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  1. 首頁(yè)
  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Human anti-Granulocyte-Macrophage Colony Stimulating Factor(GM-CSF) antibody ELISA Kit

Human anti-Granulocyte-Macrophage Colony Stimulating Factor(GM-CSF) antibody ELISA Kit

  • 中文名稱(chēng)：
    人粒細(xì)胞巨噬細(xì)胞集落刺激因子（GM-CSF)抗體(酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E11438h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人粒細(xì)胞巨噬細(xì)胞集落刺激因子（GM-CSF)抗體(酶聯(lián)免疫試劑盒（CSB-E11438h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清樣本中的GM-CSF Ab含量。GM-CSF抗體是一種針對(duì)粒細(xì)胞-巨噬細(xì)胞集落刺激因子（GM-CSF）的抗體，GM-CSF是一種重要的免疫調(diào)節(jié)因子，參與免疫細(xì)胞增殖和分化。GM-CSF抗體通過(guò)阻斷GM-CSF與其受體的結(jié)合，抑制GM-CSF的活性，從而調(diào)節(jié)免疫反應(yīng)，用于治療某些炎癥和自身免疫性疾病。研究機(jī)制主要涉及GM-CSF信號(hào)通路和免疫調(diào)節(jié)過(guò)程。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL，科研人員可將其應(yīng)用于探索GM-CSF在疾病發(fā)生發(fā)展中的生物學(xué)機(jī)制、評(píng)估藥物干預(yù)對(duì)細(xì)胞因子網(wǎng)絡(luò)的影響，或作為免疫相關(guān)動(dòng)物模型研究的配套檢測(cè)工具，為免疫學(xué)、腫瘤學(xué)及炎癥性疾病領(lǐng)域的基礎(chǔ)研究提供有效的實(shí)驗(yàn)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    GM-CSF Ab
  • 種屬：
    Homo sapiens (Human)
  • 樣本類(lèi)型：
    serum
  • 檢測(cè)范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線(xiàn)性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human GM-CSF antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 85-101  
    1:2 Average % 96  
    Range % 84-100  
    1:4 Average % 96  
    Range % 90-103  
    1:8 Average % 95  
    Range % 82-106  
  • 回收率：
    The recovery of human GM-CSF antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 86-101  
                 
                 
  • 標(biāo)準(zhǔn)曲線(xiàn)：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    40 2.597 2.648 2.623 2.518  
    20 2.123 2.131 2.127 2.022  
    10 1.466 1.472 1.469 1.364  
    5 0.831 0.896 0.864 0.759  
    2.5 0.472 0.494 0.483 0.378  
    1.25 0.312 0.297 0.305 0.200  
    0.625 0.225 0.241 0.233 0.128  
    0 0.102 0.107 0.105 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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