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  4. Human angiotensin Ⅰ,Ang-Ⅰ ELISA Kit

Human angiotensin Ⅰ,Ang-Ⅰ ELISA Kit

  • 中文名稱：
    人血管緊張素Ⅰ(Ang-Ⅰ)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08527h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人血管緊張素Ⅰ(Ang-Ⅰ)酶聯(lián)免疫試劑盒（CSB-E08527h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液樣本中的Ang-Ⅰ含量。Ang-Ⅰ，即血管生成素-1（ANG-1），是一種分泌型糖蛋白，屬于血管生成素家族。它作為酪氨酸激酶受體Tie-2的配體，主要作用于血管系統(tǒng)，控制血管的發(fā)育和穩(wěn)定。Ang-Ⅰ在血管成熟過程中起重要作用，介導(dǎo)內(nèi)皮細(xì)胞的遷移、粘附和存活，同時影響骨骼肌細(xì)胞并協(xié)調(diào)血管對組織損傷的反應(yīng)。研究顯示，Ang-Ⅰ在腫瘤生長中發(fā)揮關(guān)鍵作用，可促進(jìn)腫瘤血管生成和腫瘤細(xì)胞增殖，抑制凋亡。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，該產(chǎn)品可廣泛應(yīng)用于體外模型研究、藥物干預(yù)實驗及病理生理學(xué)分析等領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    angiotensin Ⅰ,Ang-Ⅰ,angiotensin 1,Ang-1
  • 縮寫：
    Ang-Ⅰ
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Ang-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %100
    Range %97-105
    1:2Average %98
    Range %94-103
    1:4Average %105
    Range %100-110
    1:8Average %108
    Range %102-114
  • 回收率：
    The recovery of human Ang-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-97
    EDTA plasma (n=4)9590-101
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.683 2.752 2.718 2.567
    5001.905 1.989 1.947 1.796
    2501.037 1.122 1.080 0.929
    1250.631 0.655 0.643 0.492
    62.50.431 0.465 0.448 0.297
    31.20.307 0.321 0.314 0.163
    15.60.230 0.224 0.227 0.076
    00.147 0.154 0.151 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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