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  4. Human Troponin I, fast skeletal muscle(TNNI2) ELISA kit

Human Troponin I, fast skeletal muscle(TNNI2) ELISA kit

  • 中文名稱：
    人肌鈣蛋白I，骨骼肌快肌(TNNI2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL024012HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人肌鈣蛋白I，骨骼肌快肌(TNNI2)酶聯(lián)免疫試劑盒（CSB-EL024012HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿,組織勻漿樣本中的TNNI2含量。TNNI2 即心肌肌鈣蛋白 I2，它作為心肌收縮調(diào)節(jié)的關(guān)鍵蛋白，與心肌功能緊密相關(guān)。在研究機(jī)制上，其異常表達(dá)或突變會(huì)影響心肌收縮舒張功能，在心肌疾病發(fā)生發(fā)展中起重要作用，是心血管疾病研究的潛在靶點(diǎn)。試劑盒檢測(cè)范圍為6.25 pg/mL-400 pg/mL，適用于肌肉發(fā)育、運(yùn)動(dòng)損傷模型、肌病機(jī)制等基礎(chǔ)研究領(lǐng)域?？蒲腥藛T可通過(guò)定量分析不同生理或病理狀態(tài)下骨骼肌損傷標(biāo)志物TNNI2的動(dòng)態(tài)變化，為探索肌肉再生機(jī)制或藥物干預(yù)效果提供數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    AMCD 2B ELISA Kit; AMCD2B ELISA Kit; DA 2B ELISA Kit; DA2B ELISA Kit; fast skeletal muscle ELISA Kit; Fast twitch skeletal muscle troponin I ELISA Kit; fast-twitch isoform ELISA Kit; FSSV ELISA Kit; fsTnI ELISA Kit; TNNI 2 ELISA Kit; Tnni2 ELISA Kit; TNNI2_HUMAN ELISA Kit; tro ELISA Kit; Troponin I ELISA Kit; Troponin I fast skeletal muscle ELISA Kit; Troponin I fast twitch 2 ELISA Kit; Troponin I fast twitch isoform ELISA Kit; Troponin I fast twitch skeletal muscle isoform ELISA Kit; Troponin I skeletal fast ELISA Kit; Troponin I type 2 (skeletal fast) ELISA Kit; Troponin I type 2 ELISA Kit
  • 縮寫：
    TNNI2
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma,tissue homogenates
  • 檢測(cè)范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human TNNI2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    85

    Range %

    81-90

    1:2

    Average %

    93

    Range %

    88-99

    1:4

    Average %

    92

    Range %

    88-97

    1:8

    Average %

    91

    Range %

    86-96

  • 回收率：

    The recovery of human TNNI2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    92

    87-97

    EDTA plasma (n=4)

    98

    91-104

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    400

    2.236

    2.334

    2.285

    2.127

    200

    1.642

    1.667

    1.655

    1.497

    100

    1.227

    1.288

    1.257

    1.100

    50

    0.857

    0.829

    0.843

    0.686

    25

    0.610

    0.621

    0.616

    0.458

    12.5

    0.429

    0.453

    0.441

    0.283

    6.25

    0.244

    0.234

    0.239

    0.081

    0

    0.160

    0.155

    0.158

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

■ 常見問(wèn)題解答
Q:

Per customer request, can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
c. Standard – if recombinant protein please specify sequence (including species source and accession no. if available) and expression host.
d. The customer is asking about cross-reactivity with homologous proteins, espessialy with cTnI. Did you test it? Can you please provide such kind of information with data?

A:
Thanks for your inquiry!
CSB-EL024012hu
This kit is designed against full length protein.
Capture antibody is goat polyclonal antibody.
Detection antibody is goat polyclonal antibody.
Immunogen of capture antibody is 293 cell derived recombinant protein, full length.
Immunogen of detection antibody is 293 cell derived recombinant protein, partial.
Standard is 293 cell derived recombinant protein.There is no cross reactivity with cTnI.

靶點(diǎn)詳情

  • 功能：
    Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 基因功能參考文獻(xiàn)：
    1. Mutations in genes encoding cardiac troponin I (TNNI3) and cardiac troponin T (TNNT2) caused altered troponin protein stoichiometry in patients with dilated cardiomyopathy. TNNI3p.98trunc resulted in haploinsufficiency, increased Ca(2+) -sensitivity and reduced length-dependent activation. TNNT2p.K217del caused increased passive tension. PMID: 28436080
    2. TNNI2 gene missense mutation is associated with distal arthrogryposis type 1. PMID: 26374086
    3. report the first TNNI2 mutation in classical FSS and describe an atypical adult FSS case with only facial contractures resulting from somatic mosaicism PMID: 23850728
    4. Molecular genetic investigations revealed pathogenic mutations in MYH3, TPM2, and TNNI2 in one sporadic and 19 familial cases of distal arthrogryposis. PMID: 22519952
    5. Atrial re-expression of ssTnI during paroxysmal atrial fibrillation in patients does not seem to be part of the process of AF-associated cardiomyocyte dedifferentiation but seems to result from transient cardiomyocyte stress at the onset of AF. PMID: 15289369
    6. study found a novel TNNI2 mutation in a Chinese family with distal arthrogryposis type 2B PMID: 16802141
    7. Implantation of stable human clone expressing fast-twitch skeletal muscle troponin I in female BALB/c nude mice inhibits primary tumor growth and suggests that grafts are self-inhibitory by halting angiogenesis. PMID: 17393089
    8. These findings reveal a new function for TNNI2 as a co-activator of ERRalpha. PMID: 18331830
    9. Data found that fTnI normally associated with fast twitch skeletal muscle were present at significant levels in the thoracic aorta, and that fTnI transcripts were expressed in the smooth muscle layer of mouse blood vessels of all sizes. PMID: 18548613
    10. Gene expression revealed up-regulation of pro-angiogenic (PGF), anti-apoptotics (BAG-1, BCL-2), heart development (TNNT2, TNNC1) and extracellular matrix remodelling (MMP-2, MMP-7) genes in SM. PMID: 18805052

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  • 相關(guān)疾?。?/div>
    Arthrogryposis, distal, 2B (DA2B)
  • 蛋白家族：
    Troponin I family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 11946

    OMIM: 191043

    KEGG: hsa:7136

    STRING: 9606.ENSP00000252898

    UniGene: Hs.523403