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  4. Human Statherin(STATH) ELISA kit

Human Statherin(STATH) ELISA kit

  • 中文名稱：
    人富酪蛋白(STATH)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL022817HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人富酪蛋白(STATH)酶聯(lián)免疫試劑盒（CSB-EL022817HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、唾液樣本中的STATH含量。試劑盒檢測范圍為78 ng/mL-5000 ng/mL，適用于科研領(lǐng)域中對唾液成分分析、口腔疾病機制研究、牙釉質(zhì)生物礦化過程探索等方向，尤其可為齲齒風(fēng)險因素評估、口腔黏膜保護(hù)機制等基礎(chǔ)研究提供可靠數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    STAT_HUMAN ELISA Kit; STATH ELISA Kit; Statherin ELISA Kit; Statherin precursor ELISA Kit; STR ELISA Kit
  • 縮寫：
    STATH
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, saliva
  • 檢測范圍：
    78 ng/mL-5000 ng/mL
  • 靈敏度：
    19.5 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human STATH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %87-93
    1:2Average %95
    Range %91-104
    1:4Average %86
    Range %82-92
    1:8Average %96
    Range %92-101
  • 回收率：
    The recovery of human STATH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-97
    EDTA plasma (n=4)9994-104
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    50002.751 2.651 2.701 2.547
    25001.829 1.729 1.779 1.625
    12501.087 1.067 1.077 0.923
    6250.589 0.579 0.584 0.430
    312.50.382 0.372 0.377 0.223
    1560.265 0.255 0.260 0.106
    780.209 0.207 0.208 0.054
    00.154 0.153 0.154 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

靶點詳情

  • 功能：
    Salivary protein that stabilizes saliva supersaturated with calcium salts by inhibiting the precipitation of calcium phosphate salts. It also modulates hydroxyapatite crystal formation on the tooth surface.
  • 基因功能參考文獻(xiàn)：
    1. The total protein and statherin in the in-vivo AEP were different between eroded and non-eroded tooth surfaces of the same patient PMID: 28837608
    2. effects of statherin on intracellular calcium level and its subsequent related molecular alterations would give us new pathogenic aspect in oral carcinogenesis PMID: 25128293
    3. phenylalanine orientations in statherin bound to hydroxyapatite surfaces PMID: 22563672
    4. Data show that the full characterization of the statherin peptides generated facilitates the elucidation of their novel functional roles in the oral and gastro-intestinal environment. PMID: 20731414
    5. insight into the molecular interactions of statherin with hydroxyapatite surfaces PMID: 19678690
    6. inhibits calcium phosphate precipitation PMID: 12060866
    7. results suggest that a layer rich in statherin forms at the interface of saliva and air, and that the surface rheology developed is dependent upon protein interactions mediated by calcium PMID: 15769251
    8. In conclusion, statherin induces transition to yeast of Candida albicans hyphae and may thus contribute to the oral defense against candidiasis. PMID: 19799638

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Histatin/statherin family
  • 組織特異性：
    Secreted by parotid and submandibular glands.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 11369

    OMIM: 184470

    KEGG: hsa:6779

    STRING: 9606.ENSP00000246895

    UniGene: Hs.654495