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  4. Human Tartrate Resistant Acid Phosphatase(TRAP)ELISA Kit

Human Tartrate Resistant Acid Phosphatase(TRAP)ELISA Kit

  • 中文名稱：
    人酒石酸鹽抵抗的酸性磷酸酶(TRAP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13415h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人酒石酸鹽抵抗的酸性磷酸酶(TRAP)酶聯(lián)免疫試劑盒（CSB-E13415h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TRAP含量。試劑盒檢測范圍為0.47 ng/mL-30 ng/mL，適用于體外骨代謝研究、破骨細胞活性評估、骨相關(guān)疾病機制探索以及藥物干預效果評價等科研領(lǐng)域，其高靈敏度的檢測性能可有效支持微量樣本中TRAP的動態(tài)變化分析，為骨生物學、細胞信號通路等基礎(chǔ)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
    TRAP
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.47 ng/mL-30 ng/mL
  • 靈敏度：
    0.12 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human TRAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 89-96  
    1:2 Average % 97  
    Range % 95-100  
    1:4 Average % 94  
    Range % 90-98  
    1:8 Average % 90  
    Range % 87-93  
  • 回收率：
    The recovery of human TRAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 92-100  
    EDTA plasma (n=4) 89 84-95  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    30 2.498 2.476 2.487 2.304  
    15 2.074 2.098 2.086 1.903  
    7.5 1.519 1.459 1.489 1.306  
    3.75 0.901 0.945 0.923 0.740  
    1.87 0.572 0.587 0.580 0.397  
    0.94 0.425 0.438 0.432 0.249  
    0.47 0.378 0.362 0.370 0.187  
    0 0.182 0.184 0.183 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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