng)脈粥樣硬化等心血管疾病的發(fā)生發(fā)展中起重要作用。目前研究多聚焦于其致動(dòng)脈粥樣硬化的機(jī)制以及降低 sLDL 水平的干預(yù)措施。試劑盒檢測(cè)范圍為0.312 nmol/mL-20 nmol/mL，支持科研人員高效開展心血管疾病發(fā)病機(jī)制研究、降脂藥物作用效果評(píng)估、代謝綜合征相關(guān)模型構(gòu)建等實(shí)驗(yàn)。適用于體外實(shí)驗(yàn)環(huán)境下的基礎(chǔ)研究、藥理學(xué)研究及生物標(biāo)志物探索等科研場(chǎng)景。為縱向追蹤研究或大規(guī)模樣本分析提供便利。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 縮寫：
    sLDL
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, saliva, urine, tissue homogenates
  • 檢測(cè)范圍：
    0.312 nmol/mL-20 nmol/mL
  • 靈敏度：
    0.078 nmol/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human sLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 95  
    Range % 92-103  
    1:200 Average % 92  
    Range % 86-96  
    1:400 Average % 104  
    Range % 97-107  
    1:800 Average % 84  
    Range % 80-88  
  • 回收率：
    The recovery of human sLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 87 83-93  
    EDTA plasma (n=4) 92 88-96  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    nmol/ml OD1 OD2 Average    
    20 0.086 0.085 0.086    
    10 0.177 0.181 0.179    
    5 0.384 0.375 0.380    
    2.5 0.718 0.704 0.711    
    1.25 1.253 1.269 1.261    
    0.625 1.728 1.714 1.721    
    0.312 2.223 2.121 2.172    
    0 2.526 2.425 2.476    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

■ 常見問(wèn)題解答
Q:

What kind of dsLDL does the kit measure? (LDL-cholesterol? LDL-ApoB…. Etc?)
How does the kit differentiate de sdLDL from other LDL?

A:
Thanks for your inquiry.
This kit specifically use the sLDL raw material and detects the total sLDL(including the free SLDL or in combination with other proteins).
sLDL is the small dense Low-Density Lipoprotein,sd LDL or sLDL, which has much smaller grains and lower densities.
While those larger grains and lower densities LDL called large LDL.
You also can check more detailed informaiton on sLDL from this link: https://www.sciencedirect.com/science/article/pii/S0167527399001072 Pls let me know if you have any further questions. Thank you.