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  4. Human calcitonin gene related peptide,CGRP ELISA Kit

Human calcitonin gene related peptide,CGRP ELISA Kit

  • 中文名稱：
    人降鈣素基因相關(guān)肽(CGRP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08210h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人降鈣素基因相關(guān)肽(CGRP)酶聯(lián)免疫試劑盒（CSB-E08210h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的CGRP含量。CGRP（降鈣素基因相關(guān)肽）是一種神經(jīng)肽。在它在偏頭痛等疾病發(fā)病機(jī)制中扮演重要角色。研究機(jī)制上，它能擴(kuò)張腦血管、調(diào)節(jié)神經(jīng)炎癥。針對 CGRP 及其受體開發(fā)藥物以阻斷其作用，成為偏頭痛等治療的新策略。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，適用于體外研究CGRP在神經(jīng)生物學(xué)、心血管功能、疼痛機(jī)制或炎癥模型中的動態(tài)變化，例如評估實(shí)驗(yàn)動物模型中CGRP的釋放水平、細(xì)胞因子刺激下培養(yǎng)細(xì)胞的分泌特征，或藥物干預(yù)對組織內(nèi)CGRP表達(dá)的影響等科研用途本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Beta type CGRP ELISA Kit; Beta-CGRP ELISA Kit; Beta-type CGRP ELISA Kit; CALC2 ELISA Kit; Calcb ELISA Kit; CALCB_HUMAN ELISA Kit; Calcitonin gene related peptide 2 ELISA Kit; Calcitonin gene related peptide II ELISA Kit; Calcitonin gene-related peptide 2 ELISA Kit; Calcitonin gene-related peptide II ELISA Kit; Calcitonin related polypeptide; beta ELISA Kit; CGRP II ELISA Kit; CGRP-II ELISA Kit; CGRP2 ELISA Kit; CGRPII ELISA Kit; FLJ30166 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    1.56 pg/mL-100 pg/mL
  • 靈敏度：
    0.39 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %80-92
    1:2Average %96
    Range %91-105
    1:4Average %97
    Range %92-110
    1:8Average %90
    Range %86-98
  • 回收率：
    The recovery of human CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9690-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.660 2.511 2.586 2.432
    502.080 2.152 2.116 1.962
    251.451 1.366 1.409 1.255
    12.50.795 0.744 0.770 0.616
    6.250.491 0.523 0.507 0.353
    3.120.315 0.325 0.320 0.166
    1.560.248 0.244 0.246 0.092
    00.157 0.151 0.154 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

相關(guān)問答

■ 常見問題解答
Q:

關(guān)于人降鈣素基因相關(guān)肽CGRP ELISA試劑盒（CSB-E08210h），這個CUSABIO的CGRP Sandwich ELISA檢測的具體是什么？全長還是片段？這個試驗(yàn)?zāi)芊駲z測其他CGRP相關(guān)肽？

A:
CSB-E08210h 檢測的是Beta-type CGRP，參考https://www.uniprot.org/uniprot/P10092針對全長設(shè)計(jì)，至于客戶提到的other CGRP related peptides理論是檢測不了的。如果您有具體信息，可以發(fā)送給我們進(jìn)一步嘗試。

靶點(diǎn)詳情

  • 功能：
    CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.
  • 基因功能參考文獻(xiàn)：
    1. The results suggest that C12orf39, CSTA, and CALCB are novel ATF4 target genes, and that C12orf39 promoter activity is activated by ATF4 through amino acid response element. PMID: 26967115
    2. Keratinocyte-derived CGRPbeta may modulate epidermal homeostasis through autocrine/paracrine signaling and may contribute to chronic pain under pathological conditions. PMID: 21641113
    3. Calcitonin gene-related peptide stimulates proliferation of alveolar epithelial cells. PMID: 19192276
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Calcitonin family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 1438

    OMIM: 114160

    KEGG: hsa:797

    STRING: 9606.ENSP00000346017

    UniGene: Hs.534305