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Human anti-cyclic citrullinated peptide antibody (anti-CCP antibody) ELISA Kit

  • 中文名稱:
    人抗環(huán)胍氨酸肽(CCP)抗體酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E09077h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3200/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人抗環(huán)胍氨酸肽(CCP)抗體酶聯(lián)免疫試劑盒(CSB-E09077h)檢測(cè)血清樣本中的ACCPA含量。人抗環(huán)胍氨酸肽(CCP)抗體是類風(fēng)濕關(guān)節(jié)炎(RA)的重要血清學(xué)標(biāo)志,其檢測(cè)對(duì)RA的早期診斷具有高度特異性。CCP抗體通過(guò)識(shí)別瓜氨酸化抗原表位,在RA發(fā)病機(jī)制中扮演關(guān)鍵角色,其研究有助于深入理解RA的免疫病理過(guò)程。該產(chǎn)品適用于科研場(chǎng)景中抗CCP抗體的體外分析,如類風(fēng)濕關(guān)節(jié)炎發(fā)病機(jī)制研究、藥物干預(yù)效果評(píng)估或與其他生物標(biāo)志物的相關(guān)性分析,為免疫學(xué)研究提供高特異性的檢測(cè)工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 縮寫:
    ACCPA
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum
  • 檢測(cè)范圍:
    Request Information
  • 靈敏度:
    Request Information
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Autoimmunity
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<15%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<15%

    Three samples of known concentration were tested in twenty assays to assess.

  • 標(biāo)準(zhǔn)曲線:

    Test parameter

    specification

    test result

    Positive control

    ≥0.8

    2.078

    Negative control

    ≤0.1

    0.075

    Positive rate

    10,Positive

    100%

    Negative rate

    10,Negative

    100%

  • 本試劑盒所含材料:
    • A 96-well Assay plate --The 96-well plate has been pre-coated with CCP antigen.
    • Negative Control (1 x 0.8 ml)
    • Positive Control (1 x 0.8 ml)
    • HRP-conjugated IgG antibody(1 x 10m)l --Bind to the human anti-CCP antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
    • Sample Diluent (2 x 20 ml) --Dilute the sample to an appropriate concentration.
    • Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
    • Substrate A (1 x 5 ml) --Mix with substrate B and interact with HRP, eliciting a Chromogenic reaction.
    • Substrate B (1 x 5 ml) --Mix with substrate A and interact with HRP, eliciting a Chromogenic reaction.
    • Stop Solution (1 x 5ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual

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  • 本試劑盒不含材料:
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

 常見(jiàn)問(wèn)題解答
Q:

I would like to order Human ELISA kit for anti-CCP3.

A:
Thanks for your inquiry.
Regarding your request,we have this kit for you:#CSB-E09077h,Human anti-cyclic citrullinated peptide antibody (anti-CCP antibody) ELISA Kit. I will ask our distributor in your area to send you a quote by email.
Q:

I found out that that it was a qualitative kit. Is there any way for me to obtain the concentration of the positive control?
I can only get the volume from the kit and not the concentration. Would like to have the concentration so that i can use it for quantitative analysis later on.

A:
Thanks for your inquiry.
Yes this kit is for qualitative determination.Thus you can not obtain the concentration.It can not be used for quantitative analysis.