搡老女人多毛老妇女中国,日韩亚洲欧美中文高清在线,人妻少妇一区二区三区,色妞色综合久久夜夜,日本熟妇xxxx

  1. 首頁(yè)
  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Human Tumor necrosis factor-inducible gene 6 protein(TNFAIP6) ELISA kit

Human Tumor necrosis factor-inducible gene 6 protein(TNFAIP6) ELISA kit

  • 中文名稱(chēng)：
    人腫瘤壞死因子誘導(dǎo)基因6蛋白(TNFAIP6)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL023959HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人腫瘤壞死因子誘導(dǎo)基因6蛋白(TNFAIP6)酶聯(lián)免疫試劑盒（CSB-EL023959HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TNFAIP6含量。TNFAIP6 靶點(diǎn)具有重要生物學(xué)意義。它可能參與炎癥、組織修復(fù)等生理過(guò)程。相關(guān)研究聚焦于其在疾病發(fā)生發(fā)展中的作用機(jī)制，探索其作為治療靶點(diǎn)的潛力，如在炎癥性疾病等領(lǐng)域，為開(kāi)發(fā)針對(duì)性藥物提供理論基礎(chǔ)。試劑盒檢測(cè)范圍為47 ng/ml- 3000 ng/ml，適用于基礎(chǔ)科研中探索TNFAIP6在炎癥性疾病、纖維化病變或腫瘤發(fā)展中的表達(dá)規(guī)律，亦可用于評(píng)估體外實(shí)驗(yàn)?zāi)Ｐ椭兴幬锔深A(yù)或基因調(diào)控對(duì)該靶點(diǎn)的影響。為研究TNFAIP6在病理生理過(guò)程中的動(dòng)態(tài)變化提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Hyaluronate binding protein ELISA Kit; Hyaluronate-binding protein ELISA Kit; TNF alpha induced protein 6 ELISA Kit; TNF alpha-induced protein 6 ELISA Kit; TNF stimulated gene 6 protein ELISA Kit; TNF-stimulated gene 6 protein ELISA Kit; TNFAIP 6 ELISA Kit; Tnfaip6 ELISA Kit; TSG 6 ELISA Kit; TSG-6 ELISA Kit; TSG6_HUMAN ELISA Kit; Tumor necrosis factor alpha induced protein 6 ELISA Kit; Tumor necrosis factor alpha inducible protein 6 ELISA Kit; Tumor necrosis factor alpha-induced protein 6 ELISA Kit; Tumor necrosis factor inducible gene 6 protein ELISA Kit; Tumor necrosis factor stimulated gene 6 protein ELISA Kit; Tumor necrosis factor-inducible gene 6 protein ELISA Kit
  • 縮寫(xiě)：
    TNFAIP6
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    47 ng/ml - 3000 ng/ml
  • 靈敏度：
    11.75 ng/ml
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human TNFAIP6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    99

    Range %

    92-106

    1:2

    Average %

    85

    Range %

    81-92

    1:4

    Average %

    89

    Range %

    84-94

    1:8

    Average %

    95

    Range %

    88-101

  • 回收率：

    The recovery of human TNFAIP6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    94

    89-100

    EDTA plasma (n=4)

    88

    81-95

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    3000

    2.715

    2.634

    2.674

    2.546

    1500

    2.032

    2.167

    2.099

    1.972

    750

    1.329

    1.278

    1.303

    1.175

    375

    0.786

    0.749

    0.767

    0.640

    187.5

    0.427

    0.411

    0.419

    0.291

    94

    0.294

    0.278

    0.286

    0.158

    47

    0.227

    0.217

    0.222

    0.094

    0

    0.131

    0.125

    0.128

    　

  • 本試劑盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human TNFAIP6 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled TNFAIP6 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

    顯示更多

    收起更多

  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

    顯示更多

    收起更多

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類(lèi)型：血漿（抗凝劑） EDTA

樣品信息：人

稀釋比：沒(méi)有稀釋

產(chǎn)品評(píng)價(jià)： ELISA結(jié)果正常組和患病組有明顯差異

By 劉老師

靶點(diǎn)詳情

  • 功能：
    Possibly involved in cell-cell and cell-matrix interactions during inflammation and tumorigenesis.
  • 基因功能參考文獻(xiàn)：
    1. TSG-6 up-regulation counteracts the progression of atherosclerosis. (Review) PMID: 29401724
    2. TSG-6 from MSCs attenuated severe burn-induced excessive inflammation via inhibiting activation of P38 and JNK signaling. PMID: 27444207
    3. TSG-6 secreted by human mesenchymal stem cells suppresses inflammatory reactions through p38 and ERK in the mitogen-activated protein kinase pathway PMID: 28247086
    4. This review will focus on the potential of mesenchymal stem/stromal cells for treatment of fibrotic diseases, with emphasis on the role of TSG-6 as a mediator of anti-inflammatory effects PMID: 26807758
    5. abnormally elevated in both the plasma and aortic wall of patients with abdominal aortic aneurysms PMID: 29180197
    6. Single nucleotide polymorphism in TNFAIP6 gene is associated with Systemic lupus erythematosus. PMID: 28246883
    7. TNFAIP6 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
    8. Data show that TNF-stimulated gene-6 (TSG-6) interacts with chemokines through their glycosaminoglycans (GAGs)-binding sites and inhibits their binding to GAGs and endothelial cell surfaces. PMID: 27044744
    9. Data postulate that the molecular cross-linking enhanced by the multiple binding modes of the Link module might be critical for remodeling the ECM during inflammation/ovulation and might contribute to other functions of TSG-6. PMID: 26685054
    10. the well characterized hyaluronan-binding site in the TSG-6 Link module is not used for recognition during transfer of HCs onto HA. PMID: 26468290
    11. TSG-6 exhibited anti-inflammatory effects during the wound healing process and cicatrization and significantly diminished hypertrophic scar formation in a rabbit ear model PMID: 25661977
    12. results suggest that systemic administration of hASC or TSG-6 may be novel approaches to reverse CS-induced myelosuppression. PMID: 25329668
    13. TSG-6 activity in tissues, was examined. PMID: 25325979
    14. TSG-6 expression by hMSCs was induced 12 h after oropharyngeal delivery to LPS-exposed lungs. Knockdown of TSG-6 expression in hMSCs by RNA interference abrogated most of their anti-inflammatory effects. PMID: 21569482
    15. Heavy chain transfer by tumor necrosis factor-stimulated gene 6 to the bikunin proteoglycan. PMID: 25561734
    16. data suggest that TSG-6 expression might be essential in endometrial matrix organization and feto-maternal communication during the implantation process. PMID: 24213015
    17. the HA-binding site defined here may not play a role in TSG-6-mediated transfer of heavy chains from inter-alpha-inhibitor onto HA, a process known to be essential for ovulation. PMID: 24403066
    18. TSG-6 suppresses CXCL8-mediated chemotaxis of neutrophils; this lower potency effect might be important at sites where there is high local expression of TSG-6. PMID: 24501198
    19. This study provides insight into what we believe to be a previously undescribed multifaceted role of mesenchymal stem cell-released TSG-6 in wound healing. PMID: 23921952
    20. Data indicate that TSG-6-mediated cross-linking of hyaluronan (HA) films is impaired in the presence of inter-alpha-inhibitor (IalphaI) and that this effect suppresses the TSG-6-mediated enhancement of HA binding to CD44-positive cells. PMID: 24005673
    21. The protein product of tumor necrosis factor-alpha stimulated gene-6 strongly correlated with ossification markers and hyaluronidase in calcified human aortic valves. PMID: 23017666
    22. This review discusses TSG-6 as a potential molecular marker of oocyte maturation. PMID: 22922154
    23. TSG-6 increases the accumulation of HA in the cell-associated matrix, partially by preventing its dissolution from the cell-associated matrix into the conditioned medium, but primarily by inducing HA synthesis PMID: 23129777
    24. analysis of irreversible heavy chain transfer to hyaluronan oligosaccharides by tumor necrosis factor-stimulated gene-6 PMID: 23166324
    25. TSG-6 reverts the inhibitory effects exerted by PTX3 on FGF2-mediated angiogenesis through competition of FGF2/PTX3 interaction. PMID: 22267482
    26. TSG-6 was central to EMT through effects on HA macromolecular structure and through CD44-dependent triggering of cell responses. PMID: 21864707
    27. TSG-6 is a potent HA cross-linking agent PMID: 21596748
    28. TSG-6 protein, a negative regulator of inflammatory arthritis, forms a ternary complex with murine mast cell tryptases and heparin. PMID: 21566135
    29. negative correlation between TSG-6 expression levels and severity of capsular contracture (CC) suggests a possible protective role for TSG-6 in the context of CC formation, and this may have a clinically relevant role in prevention of breast CC PMID: 21239672
    30. TSG-6/HC2 link heavy chains randomly on the chondroitin sulfate chain of bikunin, in contrast to the ordered attachment observed during the biosynthesis. PMID: 20463016
    31. polymorphism in osteoarthritis; chromosome location given here (chromosome 2) PMID: 11854277
    32. TSG-6 is a multifunctional protein associated with inflammation [review] PMID: 12692188
    33. Tumor necrosis factor, alpha-induced protein 6 activates an anti-inflammatory agent- inter-alpha-inhibitor[review]. PMID: 14515153
    34. The TSG-6 and I alpha I interaction promotes a transesterification cleaving the protein-glycosaminoglycan-protein (PGP) cross-link. PMID: 15653696
    35. This TSG-6 model reveals the residues most likely to be involved in stabilizing the inter-Link module interface, which are highly conserved across the Link protein/ chondroitin sulfate proteoglycan subfamily. PMID: 15718240
    36. TSG-6 treatment also resulted in a rapid increase in cyclooxygenase-2 (COX-2) mRNA levels, suggesting that TSG-6 up-regulates COX-2 gene expression PMID: 15809059
    37. TSG-6 acts as cofactor and catalyst in the production of IalphaI heavy chain x hyaluronan complexes PMID: 15840581
    38. heparin with the Link module significantly increases the anti-plasmin activity of the TSG-6.IalphaI complex; TSG-6 is likely to contribute to matrix remodeling, at least in part, through down-regulation of the protease network PMID: 15917224
    39. The tumor necrosis factor alpha induced protein 6 is a potential marker that could be used for early diagnosis and prognosis of cancerous or precancerous lesions. PMID: 16467113
    40. hyaluronan synthase 2 and TSG-6 have roles in hyaluronan distribution and function in proximal tubular epithelial cells PMID: 16687630
    41. Evidence is cited in this review that TSG-6 can regulate the expression of various molecules that have important roles in the control of inflammation. PMID: 16709183
    42. TSG-6 is down-regulated during osteoblastic differentiation, and suppressed osteoblastic differentiation induced by both BMP-2 and osteogenic differentiation medium. PMID: 16771708
    43. TSG-6 may play an important protective role in bronchial epithelium by increasing the antiprotease screen on the airway lumen. PMID: 16873769
    44. Crystallography data on the free protein, and (15)N NMR relaxation data for the uncomplexed and HA(8)-bound forms of Link_TSG6, is presented. PMID: 17585936
    45. TSG-6 is a high affinity ligand that can mediate fibronectin interactions with other matrix components and modulate some interactions of fibronectin with cells PMID: 18042364
    46. 3 genes were upregulated in patients with chronic EBV infection: guanylate binding protein 1, tumor necrosis factor-induced protein 6, and guanylate binding protein 5; they may be associated with the inflammatory reaction or with cell proliferation. PMID: 18260761
    47. The transfer of heavy chains from bikunin proteins to hyaluronan requires both TSG-6 and HC2. PMID: 18448433
    48. TSG-6 has dual roles in bone remodeling: it inhibits RANKL-induced bone erosion in inflammatory diseases, and its interactions with BMP-2 and RANKL help to balance mineralization by osteoblasts and bone resorption by osteoclasts PMID: 18586671
    49. TSG-6 transfers proteins between glycosaminoglycans via a Ser28-mediated covalent catalytic mechanism PMID: 18820257
    50. myelomonocytic cells and MoDC are a major source of TSG-6 and that PTX3 and TSG-6 are coregulated PMID: 19389798

    顯示更多

    收起更多

  • 組織特異性：
    Found in the synovial fluid of patients with rheumatoid arthritis.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 11898

    OMIM: 600410

    KEGG: hsa:7130

    STRING: 9606.ENSP00000243347

    UniGene: Hs.437322