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中文名稱:人髓系細胞觸發(fā)受體-1(TREM-1)酶聯(lián)免疫試劑盒
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貨號:CSB-E04836h
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規(guī)格:96T/48T
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價格:¥3200/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人髓系細胞觸發(fā)受體-1(TREM-1)酶聯(lián)免疫試劑盒(CSB-E04836h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的TREM1含量。TREM1是重要免疫調(diào)節(jié)靶點。它在炎癥反應(yīng)中扮演關(guān)鍵角色,激活后可放大炎癥信號。目前研究圍繞其信號通路機制展開,旨在探究如何調(diào)控其活性以干預(yù)炎癥相關(guān)疾病,有望為感染性、自身免疫性等疾病治療提供新策略。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL,適用于體外科學(xué)研究,可用于探索炎癥相關(guān)疾病的分子機制、評估實驗動物模型中的炎癥狀態(tài)或篩選調(diào)控TREM - 1表達的藥物化合物,為免疫學(xué)、病理學(xué)及藥理學(xué)領(lǐng)域的基礎(chǔ)研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:CD354 ELISA Kit; OTTMUSP00000018206 ELISA Kit; TREM 1 ELISA Kit; TREM-1 ELISA Kit; TREM1 ELISA Kit; TREM1_HUMAN ELISA Kit; Triggering receptor expressed on monocytes 1 ELISA Kit; Triggering receptor expressed on myeloid cells 1 ELISA Kit; Triggering receptor TREM 1 ELISA Kit; Triggering receptor TREM1 ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:31.25 pg/mL-2000 pg/mL
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靈敏度:7.81 pg/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Immunology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human TREM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 97 Range % 92-102 1:2 Average % 90 Range % 82-97 1:4 Average % 104 Range % 99-110 1:8 Average % 86 Range % 82-93 -
回收率:
The recovery of human TREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 84-96 EDTA plasma (n=4) 96 90-101 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 2000 2.529 2.752 2.641 2.538 1000 2.038 2.079 2.059 1.956 500 1.304 1.389 1.347 1.244 250 0.701 0.733 0.717 0.614 125 0.441 0.455 0.448 0.345 62.5 0.302 0.326 0.314 0.211 31.25 0.205 0.201 0.203 0.100 0 0.104 0.101 0.103 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻
- Evaluation of serum-soluble triggering receptor expressed on myeloid cells-1 as a novel marker in the diagnosis of ventilator-associated pneumonia in adults Wassila Morsy Mohamed. et al,Egyptian Journal of Bronchology,2015
相關(guān)產(chǎn)品
靶點詳情
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功能:Cell surface receptor that plays important roles in innate and adaptive immunity by amplifying inflammatory responses. Upon activation by various ligands such as PGLYRP1, HMGB1 or HSP70, multimerizes and forms a complex with transmembrane adapter TYROBP/DAP12. In turn, initiates a SYK-mediated cascade of tyrosine phosphorylation, activating multiple downstream mediators such as BTK, MAPK1, MAPK3 or phospholipase C-gamma. This cascade promotes the neutrophil- and macrophage-mediated release of proinflammatory cytokines and/or chemokines, as well as their migration and thereby amplifies inflammatory responses that are triggered by bacterial and fungal infections. By also promoting the amplification of inflammatory signals that are initially triggered by Toll-like receptor (TLR) and NOD-like receptor engagement, plays a major role in the pathophysiology of acute and chronic inflammatory diseases of different etiologies including septic shock and atherosclerosis.; Acts as a decoy receptor, counterbalancing TREM1 proinflammatory activity through the neutralization of its lignad.
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基因功能參考文獻:
- These data show that human macrophages infected with human immunodeficiency virus-1 show an increased expression of TREM-1. In parallel, direct exposure to the human immunodeficiency virus-1-related proteins Tat or gp120 induces TREM-1 expression in macrophages and confers anti-apoptotic attributes. PMID: 28181540
- Current data suggest that sTREM-1 might become a useful biomarker for the prediction of neonatal sepsis; however, the significant variation in the used threshold values (that ranged between 77.5 and 1707.35 pg/ml) and the methodological differences across studies limit the value of our findings in clinical practice.[Review] PMID: 29644420
- TREM-1 is a predictive biomarker of in-stent restenosis and an important mediator of cellular inflammation, migration, and proliferation in vascular smooth muscle cells PMID: 29080545
- The present study highlights the role of urine sTREM-1 levels in late-onset sepsis. PMID: 29480083
- TREM-1 links dyslipidemia to inflammation and lipid deposition in atherosclerosis. PMID: 27762264
- serum levels seem higher in newly diagnosed type 1 diabetes patients than in their healthy siblings (not statistically significant) PMID: 27862781
- TREM-1 interventions by means of LP17, LR12 and TREM-1 fusion protein did not ameliorate IR-induced injury. In the human renal transplant cohort, donor and recipient TREM1 gene variant p.Thr25Ser was not associated with DGF, nor with biopsy-proven rejection or death-censored graft failure. We conclude that TREM-1 does not play a major role during experimental renal IR and after kidney transplantation PMID: 27928159
- TREM-1 plays an important role in mediating platelet activation. PMID: 28837205
- Serum sTREM-1 level is significantly higher in diarrhea-predominant irritable bowel syndrome (D-IBS) patients and positively correlates with abdominal pain, which may be initiated by TREM-1-associated macrophage activation, indicating the existence of subclinical inflammation in D-IBS. PMID: 29516326
- TREM-1 overexpression and M1 macrophage polarization are associated with obesity-induced insulin resistance. PMID: 28454543
- TREM-1 may have a role in pathology of craniopharyngioma and Rathke's cleft cyst PMID: 27409178
- low expression level of TREM-1 might be a characteristic for tumor-associated macrophages in lung cancer PMID: 27244892
- The results demonstrate that both commensal and pathogenic oral bacteria activate the TREM-1 pathway, resulting in a proinflammatory TREM-1 activity-dependent increase in proinflammatory cytokine production. PMID: 27448788
- Our findings suggest a novel modulatory role of TREM-1 in the pathogenesis of SLE. sTREM-1 production is a useful diagnostic marker and a molecular target for combination therapy of lupus. PMID: 28089248
- In the premature rupture of membranes group,the NF-kappaB p65 and sTREM-1 levels in maternal blood were significantly higher in women with chorioamnionitis than women without chorioamnionitis (P<.05). PMID: 27521929
- Diagnostic value of sTREM-1, IL-8, PCT, and CRP in febrile neutropenia after autologous stem cell transplantation PMID: 28920169
- findings showed detectable expression of TREM-1 in 100% of samples, urinary tract infection (UTI) and non-UTI groups; levels of sTREM-1 were detected in patients with UTI, but not in non-UTI patients; TREM-1 levels in patients with UTI tend to be higher and are associated with increased neutrophils and cytokine activity induced by bacteria PMID: 28367708
- The minor allele T of rs2234246 of TERM1 protein, was associated with increased sTREM-1 in the discovery population, explaining 33% of its variance, and with increased levels of mRNA. PMID: 28771614
- This study provided evidence that rs6910730G, an intronic variant of TREM1, reduced the ability of human monocytes for Abeta phagocytosis, and this reduction was likely attributed to a decreased monocytic TREM1 expression. PMID: 27670763
- Studies show that bronchoalveolar lavage fluid (BALF) soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) levels do not discriminate between mild-to-moderate asthma and chronic obstructive pulmonary disease (COPD), in contrast, the interleukin 6 (IL-6)/interleukine 13 (IL-13) ratio measured in BALF effectively differentiated these two diseases. PMID: 28791839
- study found that soluble TREM-1 is increased and correlates with the clinical and laboratory findings in Crimean Congo Haemorrhagic Fever, a viral infection characterized by activation of inflammation. PMID: 26877157
- The findings suggest that there are no differences in soluble TREM-1 levels in gingival crevicular fluid between healthy and periodontally diseased elderly adults PMID: 27542376
- TREM-1 was found to be significantly higher in tissues biopsies and blood of subjects with obesity. Greater expression and activity of TREM-1 suggest a possible role in the underlying pathophysiology of obesity and associated comorbidities. PMID: 28111922
- This study indicates that in children with community-acquired pneumonia, sTREM-1, Midregional-proANP, and Midregional-proADM blood levels have poor abilities to differentiate bacterial from viral diseases or to identify severe cases. PMID: 27846213
- Carriage of the TREM1 rs2234237T allele appears to be a risk factor for the development of severe malaria. PMID: 27671831
- TREM-1, HMGB1 and RAGE in the Shoulder Tendon: Dual Mechanisms for Inflammation Based on the Coincidence of Glenohumeral Arthritis PMID: 27792788
- is concluded that both HMGB1 and sTREM-1 are released during the inflammatory response of periodontal tissues and they can promote inflammatory process, which leads to tissue destruction. PMID: 27917624
- The soluble TREM-1 level, core temperature and SAPS II value were the only independent predictors of death after adjustment for potential confounders in sepsis patients admitted to intensive care. PMID: 27729010
- TREM-1 plays a critical role in osteoarthritis development and IL1B induced chondrocyte injury through regulation of NF-kappaB signaling PMID: 27932245
- sTREM1 was increased in septic patients with myocardial depression compared to those without myocardial depression. PMID: 27238916
- this study shows higher levels of TREM-1 in plasma of systemic lupus erythematosus in Egyptian patients PMID: 27648678
- Increased expression of TREM-1 in S compared to AS patients involving TNFa, MMP-1 and MMP-9 suggest a potential role of TREM-1 in plaque destabilization PMID: 27017522
- decreased serum level significantly associated with the presence and severity of coronary artery disease; restrains inflammatory reaction in endothelial cells PMID: 27631216
- Plasma levels of sTREM-1 were found to identify patients with septic shock more effectively than procalcitonin and C-reactive protein. Moreover, sTREM-1 was identified to be an early predictor for survival in patients with septic shock. PMID: 27319606
- This study provides new insights into the possible mechanism of TREM-1 and HIF-1alpha in psoriasis. PMID: 27743451
- TREM-1 may play an important role in the inflammatory cascade after subarachnoid hemorrhage and act as a monitoring biomarker PMID: 27697433
- This study shows increased expression of TREM-1 in monocytes from patients with advanced cervical cancer. PMID: 26506490
- TREM-1 expression is decreased during the generation of human osteoclast precursors PMID: 26852644
- TREM-1 activation may be involved in the development of Kawasaki disease. PMID: 27324541
- LPS-induced TREM-1 transcription. PMID: 26397033
- The study simultaneously evaluated both PCT and soluble TREM-1 along with CRP in febrile patients with autoimmune diseases. PMID: 27096761
- TREM-1 and Dectin-1 function concurrently in the corneal innate immune response by regulating inflammatory cytokine expression in fungal keratitis. PMID: 26963514
- High TREM-1 expression correlated significantly with increased recurrence in hepatocellular carcinoma. PMID: 25465376
- Soluble TREM-1 level is significantly increased in hemodialysis patients as are other pro-inflammatory markers. PMID: 26495896
- TREM-1 is induced by MSU and is associated with the inflammation of human acute gouty arthritis. PMID: 26320130
- Plasma and urine TREM-1 levels can be used as diagnostic biomarkers for acute kidney injury in critically ill patients with sepsis. PMID: 25944130
- study found no association between the other investigated SNPs within TREM-1 gene and infective endocarditis risk PMID: 25213166
- In neonates with late-onset neonatal sepsis, soluble TREM-1 has the potential to provide an excellent predictive value for septic shock/death. PMID: 25461656
- Soluble TREM-1, a member of the immunoglobulin super-family, is upregulated when neutrophils are exposed to bacteria. PMID: 25294884
- isolated PMNs have an increased proportion of both TREM1 and DAP12 compared to normal healthy control PMID: 25642940
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亞細胞定位:[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.
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組織特異性:Mostly expressed by immune cells of the myeloid lineage, such as monocytes, macrophages, neutrophils and dendritic cells. Expression is associated with a mature stage of myeloid development. Highly expressed in adult liver, lung and spleen than in corresp
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