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  4. Human Zinc finger protein Aiolos(IKZF3) ELISA kit

Human Zinc finger protein Aiolos(IKZF3) ELISA kit

  • 中文名稱：
    人鋅指蛋白Aiolos(IKZF3)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL011577HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人鋅指蛋白Aiolos(IKZF3)酶聯(lián)免疫試劑盒（CSB-EL011577HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的IKZF3含量。IKZF3 是重要靶點，它屬于 IKAROS 轉(zhuǎn)錄因子家族。在正常生理中對淋巴細胞發(fā)育有調(diào)控作用。研究發(fā)現(xiàn)其異常表達與多種血液系統(tǒng)惡性腫瘤相關(guān)，如多發(fā)性骨髓瘤等，機制可能涉及對腫瘤細胞增殖、存活、凋亡等過程的影響。試劑盒檢測范圍為25 pg/mL-1600 pg/mL，本試劑盒采用高特異性配對抗體，可精準捕獲目標蛋白，適用于科研領(lǐng)域?qū)γ庖哒{(diào)控機制、腫瘤微環(huán)境研究及藥物干預(yù)效果評估等方向，為探索IKZF3在免疫應(yīng)答、腫瘤免疫逃逸中的分子機制提供可靠工具。實驗操作全程可在常規(guī)實驗室條件下完成，兼容96孔板檢測系統(tǒng)，支持批量樣本分析，滿足基礎(chǔ)研究與轉(zhuǎn)化醫(yī)學(xué)中對生物標志物定量檢測的需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    AIO ELISA Kit; Aiolos ELISA Kit; IKAROS family zinc finger 3 (Aiolos) ELISA Kit; IKAROS family zinc finger 3 ELISA Kit; Ikaros family zinc finger protein 3 ELISA Kit; IKZF 3 ELISA Kit; IKZF3 ELISA Kit; IKZF3_HUMAN ELISA Kit; zinc finger DNA binding protein Aiolos ELISA Kit; Zinc finger protein Aiolos ELISA Kit; Zinc finger protein subfamily 1A 3 (Aiolos) ELISA Kit; Zinc finger protein subfamily 1A 3 ELISA Kit; Zinc finger protein subfamily 1A; member 3 ELISA Kit; ZNFN1A3 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    25 pg/mL-1600 pg/mL
  • 靈敏度：
    6.25 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IKZF3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-91
    1:2Average %96
    Range %91-104
    1:4Average %96
    Range %92-109
    1:8Average %94
    Range %86-97
  • 回收率：
    The recovery of human IKZF3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-97
    EDTA plasma (n=4)9690-99
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    16002.098 1.986 2.042 1.950
    8001.357 1.298 1.328 1.236
    4000.825 0.789 0.807 0.715
    2000.500 0.481 0.491 0.399
    1000.360 0.351 0.356 0.264
    500.245 0.240 0.243 0.151
    250.156 0.152 0.154 0.062
    00.092 0.091 0.092 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.
  • 基因功能參考文獻：
    1. The minor alleles of rs2941522, rs907091, rs1453559, rs12150079 and rs2872507 were statistically associated with an increased risk in Graves' disease patients. PMID: 29510406
    2. Aiolos promotes the binding of Blimp-1 to target genes and thereby enhances Blimp-1-dependent transcriptional repression. PMID: 26823144
    3. Our data indicate a possible regulatory role for the multiple sclerosis-associated IKZF3 and IQGAP1 variants. PMID: 27080863
    4. results indicate that overexpression of Aiolos may contribute to pathogenesis of SLE and RA PMID: 26546109
    5. Aiolos improved the survival of Nalm6 cells via PTEN and Aktdependent processes. PMID: 25608224
    6. NFKB1, CD27, LAG3 and IKZF3 are new susceptibility genes for psoriasis. PMID: 25006012
    7. lentivirus-mediated AIOLOS overexpression in Jurkat cells induced cell apoptosis, arrested the cell cycle at the G0/G1 phase, and synergistically increased the sensitivity of Jurkat cells to etoposide by inhibiting NF-kappaB activity. PMID: 25524659
    8. identified a unique case of adult acute B-cell lymphoblastic leukemia with masked low hypodiploidy (mLH) by genomic duplication PMID: 26185311
    9. The allele T of rs10852936 surrounding the IKZF3-ZPBP2 locus confers risk for early-onset psoriasis. PMID: 25620289
    10. A strong association between rs907091 in the IKZF3 gene and SLE was identified. PMID: 25271777
    11. The immunosuppressive enzyme IL4I1 expressed differentially in human induced Aiolos+, but not natural Helios+, FOXP3+ Treg cells. PMID: 25446972
    12. A strong allelic association between SNPs in the 17q21 region (IKZF3) and asthma has been confirmed for the Latinos population. PMID: 24406073
    13. Overexpression of AIOLOS inhibits cell proliferation, suppresses apoptosis and arrests the cell cycle at the G0/G1 phase in vitro. PMID: 24399134
    14. Aiolos reconfigures chromatin structure within the SHC1 gene, causing isoform-specific silencing of the anchorage reporter p66(Shc) and blocking anoikis in vitro and in vivo. PMID: 24823637
    15. Sixteen SNPs were significantly associated with asthma of which one SNP was novel (IKZF3-rs1453559). PMID: 22626592
    16. Four additional susceptibility loci (IRF8, TMEM39A, IKZF3, and ZPBP2) for systemic lupus erythematosus were robustly established a multiethnic population (European, African American, Asian, Hispanic, Gullah, and Amerindian). PMID: 22464253
    17. To determine the consequences of Aiolos deregulation in B-CLL, we analyzed the effects of Aiolos overexpression or down-regulation on apoptosis. PMID: 21139082
    18. distribution of positive and negative expression of Aiolos and Helios found in various types of leukemias could implicate common pathways of their regulation PMID: 20432734
    19. Aiolos knockout mice develop the symptoms of human systemic lupus erythematosus (SLE). This finding implicates Aiolos as a possible candidate gene for SLE. PMID: 12574333
    20. we show for the first time the differential Aiolos expression at the RNA and protein level in hematopoietic cell subpopulations. PMID: 19540588
    21. Short isoforms of Aiolos were detected at low levels in bone marrow of normal volunters, but were not overexpressed in a patient with acute T-cell leukemia. Data for unaltered Aiolos indicates tumor suppressor function. PMID: 11937265
    22. impairment of Aiolos activity in human leukemia is not based on deficient phosphorylation as had been demonstrated in experiments in vitro PMID: 17296582
    23. this work demonstrates that the cellular activities of Aiolos isoforms are dependent on combinations of various functional domains arising from the differential splicing of mRNA transcripts PMID: 17646674
    24. up-regulation of Aiolos, confirmed at protein level, seems independent of Aiolos promoter H3K9 acetylation and H3K4 trimethylation PMID: 18184862
    25. DNA methylation directs Aiolos silencing and chromatin status in tumor cell lines, while in primary cells is mainly regulated by histone modifications. PMID: 18206652

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  • 亞細胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Ikaros C2H2-type zinc-finger protein family
  • 組織特異性：
    Expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 13178

    OMIM: 606221

    KEGG: hsa:22806

    STRING: 9606.ENSP00000344544

    UniGene: Hs.371680