Human complement factor H,CFH ELISA Kit
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中文名稱:人補(bǔ)體因子H(CFH)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E08931h
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規(guī)格:96T/48T
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價(jià)格:¥3200/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人補(bǔ)體因子H(CFH)酶聯(lián)免疫試劑盒(CSB-E08931h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿.樣本中的CFH含量。CFH即補(bǔ)體因子H,是補(bǔ)體系統(tǒng)重要調(diào)節(jié)蛋白。其背景上,在維持補(bǔ)體激活和抑制平衡中起關(guān)鍵作用。研究機(jī)制方面,主要是通過與補(bǔ)體C3b結(jié)合,抑制補(bǔ)體旁路途徑過度激活,異常時(shí)可引發(fā)多種疾病,如年齡相關(guān)性黃斑變性等。試劑盒檢測范圍為31.2 ng/ml- 2000 ng/ml,靈敏度為7.8 ng/ml。適用于基礎(chǔ)科研領(lǐng)域,如研究補(bǔ)體系統(tǒng)在自身免疫性疾病、腎臟病理或炎癥反應(yīng)中的作用,或用于評估疾病模型動(dòng)物或體外實(shí)驗(yàn)中CFH的表達(dá)水平變化。其寬泛的檢測范圍可滿足不同濃度樣本的檢測需求,支持多樣本類型兼容性,為探索補(bǔ)體相關(guān)分子機(jī)制提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:adrenomedullin binding protein ELISA Kit; age related maculopathy susceptibility 1 ELISA Kit; AHUS 1 ELISA Kit; AHUS1 ELISA Kit; AMBP 1 ELISA Kit; AMBP1 ELISA Kit; ARMD 4 ELISA Kit; ARMD4 ELISA Kit; ARMS 1 ELISA Kit; ARMS1 ELISA Kit; beta 1 H globulin ELISA Kit; beta 1H ELISA Kit; beta1H ELISA Kit; CFAH_HUMAN ELISA Kit; CFH ELISA Kit; CFHL 3 ELISA Kit; CFHL3 ELISA Kit; Complement factor H ELISA Kit; complement factor H; isoform b ELISA Kit; Factor H ELISA Kit; factor H like 1 ELISA Kit; FH ELISA Kit; FHL 1 ELISA Kit; FHL1 ELISA Kit; H factor 1 (complement) ELISA Kit; H factor 1 ELISA Kit; H factor 2 (complement) ELISA Kit; HF 1 ELISA Kit; HF 2 ELISA Kit; HF ELISA Kit; HF1 ELISA Kit; HF2 ELISA Kit; HUS ELISA Kit; MGC88246 ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:31.2 ng/mL-2000 ng/mL
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靈敏度:7.8 ng/ml
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Immunology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(ng/ml)
245.723
250.139
256.612
242.927
251.751
258.647
SD
0.031
0.042
0.051
0.042
0.054
0.060
CV(%)
3.713
4.965
5.916
5.072
6.353
6.920
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human CFH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:200
Average %
89
Range %
80-94
1:400
Average %
97
Range %
91-102
1:800
Average %
101
Range %
94-107
1:1600
Average %
93
Range %
86-99
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回收率:
The recovery of human CFH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
95
89-99
EDTA plasma (n=4)
102
96-105
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
2000
2.566
2.466
2.516
2.424
1000
2.031
1.931
1.981
1.889
500
1.409
1.298
1.354
1.262
250
0.867
0.812
0.840
0.748
125
0.524
0.514
0.519
0.427
62.5
0.345
0.335
0.340
0.248
31.2
0.213
0.203
0.208
0.116
0
0.094
0.090
0.092
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Intercellular mitochondrial transfer as a means of revitalizing injured glomerular endothelial cells LX Tang,World Journal of Stem Cells,2022
- Complement Factor H and Related Proteins as Markers of Cardiovascular Risk in Pediatric Chronic Kidney Disease Wei-Ting Liao,Pharmaceuticals,2022
- Relationship between advanced glycated end products and maternal cognition in gestational diabetes: a case control study AK Sharma,The Journal of Maternal-Fetal & Neonatal Medicine,2021
- iTRAQ-based quantitative proteomic analysis reveals potential early diagnostic markers in serum of acute cellular rejection after liver transplantation Jiang Q, et al,Transplant immunology,2018
- Identification of Differentially Expressed Proteins in the Serum of Colorectal Cancer Patients Using 2D-DIGE Proteomics Analysis Lay Cheng Lim,.et al,Pathology & Oncology Research,2016
- Gelsolin and Ceruloplasmin as Potential Predictive Biomarkers for Cervical Cancer by 2D-DIGE Proteomics Analysis Lokamani I et al,Pathol Oncol Res,2013
- Serum proteomics of methamphetamine addicts and up-regulation of complement factor H related to methamphetamine addiction /,Neuroscience Letters,2012
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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最新研究進(jìn)展:CFH(Complement factor H)是補(bǔ)體系統(tǒng)中的一種血清蛋白,主要參與調(diào)節(jié)補(bǔ)體途徑的激活和炎癥反應(yīng)。最新研究表明,CFH在多種疾病中都起到了重要作用,如糖尿病、白內(nèi)障、自身免疫疾病等。CFH突變可導(dǎo)致眼部疾病和腎臟疾病,而CFH的過表達(dá)則與許多疾病,如肝硬化、慢性腎病和心臟病等相關(guān)。
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功能:Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces. Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop. As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b. In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed.
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基因功能參考文獻(xiàn):
- factors other than the CFH Y402H polymorphism may be involved in the progression of neovascular age-related macular degeneration after treatment with anti-VEGF agents, in Malaysian population PMID: 29579408
- Functional characterization of disease-associated genetic variants in the complement factor H gene in atypical hemolytic uremic syndrome and C3-glomerulopathy patients. PMID: 28941939
- Evasion of C3b deposition at division septa and lateral amplification underneath the capsule requires localization of the FH-binding protein PspC at division sites. PMID: 30139996
- These findings reveal that the CRP- and PTX3-binding characteristics of FHL-1 differ from those of FH, likely underpinning independent immune regulatory functions in the context of the human retina. PMID: 29374201
- Two rare age-related macular degeneration-associated variants in the CFH gene (rs121913059 [p.Arg1210Cys] and rs35292876) deviate in frequency among different geographic regions. PMID: 29410599
- these data demonstrate that Mesenchymal Stem Cells inhibit the activation of pathogenic C5 via up-regulation of FH, which improves our understanding of the immunomodulatory mechanisms of MSCs in the treatment of lupus nephritis. PMID: 29885865
- Mutation in CFH gene is associated with age-related macular degeneration. PMID: 29686068
- antioxidant and zinc nutritional supplement modifies risk of macular degeneration progression according to genotype PMID: 29311295
- CC rs1061170 CFH genotype may be associated with the age-related macular degeneration. Additionally, CC rs1061170 CFH genotype may promote a negative response to anti-VEGF treatment, while patients with TT rs1061170 CFH genotype showed better functional and structural response to anti-VEGF agents. PMID: 29912491
- Our analysis showed stronger contribution of ARMS2 in age-related macular degeneration (AMD) with reticular pseudodrusen (RPD) group versus AMD without RPD group, in comparison with CFH genotypes. PMID: 28593728
- in Chinese lupus nephritis patients, the variants in the FH gene might affect the histopathologic subtypes and some clinical features of the disease PMID: 28403670
- Study demonstrated that a novel complotype composed of CFB (rs4151667) in combination with CFB (rs641153) and CFH(rs800292) is strongly associated with complement activation and age-related macular degeneration status. PMID: 27241480
- The rs193053835 single nucleotide polymorphism showed the most significant protective effect to be associated with susceptibility to Meningococcal disease. PMID: 27805046
- Mapping rare, deleterious mutations in Factor H: Association with early onset, drusen burden, and lower antigenic levels in familial AMD. PMID: 27572114
- AMD patients had significantly elevated nitrated CFH levels compared to controls (p = 0.0117). These findings strongly suggest that nitrated CFH contributes to AMD progression, and is a target for therapeutic intervention. PMID: 28159936
- inhibition of the alternative pathway by factor H, with a concentration equivalent to a high physiological level, strongly reduced C5a levels and decreased proinflammatory cytokine production in human peripheral blood mononuclear cells. PMID: 27721145
- Complement factor H Y402H (rs1061170) and age-related maculopathy susceptibility2 (ARMS2)/LOC387715 A69S (rs10490924) polymorphisms shown to have significant association with age-related macular degeneration (Meta-Analysis). PMID: 27269047
- Regression analysis showed that ARMS2 TT genotype has a statistically significant effect on retinal angiomatous proliferation versus age-related macular degeneration compared to CFH genotypes (P < 0.001). PMID: 28005184
- This study enclosed strong synergistic association of risk genotypes of C3 and CFH Y402H with AMD. We also revealed synergistic influence of CCL2-2518 and the at-risk genotype of the C3 in AMD with an estimated AP = 50.9% (adjusted AP = 24.7%). Present findings show that CCL2-2518 polymorphism is not an innocent bystander in AMD susceptibility when combined with the at-risk genotype of C3 (R102G). PMID: 28095095
- Our results suggest that factor H can interfere with mycobacterial entry into macrophages and modulate inflammatory cytokine responses, particularly during the initial stages of infection, thus affecting the extracellular survival of the pathogen. PMID: 27262511
- To our knowledge, this is the first evaluation of the involvement of the CFHR3/CFHR1 deletion and age-related macular degeneration in CFH Y402H polymorphism Brazilian patients. PMID: 26942649
- The findings of the present study provide evidence that CFH gene variants and ARMS2/HTRA1 genes play a major role in the genetic susceptibility to AMD in a Greek population. These findings are of direct relevance for disease and help mapping the genetic chart of AMD. PMID: 26848857
- Our results suggest the contribution of all four predicted CFH polymorphisms in age-related macular degeneration (AMD) susceptibility among the Iranian population. This association with CFH may lead to early detection and new strategies for prevention and treatment of AMD. PMID: 25612476
- Development of polypoidal choroidal vasculopathy (PCV) in the unaffected fellow eye is associated with ARMS2 A69S genotype in patients with unilateral PCV. PMID: 26332911
- Identification of rare CFH variant carriers may be important for upcoming complement-inhibiting therapies. Patients with an extensive drusen area, drusen with crystalline appearance, and drusen nasal to the optic disc are more likely to have a rare variant in the CFH gene. PMID: 28859202
- C-reactive protein amino acids 35-47 mediate the interaction with complement factor H in lupus nephritis PMID: 28566480
- OCT scans revealed lower retinal thickness in patients homozygous for CFH or ARMS2, which was caused by a significantly reduced photoreceptor layer. The number and ultrastructure of drusen were also significantly different. PMID: 28558370
- VEGF inhibition decreases local CFH and other complement regulators in the eye and kidney through reduced VEGFR2/PKC-alpha/CREB signaling. PMID: 27918307
- CFH rs1061170 has an important effect on age at onset of MDD in Han Chinese and may therefore be related to early pathogenesis of MDD. PMID: 26941266
- Data suggest that disease-linked mutations in complement factor H (CFH) affect pivotal role of CFH in regulation of complement activation; mutations studied include those linked to atypical hemolytic uremic syndrome and age-related macular degeneration. PMID: 28637873
- Factor I binds C3b-Factor H between Factor H domains 2 and 3 and a reoriented C3b C-terminal domain and docks onto the first scissile bond, while stabilizing its catalytic domain for proteolytic activity. PMID: 28671664
- The VEGF haplotype TGA could be used as a marker for poor visual prognosis in Tunisian patients with neovascular AMD treated with bevacizumab. PMID: 27116510
- Two protective, low-frequency, non-synonymous variants were significantly associated with a decrease in age-related macular degeneration (AMD)risk: A307V in PELI3 and N1050Y in CFH .We also identified a strong protective signal for a common variant (rs8056814) near CTRB1 associated with a decrease in AMD risk (logistic regression: OR = 0.71, P = 1.8 x 10-07). PMID: 28011711
- this study shows that RNA interference of factor H in dendritic cells increased alloantigen-specific T-cell proliferation PMID: 28105653
- monomeric CRP (mCRP) but not the pentameric form (pCRP) upregulates IL-8 and CCL2 levels in retinal pigment epithelial cells. Complement factor H (FH) binds mCRP to dampen its proinflammatory activity. FH from AMD patients carrying the "risk" His402 polymorphism displays impaired binding to mCRP. PMID: 26961257
- The uromodulin-CFH interaction enhanced the cofactor activity of CFH for factor I-mediated cleavage of C3b to inactivated C3b. PMID: 27113631
- Our results revealed that SNPs CD59-rs831626 and CFH-rs1065489 were associated with the susceptibility of acute anterior uveitis. PMID: 27419833
- Our results show that age-related macular degeneration donors carrying the high risk allele for CFH (C) had significantly more mtDNA damage compared with donors having the wild-type genetic profile. PMID: 26854823
- Data suggest that Staphylococcus aureus surface protein SdrE (serine-aspartate repeat protein E) functions as a 'clamp' to capture C-terminal tail of human CFH (complement factor H) at a specific ligand-binding site/groove via unique close-dock-lock-latch mechanism and thereby sequesters CFH on surface of S. aureus for complement evasion (immune evasion). PMID: 28258151
- The haplotypic coinheritance of potentially functional variants (including missense variants, novel splice sites, and the CFHR3-CFHR1 deletion) was described for the four common haplotypes. Expression of the short and long CFH transcripts differed markedly between the retina and liver. PMID: 27196323
- Data suggest that complement factor H (CFH) attaches to surface of host cells to inhibit complement activation and amplification, preventing destruction of the host cells; SdrE (serine-aspartate repeat protein) of Staphylococcus aureus binds to CFH allowing S. aureus to mimic a host cell and reducing bacterial killing by granulocytes. [Commentary] PMID: 28490660
- Interaction effects between supplement groups and individual complement factor H (CFH) Y402H and age-related maculopathy susceptibility 2 (ARMS2) genotypes, and composite genetic risk groups combining the number of risk alleles for both loci, were evaluated for their association with progression PMID: 27471039
- EMD were not AMD-independently associated with CFH or ARMS2 genotypes. Our results indicate that patients without AMD but with EMD can serve as controls in studies evaluating AMD risk factors. PMID: 26614632
- Functional activities of FH are deficient in patients with ANCA-positive vasculitis. PMID: 27939215
- role for serum FH levels in the host response to invasive pneumococcal infections PMID: 26802141
- Despite limited power of this pilot study, our results suggest an association of HF with polymorphisms in ARMS2/HTRA1, CFH, APOE4/TOMM40, and VEGFA genes which could be triggered by modification of the extracellular matrix, altered complement system or lipid metabolism. PMID: 27552409
- data suggest that R1210C is a unique C-terminal complement factor H mutation that behaves as a partial complement factor H deficiency, predisposing individuals to diverse pathologies with distinct underlying pathogenic mechanisms; the final disease outcome is then determined by R1210C-independent genetic risk factors PMID: 26376859
- AMD progression rate is influenced by CFH, and suggest that variants within CFH may have different effects on risk versus progression. However, since CFH:rs10737680 was not significant after Bonferroni correction and explained only a relatively small portion of variation in progression rate beyond that explained by age PMID: 27832277
- We describe a novel CFH/CFHR3 hybrid gene secondary to a de novo 6.3-kb deletion that arose through microhomology-mediated end joining rather than nonallelic homologous recombination. We confirmed a transcript from this hybrid gene and showed a secreted protein product that lacks the recognition domain of factor H and exhibits impaired cell surface complement regulation PMID: 26490391
- The footprint of C3b on the FH surface matches existing crystal structures of C3b complexed with the N- and C-terminal fragments of FH. In addition, data revealed the position of the central portion of FH in the protein complex. Moreover, cross-linking studies confirmed the involvement of the C-terminus in the dimerization of FH. PMID: 27099340
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相關(guān)疾病:Basal laminar drusen (BLD); Complement factor H deficiency (CFHD); Hemolytic uremic syndrome atypical 1 (AHUS1); Macular degeneration, age-related, 4 (ARMD4)
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亞細(xì)胞定位:Secreted.
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組織特異性:Expressed in the retinal pigment epithelium (at protein level). CFH is one of the most abundant complement components in blood where the liver is the major source of CFH protein in vivo. in addition, CFH is secreted by additional cell types including mono
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