Human Soluble Elastin Fragments (sELAF) ELISA Kit

產(chǎn)品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人可溶性彈性蛋白片段(sELAF)酶聯(lián)免疫試劑盒（CSB-E14110h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的sELAF含量。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，適用于基礎(chǔ)科研中探究彈性蛋白代謝異常相關(guān)疾病模型、藥物干預(yù)效果評估或生物標(biāo)記物篩選，例如通過分析心血管疾病患者血清或肺纖維化動物模型組織勻漿中的sELAF水平，揭示其與疾病進展的關(guān)聯(lián)性。支持科研人員高效開展彈性蛋白代謝調(diào)控機制研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

sELAF
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 ng/mL-200 ng/mL
靈敏度：

0.78 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human sELAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	90-100
1:2	Average %	101
	Range %	93-105
1:4	Average %	95
	Range %	88-98
1:8	Average %	91
	Range %	87-94

回收率：

The recovery of human sELAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	91-102
EDTA plasma (n=4)	87	82-91

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.