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  4. Human alpha-L-fucosidase,AFU ELISA Kit

Human alpha-L-fucosidase,AFU ELISA Kit

  • 中文名稱：
    人αL巖藻糖苷酶(AFU)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08348h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人αL巖藻糖苷酶(AFU)酶聯(lián)免疫試劑盒（CSB-E08348h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FUCA2含量。FUCA2 是一個靶點。其相關它在機體特定生理過程中可能發(fā)揮重要作用。研究機制上，科學家可能會從基因表達調(diào)控、蛋白功能等角度展開研究，探究其在疾病發(fā)生發(fā)展中的作用，以尋找潛在治療靶點和干預措施。試劑盒檢測范圍為31.25 μU/mL-2000 μU/mL，適用于體外科研實驗中AFU的精準定量分析；可為肝臟病理機制研究、腫瘤標志物篩選、代謝性疾病模型構建等基礎科研提供可靠數(shù)據(jù)支持；適用于實驗室批量檢測需求本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Alpha L fucosidase 2 ELISA Kit; Alpha L fucosidase fucohydrolase 2 ELISA Kit; Alpha-L-fucosidase 2 ELISA Kit; Alpha-L-fucoside fucohydrolase 2 ELISA Kit; dJ20N2.5 ELISA Kit; FUCA 2 ELISA Kit; FUCA2 ELISA Kit; FUCO2 ELISA Kit; FUCO2_HUMAN ELISA Kit; Fucosidase alpha L 2 plasma ELISA Kit; Fucosidase; alpha-L; plasma ELISA Kit; MGC1314 ELISA Kit; Plasma alpha L fucosidase ELISA Kit; Plasma alpha-L-fucosidase ELISA Kit; RP1 20N2.5 ELISA Kit
  • 縮寫：
    FUCA2
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    31.25 μU/mL-2000 μU/mL
  • 靈敏度：
    7.8 μU/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human AFU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:20Average %88
    Range %81-95
    1:40Average %91
    Range %86-97
    1:80Average %97
    Range %91-102
    1:160Average %102
    Range %97-107
  • 回收率：
    The recovery of human AFU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10395-110
    EDTA plasma (n=4)9385-98
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    µU/mlOD1OD2AverageCorrected
    20002.720 2.546 2.633 2.546
    10002.235 2.200 2.218 2.131
    5001.554 1.633 1.594 1.507
    2500.821 0.845 0.833 0.746
    1250.471 0.445 0.458 0.371
    62.50.311 0.302 0.307 0.220
    31.250.185 0.188 0.187 0.100
    00.084 0.090 0.087 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.
  • 基因功能參考文獻：
    1. Genetic polymorphisms within the FUCA2 and IL18 gene regions are also associated with diastolic function in SCD, likely by affecting expression levels of the genes PMID: 27636371
    2. serum alpha-L-fucosidase was identified as a useful marker for close monitoring of patients during post-treatment follow-up PMID: 18521898
    3. Findings not only demonstrate an important connection between FUCA2 and the adhesion, growth, and pathogenicity of H. pylori, but also support the idea that FUCA2 is a potential target for diagnosis and therapy of H. pylori-related diseases. PMID: 19666478
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Glycosyl hydrolase 29 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 4008

    OMIM: 136820

    KEGG: hsa:2519

    STRING: 9606.ENSP00000002165

    UniGene: Hs.17680