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  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Human catestatin ELISA Kit
  • 中文名稱(chēng)：
    人兒茶酚抑素酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E17355h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人兒茶酚抑素酶聯(lián)免疫試劑盒（CSB-E17355h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、尿液樣本中的catestatin含量。人兒茶酚抑素（CST）是一種心血管活性多肽，其與慢性心力衰竭和室性心律失常存在密切聯(lián)系。研究表明，CST通過(guò)調(diào)節(jié)交感神經(jīng)活性、抑制炎癥反應(yīng)和促進(jìn)細(xì)胞凋亡等機(jī)制，在心血管系統(tǒng)中發(fā)揮保護(hù)作用，并可能成為治療相關(guān)疾病的新靶點(diǎn)。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，本產(chǎn)品可為神經(jīng)內(nèi)分泌調(diào)控機(jī)制、心血管疾病模型構(gòu)建、代謝綜合征相關(guān)研究提供可靠的實(shí)驗(yàn)工具。科研人員可通過(guò)探究不同生理或病理?xiàng)l件下（如高血壓、肥胖、糖尿病等）樣本中CST的濃度動(dòng)態(tài)變化，適用于細(xì)胞實(shí)驗(yàn)、動(dòng)物模型及臨床前研究的生物標(biāo)志物分析，但不用于臨床診斷。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    catestatin
  • 種屬：
    Homo sapiens (Human)
  • 樣本類(lèi)型：
    serum, plasma, cell culture supernates, urine
  • 檢測(cè)范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Biochemicals
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線(xiàn)性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human catestatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %101
    Range %97-105
    1:2Average %83
    Range %80-87
    1:4Average %92
    Range %87-94
    1:8Average %91
    Range %87-95
  • 回收率：
    The recovery of human catestatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9690-101
    EDTA plasma (n=4)9491-97
  • 標(biāo)準(zhǔn)曲線(xiàn)：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.564 2.438 2.501 2.317
    102.004 2.131 2.068 1.884
    51.533 1.568 1.551 1.367
    2.50.905 0.935 0.920 0.736
    1.250.618 0.608 0.613 0.429
    0.6250.432 0.445 0.439 0.255
    0.3120.297 0.287 0.292 0.108
    00.183 0.185 0.184
  • 本試劑盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human catestatin antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled catestatin antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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