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  3. 酶聯(lián)免疫試劑盒
  4. Human anti-Survivin(Surv) antibody ELISA Kit

Human anti-Survivin(Surv) antibody ELISA Kit

  • 中文名稱：
    人存活素(Surv)抗體酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E11874h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人存活素(Surv)抗體酶聯(lián)免疫試劑盒（CSB-E11874h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的Surv Ab含量。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL，適用于探索該靶點(diǎn)在細(xì)胞凋亡調(diào)控與腫瘤發(fā)生機(jī)制中的科研應(yīng)用?？蒲腥藛T可將其用于體外培養(yǎng)細(xì)胞上清液、實(shí)驗(yàn)動(dòng)物模型血清或臨床前研究血漿樣本中Survivin蛋白表達(dá)水平的動(dòng)態(tài)監(jiān)測(cè)，為腫瘤發(fā)生機(jī)制解析、藥物敏感性評(píng)估及治療方案優(yōu)化提供數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 縮寫：
    Surv Ab
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma
  • 檢測(cè)范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human anti-surv antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 85  
    Range % 81-90  
    1:2 Average % 96  
    Range % 90-105  
    1:4 Average % 86  
    Range % 81-90  
    1:8 Average % 93  
    Range % 85-100  
  • 回收率：
    The recovery of human anti-surv antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-101  
    EDTA plasma (n=4) 87 82-92  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    40 2.213 2.157 2.185 2.014  
    20 1.612 1.512 1.562 1.391  
    10 1.156 1.113 1.135 0.964  
    5 0.779 0.735 0.757 0.586  
    2.5 0.522 0.492 0.507 0.336  
    1.25 0.383 0.366 0.375 0.204  
    0.625 0.267 0.283 0.275 0.104  
    0 0.168 0.173 0.171 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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