Human angiostatin,ANG ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人血管抑素/血管穩(wěn)定蛋白(ANG)酶聯(lián)免疫試劑盒（CSB-E09195h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、組織培養(yǎng)上清液樣本中的ANG含量。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，適用于體外研究ANG在心血管疾病、腫瘤血管生成、傷口愈合等領(lǐng)域的表達調(diào)控機制，或用于評估藥物干預(yù)對血管生成相關(guān)通路的影響。在腫瘤微環(huán)境、缺血性疾病及炎癥相關(guān)研究中具有重要價值。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

ANG
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell culture supernates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of Human angiostatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	98
	Range %	94-102
1:40	Average %	92
	Range %	86-96
1:80	Average %	94
	Range %	89-98
1:160	Average %	96
	Range %	92-100

回收率：

The recovery of Human angiostatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-98
EDTA plasma (n=4)	88	82-94

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.