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  4. Human Tetranectin(CLEC3B) ELISA kit

Human Tetranectin(CLEC3B) ELISA kit

  • 中文名稱：
    人四連接素(CLEC3B)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL005531HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人四連接素(CLEC3B)酶聯(lián)免疫試劑盒（CSB-EL005531HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CLEC3B含量。人四連接素（CLEC3B）是一種鈣離子依賴性凝集素，參與細(xì)胞黏附、分化調(diào)控及腫瘤抑制。其與纖溶酶原和kringle 4結(jié)合，可能涉及胞吐作用相關(guān)分子包裝和眼部生理學(xué)。研究顯示，CLEC3B在多種癌癥中表達(dá)下調(diào)，與腫瘤侵襲、轉(zhuǎn)移和免疫微環(huán)境相關(guān)，具有潛在的診斷和預(yù)后價(jià)值。試劑盒檢測(cè)范圍為1.56 ng/ml- 100 ng/ml，靈敏度為0.39 ng/ml。支持科研人員在體外實(shí)驗(yàn)中高效檢測(cè)目標(biāo)蛋白的表達(dá)變化，適用于基礎(chǔ)醫(yī)學(xué)研究中疾病機(jī)制探索、生物標(biāo)志物篩選以及藥物干預(yù)效果評(píng)估等場(chǎng)景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    C type lectin domain family 3 member B ELISA Kit; C-type lectin domain family 3 member B ELISA Kit; Clec3b ELISA Kit; Plasminogen kringle 4 binding protein ELISA Kit; Plasminogen kringle 4-binding protein ELISA Kit; TETN_HUMAN ELISA Kit; Tetranectin (plasminogen binding protein) ELISA Kit; Tetranectin ELISA Kit; TN ELISA Kit; TNA ELISA Kit
  • 縮寫：
    CLEC3B
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    1.56 ng/ml- 100 ng/ml
  • 靈敏度：
    0.39 ng/ml
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human CLEC3B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:100

    Average %

    107

    Range %

    103-112

    1:200

    Average %

    101

    Range %

    94-107

    1:400

    Average %

    95

    Range %

    88-101

    1:800

    Average %

    99

    Range %

    94-105

  • 回收率：

    The recovery of human CLEC3B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    89-100

    EDTA plasma (n=4)

    99

    92-106

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    100

    2.861

    2.734

    2.798

    2.655

    50

    1.897

    1.867

    1.882

    1.739

    25

    1.111

    1.058

    1.084

    0.942

    12.5

    0.649

    0.629

    0.639

    0.496

    6.25

    0.420

    0.399

    0.410

    0.267

    3.12

    0.290

    0.283

    0.287

    0.144

    1.56

    0.209

    0.204

    0.206

    0.064

    0

    0.141

    0.145

    0.143

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    CLEC3B是一種由CLEC3B基因編碼的蛋白質(zhì)，也被稱為牙齦溝液蛋白GCP-2。CLEC3B在人體免疫系統(tǒng)中發(fā)揮著重要作用，可通過識(shí)別病原體或潛在損傷信號(hào)，介導(dǎo)免疫反應(yīng)。最近的研究表明，CLEC3B還可能與其他生物學(xué)過程有關(guān)，如肝臟再生、炎癥反應(yīng)和腫瘤的生長(zhǎng)和轉(zhuǎn)移等。此外，CLEC3B的檢測(cè)也被用于診斷某些疾病和監(jiān)測(cè)其疾病進(jìn)程。
     
  • 功能：
    Tetranectin binds to plasminogen and to isolated kringle 4. May be involved in the packaging of molecules destined for exocytosis.
  • 基因功能參考文獻(xiàn)：
    1. Low CLEC3B expression is associated with clear cell renal cell carcinoma. PMID: 30066941
    2. The present study identified the CLEC3B p.S106G as a novel longevity-associated variant, raising the novel hypothesis that tetranectin, encoded by CLEC3B, plays a role in human longevity and aging PMID: 27154906
    3. TMEM88, CCL14, and CLEC3B genes were stable and available in predicting the survival and palindromia time of hepatocellular carcinoma. These genes could function as potential prognostic genes contributing to improve patients' outcomes and survival PMID: 28718365
    4. study suggests that increased serum TN level is associated with the presence and severity of diseased coronary arteries in patients with stable CAD. PMID: 26621497
    5. results predict that a positive TN expression of both tumour tissue and serum points to a more favourable outcome for ovarian cancer patients PMID: 25846370
    6. analyze the immunohistochemical localization of tetranectin in gastric adenocarcinomas and the adjacent tissues of the wall of the stomach PMID: 11962752
    7. Tetranectin may play an important role in the survival of islets in the liver after islet transplantation. PMID: 15848710

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  • 亞細(xì)胞定位：
    Secreted.
  • 組織特異性：
    Found in plasma.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 11891

    OMIM: 187520

    KEGG: hsa:7123

    STRING: 9606.ENSP00000296130

    UniGene: Hs.476092