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  4. Human cleaved microtubule-associated protein tau (C-MAPT/C-TAU) ELISA kit

Human cleaved microtubule-associated protein tau (C-MAPT/C-TAU) ELISA kit

  • 中文名稱：
    人裂解微管相關(guān)蛋白tau(C-MAPT/C-TAU)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-ECL013481H
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人裂解微管相關(guān)蛋白tau(C-MAPT/C-TAU)酶聯(lián)免疫試劑盒（CSB-ECL013481H）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、腦脊液（CSF）、組織勻漿、細(xì)胞裂解物樣本中的C-MAPT/C-TAU含量。C-MAPT/C-TAU是研究tau蛋白的基因，tau蛋白是一種參與微管穩(wěn)定的微管相關(guān)蛋白。tau蛋白的異常磷酸化和聚集與阿爾茨海默病等神經(jīng)退行性疾病密切相關(guān)。研究C-MAPT/C-TAU有助于理解tau蛋白在神經(jīng)退行性疾病中的作用機(jī)制，為開發(fā)新的治療策略提供理論基礎(chǔ)。試劑盒檢測(cè)范圍為62.5 pg/mL-4000 pg/mL，適用于科研領(lǐng)域?qū)au蛋白的濃度分析；可用于評(píng)估疾病模型中的tau蛋白動(dòng)態(tài)變化、探究神經(jīng)退行機(jī)制及藥物干預(yù)效果研究，為神經(jīng)病理學(xué)、分子生物學(xué)實(shí)驗(yàn)提供可靠工具，適用于實(shí)驗(yàn)室基礎(chǔ)研究與轉(zhuǎn)化醫(yī)學(xué)探索本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    cleaved form of DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, TAU, G protein beta1/gamma2 subunit-interacting factor 1|microtubule-associated protein tau, isoform 4
  • 縮寫：
    C-MAPT/C-TAU
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cerebrospinal fluid (CSF), tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human C-MAPT/C-TAU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:5Average %102
    Range %96-106
    1:10Average %100
    Range %90-104
    1:20Average %95
    Range %85-99
    1:40Average %94
    Range %88-99
  • 回收率：
    The recovery of human C-MAPT/C-TAU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9082-95
    EDTA plasma (n=4)10091-104
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40003.001 2.889 2.945 2.832
    20002.114 1.976 2.045 1.932
    10001.231 1.200 1.216 1.103
    5000.596 0.565 0.581 0.468
    2500.353 0.341 0.347 0.234
    1250.239 0.232 0.236 0.123
    62.50.171 0.168 0.170 0.057
    00.111 0.115 0.113
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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