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  4. Human casein alpha s1 (CSN1S1) ELISA kit

Human casein alpha s1 (CSN1S1) ELISA kit

  • 中文名稱：
    人酪蛋白α-S1(CSN1S1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL006060HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人酪蛋白α-S1(CSN1S1)酶聯(lián)免疫試劑盒（CSB-EL006060HU）為競爭法ELISA試劑盒，定量檢測breast牛奶樣本中的CSN1S1含量。CSN1S1是重要的靶點(diǎn)。它是乳蛋白基因，在乳腺合成酪蛋白過程中起關(guān)鍵作用。其研究機(jī)制主要圍繞對乳品質(zhì)影響展開，探究其基因表達(dá)調(diào)控影響酪蛋白合成量和結(jié)構(gòu)，進(jìn)而為改善乳品品質(zhì)、培育優(yōu)良乳用品種提供理論支撐。試劑盒檢測范圍為37.5 μg/mL-600 μg/mL，適用于乳制品基礎(chǔ)研究、母嬰營養(yǎng)學(xué)分析、嬰幼兒配方食品開發(fā)中的蛋白質(zhì)含量比對，以及乳源功能性成分的提取工藝評估等場景，為母乳成分?jǐn)?shù)據(jù)庫建立、哺乳期營養(yǎng)干預(yù)策略制定提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Alpha casein ELISA Kit; alpha-S1-casein ELISA Kit; CASA ELISA Kit; CASA1_HUMAN ELISA Kit; Casein alpha s1 ELISA Kit; casein; alpha ELISA Kit; casein; alpha-S1; included ELISA Kit; Casoxin-D ELISA Kit; CSN1 ELISA Kit; CSN1S1 ELISA Kit; Csna ELISA Kit; MGC149368 ELISA Kit
  • 縮寫：
    CSN1S1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    breast milk
  • 檢測范圍：
    37.5 μg/mL-600 μg/mL
  • 靈敏度：
    18.75 μg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CSN1S1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleBreast milk(n=4)
    1:10Average %90
    Range %86-97
    1:20Average %93
    Range %89-99
    1:40Average %95
    Range %90-107
    1:80Average %92
    Range %87-103
  • 回收率：
    The recovery of human CSN1S1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Breast milk (n=5) 9587-101
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/mlOD1OD2Average
    6000.215 0.233 0.224
    3000.349 0.374 0.362
    1500.655 0.612 0.634
    751.358 1.326 1.342
    37.51.764 1.727 1.746
    02.631 2.419 2.525
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Important role in the capacity of milk to transport calcium phosphate.; Casoxin D acts as opioid antagonist and has vasorelaxing activity mediated by bradykinin B1 receptors.
  • 基因功能參考文獻(xiàn)：
    1. Serine-71 in alpha-S1 casein is phosphorylated in the breast milk. PMID: 28786558
    2. Results suggest that autoimmunity to insulin can occur infrequently via cross-reactivity to food protein alpha casein. PMID: 21361910
    3. Casein alpha s1 is expressed in human monocytes and stimulates expression of proinflammatory cytokine granulocyte-macrophage colony-stimulating factor (GM-CSF) via an immunomodulatory role beyond the nutritional function of CSN1S1 in milk. PMID: 21135174
    4. alphas1-Casein may be a potential biomarker for early identification of BPH patients. PMID: 16683014
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Alpha-casein family
  • 組織特異性：
    Mammary gland specific. Secreted in milk.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 2445

    OMIM: 115450

    KEGG: hsa:1446

    STRING: 9606.ENSP00000246891

    UniGene: Hs.3155