Human Talin-1(TLN1) ELISA kit
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中文名稱:人踝蛋白(TLN1)酶聯(lián)免疫試劑盒
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貨號:CSB-EL023597HU
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人踝蛋白(TLN1)酶聯(lián)免疫試劑盒(CSB-EL023597HU)為 雙抗夾心法 ELISA試劑盒,定量 檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的TLN1含量。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL,適用于細(xì)胞生物學(xué)實(shí)驗(yàn)中的粘著斑動(dòng)態(tài)研究、腫瘤微環(huán)境分析、心血管疾病機(jī)制探索等科研場景,為體外研究TLN1在細(xì)胞力學(xué)響應(yīng)、病理生理過程中的功能提供可靠工具本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:ILWEQ ELISA Kit; Talin 1 ELISA Kit; Talin ELISA Kit; Talin-1 ELISA Kit; TLN 1 ELISA Kit; TLN ELISA Kit; Tln1 ELISA Kit; TLN1_HUMAN ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates, cell lysates
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檢測范圍:23.5 pg/mL-1500 pg/mL
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靈敏度:5.8 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Signal Transduction
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human TLN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 87 Range % 82-90 1:2 Average % 83 Range % 80-86 1:4 Average % 94 Range % 90-99 1:8 Average % 98 Range % 95-101 -
回收率:
The recovery of human TLN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 99 95-104 EDTA plasma (n=4) 102 98-106 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1500 2.428 2.468 2.448 2.284 750 1.651 1.698 1.675 1.511 375 1.104 1.100 1.102 0.938 187.5 0.662 0.657 0.660 0.496 94 0.477 0.498 0.488 0.324 47 0.355 0.362 0.359 0.195 23.5 0.261 0.271 0.266 0.102 0 0.165 0.163 0.164 -
貨期:3-5 working days
引用文獻(xiàn)
- Discovery, validation, and diagnostic ability of multiple protein-based biomarkers in saliva and gingival crevicular fluid to distinguish between health and periodontal diseases MM Grant,Journal of clinical periodontology,2022
- The activated β-integrin (CgβV) enhances RGD-binding and phagocytic capabilities of hemocytes in Crassostrea gigas Lv Z, et al,Fish & Shellfish Immunology,2019
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.
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基因功能參考文獻(xiàn):
- Low TLN1 expression is associated with invasion and metastasis in Hepatocellular Carcinoma. PMID: 29936723
- Calpain small subunit 1 (Capn4) overexpression increased the protein level of cleaved talin and and activated the focal adhesion kinase (FAK)/AKT/MAPK signaling in 786-O cells, while Capn4 silencing decreased the protein level of cleaved talin in Caki-1 cells. PMID: 29648579
- Talin-1 induces proliferation and migration of vascular smooth muscle cells obtained from patients with thoracic aortic dissection. PMID: 28637452
- Here, the authors show that cortical microtubule stabilization sites containing CLASPs, KIF21A, LL5beta and liprins are recruited to focal adhesions by the adaptor protein KANK1, which directly interacts with the major adhesion component, talin. Structural studies showed that the conserved KN domain in KANK1 binds to the talin rod domain R7. PMID: 27410476
- Vinculin head-tail interaction is required on soft substrates to destabilize vinculin and talin in FAs, and to allow hMSCs branching. Another module involves paxillin and FAK, which soft substrates also destabilize, but independently of vinculin head-tail interaction. This multi-modularity may be key to allow a versatile response to complex biomechanical cues. PMID: 27169142
- The central role of talin and vinculin in cell adhesions suggests that the disintegration of the tissue in atherosclerosis could be partially driven by downregulation of these genes, leading to loosening of cell-ECM interactions and remodeling of the tissue. PMID: 27816808
- Application of these techniques to new talin biosensors reveals an intramolecular tension gradient across talin-1 that is established upon integrin-mediated cell adhesion. The tension gradient is actomyosin- and vinculin-dependent and sensitive to the rigidity of the extracellular environment PMID: 28945706
- Our findings confirm the role of Talin-1 in carcinogenesis and provided a set of novel therapeutic targets for the treatment of hepatocellular carcinoma PMID: 28099903
- These data show that TLN1 can act as a viral restriction factor that suppresses hepatitis B virus replication, and suggest that the HBx relieves this restriction by inducing TLN1 degradation. PMID: 27775586
- The ERK pathway was responsible for these promoting effects of Talin1 knockdown in HCC cells. PMID: 28375585
- This study showed that serum sTalin-1 levels were associated with a sustained increase in disability after MS attack but not with serum anti-Talin-1 antibody levels. PMID: 28284333
- The Rap1-RIAM-talin axis of integrin activation and blood cell function PMID: 27207789
- These findings suggested that Talin-1 protein was significantly upregulated in PCa tissues compared with that of BPH tissue and Talin-1 expression was an independent predictor for lymph node metastasis and biochemical recurrence of PCa. PMID: 27442684
- Serum talin-1 had 97.7% sensitivity. PMID: 27644664
- Both TLN-1 and TLN-2 levels correlate with tumorigenicity in human HCC, indicating that these molecules constitute important molecular targets for the diagnosis and/or treatment of hepatocellular carcinoma . PMID: 26822056
- TLN1 significantly increases in refractory glioblastoma multiforme PMID: 26336988
- Applying correlative imaging to link live cell and fixed immunofluorescence data on a single cell basis, we related per cell talin-1 levels to per cell measures quantitatively defining an array of cellular properties. PMID: 26000342
- Disruption of the RIAM/lamellipodin-integrin-talin complex markedly impairs cell migration. PMID: 26419705
- Our data demonstrate that high expression of Talin-1 is associated with significantly poorer OS and poorer DMFS in NPC and depletion of Talin-1 expression inhibited NPC cell migration and invasion. Talin-1 may serve as novel prognostic biomarker in NPC. PMID: 25925041
- results identify talin as the primary determinant of FA nanoscale organization and suggest how multiple cellular forces may be integrated at adhesion sites PMID: 26283369
- The activation of vinculin by stretched talin induces a positive feedback that reinforces the actin-talin-vinculin association. PMID: 24452080
- Talin extension is a key step in sensing and responding to substrate stiffness at cell adhesion sites. PMID: 25142525
- Data (including data from molecular dynamic simulations) suggest specific interactions between glycoprotein GPIIb/GPIIIa complex transmembrane/C-terminal domains and talin-1 in cell membrane environment during platelet activation. PMID: 24677266
- phosphorylation of talin1 leading to beta1 integrin activation is a novel mechanism that increases metastatic potential of prostate cancer cells. PMID: 24793790
- Data suggest that anionic lipids (such as phosphatidylinositol phosphates) play crucial role in localization of peripheral membrane proteins (such as TLN1, auxilin-1, and PTEN [phosphatase and tensin homolog]). [review-like article] PMID: 25233425
- Low levels of Talin-1 expression are correlated with increased invasion and migration in liver cancer. PMID: 24761880
- Data indicate the role of vinculin in inducing the talin mediated integrin activation. PMID: 24446374
- MMP-2 regulates human platelet activation through hydrolysis of talin PMID: 24136115
- Talin has a role in regulating moesin-NHE-1 recruitment to invadopodia in breast cancer PMID: 24891603
- miR-9 plays a role as a tumor suppressor in OSC by suppressing TLN1 expression. PMID: 23722670
- These results indicate that mechanical forces loaded to focal adhesions (FAs) facilitate vinculin binding to talin at FAs. PMID: 24452377
- High Serum talin-1 expression is associated with hepatocellular carcinoma. PMID: 23886189
- Data indicate that KIF14 and TLN1 loss-of-function significantly enhanced chemosensitivity in four triple-negative breast cancer (TNBC) cell lines. PMID: 23479679
- Talin1 has unique expression versus talin 2 in the heart and modifies the hypertrophic response to pressure overload. PMID: 23266827
- CtsH-mediated processing of talin might promote cancer cell progression by affecting integrin alphaVbeta3 activation and adhesion strength PMID: 23204516
- Agonist stimulation, talin-1, and kindlin-3 are crucial for alpha(IIb)beta(3) activation in a human megakaryoblastic cell line, CMK. PMID: 23022222
- Kindlin-2 and talin head do not interact with one another but can bind simultaneously to the integrin beta(3) tail without enhancing or inhibiting the interaction of the other binding partner. PMID: 22648415
- Low Talin1 is associated with hepatocelluar carcinoma. PMID: 22471464
- Talin1 is required for inside-out activation of integrin beta1 during Bartonella henselae infection. PMID: 22045736
- FAK promotes talin recruitment to nascent adhesions occurring independently of talin binding to beta1 integrins. PMID: 22270917
- Talin1 is required for contact-dependent CD4-positive T cell proliferation in talin1 transgenic mice. Talin1 is not required for contact-independent CD4+ T cell proliferation. PMID: 22075696
- We investigated the role of talin-1, kindlin-3, and alpha-actinin-1 in the upregulation of alpha(4)beta(1) integrin affinity and consequent inflammatory leukocyte adhesive events PMID: 21911599
- Talin-1 upregulation is associated with disease progression in hepatocellular carcinoma. Thus, it may serve as a prognostic marker. PMID: 21846996
- Adhesions within the carcinoma matrix create a matrix environment in which exposure to cisplatin induces proliferation through the function of integrin beta, talin and FAK pathways that regulate NF-kB nuclear activity. PMID: 21720550
- Talin-1 and vinculin negatively affect tyrosine phosphorylation of paxillin, a novel positive regulator of HIV-1 infection, and impose an early block to infection by distinct retroviruses. PMID: 21763488
- We show that sequential cleavage of C-terminal amino acids from the beta(2) cytoplasmic tail of LFA-1, by CatX, enhances binding of the adaptor protein talin to LFA-1 and triggers formation of the latter's high-affinity form PMID: 21454358
- This review discusses the general function of talin 1 and talin 2, as well as vinculin/metavinulin, with emphasis on what is understood about their role in the cardiac myocyte and in whole heart. PMID: 19952892
- Studies suggest that the perturbed orientation of talin relative to the membrane in the F2 mutant would be expected to in turn perturb talin/integrin interactions. PMID: 20947017
- Data from talin crystal structure reveal a novel FERM domain with a linear domain arrangement, plus an additional domain F0 packed against F1. PMID: 20947018
- Rap1-mediated activation of alpha(M)beta(2) in macrophages shares both common and distinct features from Rap1 activation of alpha(IIb)beta(3) expressed in CHO cells. PMID: 20665668
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亞細(xì)胞定位:Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell surface. Cell junction, focal adhesion.
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