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  4. Human Spondin-1(SPON1) ELISA kit

Human Spondin-1(SPON1) ELISA kit

  • 中文名稱：
    人脊椎蛋白-1(SPON1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL022599HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人脊椎蛋白-1(SPON1)酶聯(lián)免疫試劑盒（CSB-EL022599HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SPON1含量。SPON1（脊椎蛋白-1）是一種細胞外基質結構成分，參與細胞粘附和蛋白質結合活性的調節(jié)。其研究機制涉及在細胞外空間與膠原蛋白共定位，并可能參與淀粉樣蛋白前體蛋白的分解代謝過程。在疾病如阿爾茨海默病中，SPON1的表達和功能可能發(fā)生變化，影響疾病的發(fā)病機制。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，科研人員可通過探究SPON1在神經(jīng)退行性疾病模型中的表達特征，分析其在腫瘤微環(huán)境中的調控作用，或評估骨骼發(fā)育相關疾病中該蛋白的生物標志物價值；適用于體外實驗中的基礎機制研究、疾病模型分析及生物標志物篩選等科研場景，為探索SPON1的分子功能及其在病理生理過程中的作用提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    SPON1 ELISA kit; KIAA0762 ELISA kit; VSGPSpondin-1 ELISA kit; F-spondin ELISA kit; Vascular smooth muscle cell growth-promoting factor ELISA kit
  • 縮寫：
    SPON1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human spondin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %84
    Range %80-91
    1:2Average %86
    Range %81-92
    1:4Average %92
    Range %87-100
    1:8Average %95
    Range %88-101
  • 回收率：
    The recovery of human spondin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9790-104
    EDTA plasma (n=4)9386-97
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.504 2.463 2.484 2.365
    52.006 2.042 2.024 1.905
    2.51.506 1.479 1.493 1.374
    1.250.973 0.897 0.935 0.816
    0.6250.511 0.518 0.515 0.396
    0.3120.285 0.268 0.276 0.157
    0.1560.176 0.187 0.182 0.063
    00.121 0.117 0.119 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS. Major factor for vascular smooth muscle cell.
  • 基因功能參考文獻：
    1. gene expression experiment revealed that CYP2B6, SPON1, and GSG1L can be activated concomitantly through a constitutive androstane receptor (CAR) activation pathway PMID: 27010727
    2. SPON1 promotes metastatic progression through Fak and Src dependent pathway in human osteosarcoma. PMID: 26032498
    3. These data suggest that SPON1 may be associated with the differential rate of cognitive decline in Alzheimer disease. PMID: 23535033
    4. High SPON1 expression is associated with high-grade gliomas. PMID: 24158112
    5. Genome-wide scan of healthy human connectome discovers SPON1 gene variant influencing dementia severity PMID: 23471985
    6. downregulates osteoclast recruitment to the root side of periodontal tissue via low-density lipoprotein receptor-related protein 8 and inhibits differentiation of osteoclastic precursors PMID: 21488757
    7. These findings indicate that F-spondin regulates the differentiation of human cementoblast-like cells via BMP7 expression. PMID: 22244873
    8. The unique feature of F-spondin FS domain is the presence of three disulfide bonds associated with the N- and C-termini of the domain and a highly conserved N-linked glycosylation site. PMID: 21569239
    9. expression profile of VSGP/F-spondin identifies this molecule as a potential diagnostic marker or target for developing therapeutic strategies to treat ovarian carcinoma PMID: 16103746
    10. identified F-spondin as a promoting factor for cementoblastic differentiation PMID: 16965763
    11. The crystal structure of the reelin-N domain resolved to 2.0 A reveals a variant immunoglobulin-like fold and potential heparin-binding sites. PMID: 18602404
    12. These studies identify F-spondin as a novel protein in osteoarthritis cartilage, where it may act in situ at lesional areas to activate latent TGF-beta and induce cartilage degradation. PMID: 18780763

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  • 亞細胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 組織特異性：
    Highest expression in lung, lower expression in brain, heart, kidney, liver and testis, and lowest expression in pancreas, skeletal muscle and ovary. Not expressed in spleen.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 11252

    OMIM: 604989

    KEGG: hsa:10418

    UniGene: Hs.643864