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  4. Human Transmembrane protein 95(TMEM95) ELISA kit

Human Transmembrane protein 95(TMEM95) ELISA kit

  • 中文名稱：
    人跨膜蛋白95(TMEM95)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL023899HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人跨膜蛋白95(TMEM95)酶聯(lián)免疫試劑盒（CSB-EL023899HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TMEM95含量。人跨膜蛋白95（TMEM95）是一種精子膜蛋白，其敲除在小鼠中會(huì)導(dǎo)致男性特異性不育表型。研究發(fā)現(xiàn)，TMEM95與卵子膜結(jié)合，促進(jìn)精子與卵子的膜融合，其作用機(jī)制尚待進(jìn)一步研究。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，本試劑盒通過(guò)高特異性配對(duì)抗體實(shí)現(xiàn)精準(zhǔn)檢測(cè)，可兼容多種生物樣本類型，適用于基礎(chǔ)科研中探索TMEM95在生殖系統(tǒng)生理功能、疾病模型中的表達(dá)變化，或結(jié)合其他分子標(biāo)記物解析相關(guān)調(diào)控機(jī)制。其寬泛的檢測(cè)范圍可滿足不同濃度樣本的定量需求，為體外實(shí)驗(yàn)、動(dòng)物模型或組織樣本的蛋白表達(dá)研究提供可靠工具，助力生殖健康、發(fā)育生物學(xué)等領(lǐng)域的分子機(jī)制探索。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    TMEM95 ELISA Kit; UNQ9390/PRO34281 ELISA Kit; Transmembrane protein 95 ELISA Kit
  • 縮寫：
    TMEM95
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human TMEM95 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %90-101
    1:2Average %100
    Range %95-105
    1:4Average %97
    Range %92-104
    1:8Average %93
    Range %89-96
  • 回收率：
    The recovery of human TMEM95 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9387-98
    EDTA plasma (n=4)10095-105
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.235 2.378 2.307 2.115
    5001.648 1.652 1.650 1.458
    2501.091 1.123 1.107 0.915
    1250.712 0.738 0.725 0.533
    62.50.488 0.496 0.492 0.300
    31.20.375 0.401 0.388 0.196
    15.60.267 0.283 0.275 0.083
    00.191 0.193 0.192 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Sperm protein required for fusion of sperm with the egg membrane during fertilization.
  • 亞細(xì)胞定位：
    Cytoplasmic vesicle, secretory vesicle, acrosome membrane; Single-pass type I membrane protein.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 27898

    OMIM: 617814

    KEGG: hsa:339168

    STRING: 9606.ENSP00000331466

    UniGene: Hs.259432