Human Transmembrane protein 119(TMEM119) ELISA kit

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產(chǎn)品詳情

產(chǎn)品描述：

人跨膜蛋白119(TMEM119)酶聯(lián)免疫試劑盒（CSB-EL023686HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的TMEM119含量。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于體外培養(yǎng)的小膠質(zhì)細(xì)胞模型中蛋白表達(dá)水平檢測、動(dòng)物腦組織炎癥狀態(tài)評估，或探索外周血中TMEM119與神經(jīng)系統(tǒng)疾病潛在關(guān)聯(lián)的科研場景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

TMEM119 ELISA Kit; PSEC0199 ELISA Kit; UNQ731/PRO1415 ELISA Kit; Transmembrane protein 119 ELISA Kit; Osteoblast induction factor ELISA Kit; OBIF ELISA Kit
縮寫：

TMEM119
Uniprot No.：

Q4V9L6
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TMEM119 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	107
	Range %	103-110
1:2	Average %	88
	Range %	84-92
1:4	Average %	107
	Range %	103-110
1:8	Average %	90
	Range %	87-93

回收率：

The recovery of human TMEM119 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	100-108
EDTA plasma (n=4)	88	84-93

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Plays an important role in bone formation and normal bone mineralization. Promotes the differentiation of myoblasts into osteoblasts. May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway. Upregulates the expression of ATF4, a transcription factor which plays a central role in osteoblast differentiation. Essential for normal spermatogenesis and late testicular differentiation.
基因功能參考文獻(xiàn)：
1. TMEM119 expression in osteosarcoma tissues is positively correlated with cell cycle, apoptosis, metastasis and TGF-beta signaling. PMID: 28496199
2. TMEM119 serves as a reliable microglial marker that discriminates resident microglia from blood-derived macrophages in the human brain PMID: 26250788
3. qPCR showed that TMEM119 expression was highly enriched in temporal lobe CD11b+ cells over unpurified whole brain, and barely detectable in peripheral blood leukocytes. PMID: 26884166
亞細(xì)胞定位：

Cell membrane; Single-pass type I membrane protein. Cytoplasm. Endoplasmic reticulum membrane.
組織特異性：

Expressed in brain microglia (at protein level). Elevated expression levels seen in the brain of patients with Alzheimer disease. Expressed by osteoblast-like cells in bone tissues and follicular dendritic cells in lymphoid tissues.
數(shù)據(jù)庫鏈接：

HGNC: 27884

KEGG: hsa:338773

UniGene: Hs.449718