Human carbohydrate-deficient transferrin,CDT ELISA Kit
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中文名稱:人糖缺失性轉(zhuǎn)鐵蛋白(CDT)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E09602h
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規(guī)格:96T/48T
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價(jià)格:¥3200/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人糖缺失性轉(zhuǎn)鐵蛋白(CDT)酶聯(lián)免疫試劑盒(CSB-E09602h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的CDT含量。試劑盒檢測(cè)范圍為50 ng/mL-1600 ng/mL,適用于科研場(chǎng)景中評(píng)估酒精攝入對(duì)轉(zhuǎn)鐵蛋白糖基化修飾的影響,探究慢性酒精暴露導(dǎo)致的肝臟代謝異常機(jī)制,或作為酒精相關(guān)疾病動(dòng)物模型的研究工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
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別名:N/A
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縮寫:CDT
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:50 ng/mL-1600 ng/mL
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靈敏度:25 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Cell Biology
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human CDT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 99 Range % 93-105 1:2 Average % 86 Range % 82-94 1:4 Average % 96 Range % 92-100 1:8 Average % 88 Range % 82-96 -
回收率:
The recovery of human CDT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 91-102 EDTA plasma (n=4) 87 82-93 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 1600 1.849 1.951 1.900 1.865 800 0.983 0.999 0.991 0.956 350 0.476 0.457 0.467 0.432 150 0.264 0.247 0.256 0.221 50 0.104 0.108 0.106 0.071 0 0.034 0.036 0.035 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Does ethyl glucuronide in hair correlates with alcohol consumption? A comparative study with other traditional biomarkers among individuals with alcohol dependence syndrome S Ghosh,Alcohol,2022
- Use of filter paper to measure alcohol biomarkers among opioid-dependent patients on agonist maintenance treatment: A community-based study Quraishi R, et al,Indian Journal of Psychological Medicine,2019
- Carbohydrate Deficient Transferrin and Interleukin-6 as Predictors of Fibrosis in Alcohol Cirrhosis Hanumanthappa Nandeesha. et al,Indian Journal of Clinical Biochemistry,2015
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