Human angiotensin Ⅱ receptor 1 Antibody,ATⅡR1 Ab ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人血管緊張素Ⅱ受體1抗體(ATⅡR1)酶聯(lián)免疫試劑盒（CSB-E10374h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的ATⅡR1 Ab含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于心血管疾病機(jī)制研究、藥物干預(yù)效果評(píng)估或相關(guān)動(dòng)物模型中的靶標(biāo)分子水平分析，為探索ATⅡR1在體液及組織中的表達(dá)規(guī)律提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

angiotensin 2 receptor 1 antibody, AT2R1-Ab
縮寫：

ATⅡR1 Ab
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ATⅡR1 antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	87-105
1:2	Average %	94
	Range %	84-100
1:4	Average %	98
	Range %	92-101
1:8	Average %	94
	Range %	82-105

回收率：

The recovery of human ATⅡR1 antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	83-105
EDTA plasma (n=4)	95	85-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.