Human Thromboxane B2,TX-B2 ELISA Kit

產品詳情

文獻引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人血栓素B2(TXB2)酶聯免疫試劑盒（CSB-E08046h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的TX-B2含量。TX-B2，即血栓素B2，是一種由血小板合成的脂質過氧化物，參與調節(jié)血管收縮和血小板聚集。其背景研究涉及血栓形成和心血管疾病。研究機制主要關注TX-B2在血栓形成過程中的作用，以及如何通過調節(jié)TX-B2水平來預防和治療相關疾病。試劑盒檢測范圍為9.38 pg/mL-600 pg/mL，該產品廣泛應用于心血管疾病機制研究、抗血小板藥物藥效評估、炎癥信號通路探索等科研領域，例如通過檢測阿司匹林干預后血小板釋放的 TXB2 水平變化，或分析動脈粥樣硬化模型動物組織中的血栓素代謝動態(tài)本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

TX-B2
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

9.38 pg/mL-600 pg/mL
靈敏度：

2.34 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TX-B2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
Range %	80-105
1:2	Average %	95
Range %	85-108
1:4	Average %	106
Range %	90-112
1:8	Average %	95
Range %	80-110

回收率：

The recovery of human TX-B2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	80-110
EDTA plasma (n=4)	98	90-108

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.