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  4. Human chaperonin 10,CPN10 ELISA Kit

Human chaperonin 10,CPN10 ELISA Kit

  • 中文名稱(chēng)：
    人伴侶蛋白10(CPN10)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E09917h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人伴侶蛋白10(CPN10)酶聯(lián)免疫試劑盒（CSB-E09917h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的HSPE1含量。HSPE1是一個(gè)值得關(guān)注的靶點(diǎn)。它在細(xì)胞生理活動(dòng)中發(fā)揮重要作用，與細(xì)胞應(yīng)激反應(yīng)、蛋白質(zhì)折疊等相關(guān)。研究機(jī)制主要聚焦于其在疾病發(fā)生發(fā)展中的功能，如在腫瘤細(xì)胞增殖、存活等過(guò)程的作用，通過(guò)調(diào)控它或能找到新治療策略。試劑盒檢測(cè)范圍為0.16 ng/mL-10 ng/mL，適用于體外檢測(cè)CPN10在細(xì)胞應(yīng)激機(jī)制研究、疾病模型（如神經(jīng)退行性疾病或代謝綜合征）中的動(dòng)態(tài)表達(dá)分析，以及蛋白質(zhì)相互作用的基礎(chǔ)科研領(lǐng)域，為探索CPN10生物學(xué)功能提供可靠的實(shí)驗(yàn)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    10 kDa chaperonin ELISA Kit; 10 kDa heat shock protein mitochondrial ELISA Kit; 10 kDa heat shock protein; mitochondrial ELISA Kit; CH10_HUMAN ELISA Kit; Chaperonin 10 ELISA Kit; Chaperonin 10 homolog ELISA Kit; CPN10 ELISA Kit; cpn10 homolog ELISA Kit; Early pregnancy factor ELISA Kit; Early-pregnancy factor ELISA Kit; EPF ELISA Kit; GROES ELISA Kit; GroES homolog ELISA Kit; Heat shock 10kD protein 1 chaperonin 10 ELISA Kit; Heat shock 10kDa protein 1 ELISA Kit; Heat shock 10kDa protein 1 chaperonin 10 ELISA Kit; Heat shock protein family E (Hsp10) member ELISA Kit; Heat-shock 10-kD protein ELISA Kit; Hsp10 ELISA Kit; Hspe1 ELISA Kit
  • 縮寫(xiě)：
    HSPE1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.16 ng/mL-10 ng/mL
  • 靈敏度：
    0.04 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線(xiàn)性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CPN10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 92
    Range % 88-94
    1:2 Average % 102
    Range % 97-107
    1:4 Average % 94
    Range % 87-98
    1:8 Average % 102
    Range % 98-106
  • 回收率：
    The recovery of human CPN10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 83-94
    EDTA plasma (n=4) 92 87-96
  • 標(biāo)準(zhǔn)曲線(xiàn)：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.688 2.579 2.634 2.525
    5 2.154 2.054 2.104 1.995
    2.5 1.365 1.302 1.334 1.225
    1.25 0.742 0.734 0.738 0.629
    0.63 0.417 0.405 0.411 0.302
    0.31 0.274 0.266 0.270 0.161
    0.16 0.186 0.183 0.185 0.076
    0 0.110 0.108 0.109 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Co-chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp60, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein.
  • 基因功能參考文獻(xiàn)：
    1. elevated expression of HSP10 protein inhibits apoptosis and associates with poor prognosis of astrocytoma PMID: 29028811
    2. miR-146a, miR-146b, and miR-155 are exerting anti-inflammatory properties by down-regulating IL-6 and IL-8, and influencing the expression of HSP10 in the activated endothelium PMID: 28662100
    3. High expression of HSP10 is negatively associated with estrogen receptor/progesterone receptor status and might be a novel independent biomarker for poor prognosis in invasive ductal breast carcinoma. PMID: 27993580
    4. EPF induces the differentiation of regulatory T cells and increases their immunosuppressive activities. PMID: 27840373
    5. Cpn10 has a role in the spatial regulation of NPAT signaling PMID: 26429916
    6. Hsp10 has a role in nuclear localization and lung cells response to cigarette smoke PMID: 25355063
    7. Data show that that in presence of 300 mg/mL Ficoll the thermodynamic stability of each cpn10 monomer increases by over 30%, whereas the interfaces are stabilized by less than 10%. PMID: 21375247
    8. HSP10 protein was detected only in oocytes from human preantral follicles, whereas in antral follicles, it was localised in oocytes, granulosa cells, theca cells and stroma cells. PMID: 19903031
    9. Hereditary spastic paraplegia SPG13 is associated with a mutation in the gene encoding the mitochondrial chaperonin Hsp60. PMID: 11898127
    10. The low stability of the monomeric unit suggests that folding and assembly reactions for cpn10 are coupled. PMID: 12220543
    11. Cpn10 and placental lactogen are capable of stimulating the synthesis of type I collagen by human osteoblasts in culture PMID: 12703979
    12. Identification of amino acids important for the assembly of the cpn10 heptamer. PMID: 14525625
    13. complex mechanisms are involved in the protection by hsp10 against simulated ischemia and reoxygenation-induced myocyte death PMID: 15059967
    14. The HSP10 plays a role in bone marrow cell differentiation other than being a mitochondrial co-chaperonin. PMID: 15590416
    15. Chaperonin 10 and calgranulin A are identified as markers for diagnosis and screening of endometrial carcinoma. PMID: 15816004
    16. the cpn10 interfaces can adapt to structural alterations without loss of either subunit-subunit affinity or heptamer specificity PMID: 15978542
    17. biophysical analysis of dissociation equilibrium for the heptameric co-chaperonin proteins 10 from Aquifex aeolicus and human mitochondria PMID: 16100270
    18. proteomic analysis of possible role of heat shock protein 10 in colorectal cancer PMID: 16502466
    19. Results describe the time-resolved folding and assembly mechanism of the heptameric co-chaperonin protein 10 (cpn10) in vitro. PMID: 16979655
    20. Investigation of single-nucleotide variations in the Hsp10 gene and their disease-causing potential. PMID: 17072495
    21. In this review, we revise the involvement of Hsp10 in signal transduction pathways and its possible role in cancer etiology. PMID: 17278877
    22. Data show that Hhsp10 formed fibrils from only the acidic unfolded state and Core peptide regions of these protein fibrils were determined by proteolysis followed by a combination of Edman degradation and mass spectroscopy analyses. PMID: 18329043
    23. The effects of cpn 10 on cells of the oligodendrocyte lineage, were assessed. PMID: 18465204
    24. In patients with serous ovarian carcinomas, gene expression analysis coupled with immunohistochemistry allowed the identification of HSP10 as an independent factor of progression-free survival. PMID: 18500265
    25. Results describe a novel function of chaperonin 10 as a general differentiation factor, not limited to erythroid cells, and show how this biological effect is mediated by GSK-3alpha/beta. PMID: 19142874
    26. HSP10 was identified as a new autoantigen in both autoimmune pancreatitis and fulminant type 1 diabetes. PMID: 19520060

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  • 亞細(xì)胞定位：
    Mitochondrion matrix.
  • 蛋白家族：
    GroES chaperonin family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 5269

    OMIM: 600141

    KEGG: hsa:3336

    STRING: 9606.ENSP00000233893

    UniGene: Hs.1197