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  4. Human apelin 13,AP13 ELISA Kit

Human apelin 13,AP13 ELISA Kit

  • 中文名稱：
    人apelin 13(AP13)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13072h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人apelin 13(AP13)酶聯(lián)免疫試劑盒（CSB-E13072h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的AP13含量。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于體外實驗中探索AP13在疾病模型中的表達調(diào)控、藥物干預效果評估或信號通路研究；為心血管與代謝性疾病的基礎研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    AP13
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human AP13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    104

    Range %

    100-108

    1:2

    Average %

    98

    Range %

    91-105

    1:4

    Average %

    101

    Range %

    94-108

    1:8

    Average %

    94

    Range %

    89-100

  • 回收率：

    The recovery of human AP13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    93

    88-99

    EDTA plasma (n=4)

    100

    96-105

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    400

    2.354

    2.434

    2.394

    2.316

    200

    1.326

    1.337

    1.332

    1.253

    100

    0.798

    0.818

    0.808

    0.730

    50

    0.502

    0.529

    0.516

    0.437

    25

    0.249

    0.251

    0.250

    0.172

    12.5

    0.147

    0.153

    0.150

    0.072

    6.25

    0.123

    0.118

    0.121

    0.042

    0

    0.076

    0.081

    0.078

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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