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  4. Human cytochrome c-1 (CYC1) ELISA kit

Human cytochrome c-1 (CYC1) ELISA kit

  • 中文名稱：
    人細(xì)胞色素C - 1(CYC1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E17854h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人細(xì)胞色素C - 1(CYC1)酶聯(lián)免疫試劑盒（CSB-E17854h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CYC1含量。CYC1是重要的細(xì)胞色素c1蛋白編碼基因，在細(xì)胞呼吸鏈電子傳遞中發(fā)揮關(guān)鍵作用。研究主要圍繞其在能量代謝中的功能機(jī)制，以及其異常與腫瘤、神經(jīng)退行性疾病等的關(guān)聯(lián)展開，以探尋新的疾病診斷標(biāo)志物和治療靶點。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于探索心血管疾病、神經(jīng)退行性疾病或癌癥模型中CYC1的生物學(xué)功能及動態(tài)變化，可為細(xì)胞代謝研究、藥物作用機(jī)制分析或疾病相關(guān)分子通路探索提供可靠數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Complex III subunit 4 ELISA Kit; Complex III subunit IV ELISA Kit; CY1_HUMAN ELISA Kit; CYC1 ELISA Kit; Cytochrome b c1 complex subunit 4 ELISA Kit; Cytochrome b-c1 complex subunit 4 ELISA Kit; Cytochrome bc1 complex subunit 4 ELISA Kit; Cytochrome c 1 ELISA Kit; Cytochrome c-1 ELISA Kit; Cytochrome c1; heme protein; mitochondrial ELISA Kit; Ubiquinol cytochrome c reductase complex cytochrome c1 subunit ELISA Kit; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit ELISA Kit; UQCR4 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of Human CYC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %89
    Range %85-93
    1:2Average %94
    Range %90-98
    1:4Average %92
    Range %88-96
    1:8Average %97
    Range %93-101
  • 回收率：
    The recovery of Human CYC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10297-106
    EDTA plasma (n=4)8783-91
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.863 2.967 2.915 2.817
    10002.526 2.425 2.476 2.378
    5001.875 1.776 1.826 1.728
    2501.065 1.043 1.054 0.956
    1250.547 0.524 0.536 0.438
    62.50.284 0.272 0.278 0.180
    31.250.198 0.189 0.194 0.096
    00.097 0.098 0.098
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. Cytochrome c1 is a catalytic core subunit containing a c-type heme. It transfers electrons from the [2Fe-2S] iron-sulfur cluster of the Rieske protein to cytochrome c.
  • 基因功能參考文獻(xiàn)：
    1. these results indicate that CYC1 plays important roles in cell proliferation and comedo necrosis through the elevated oxidative phosphorylation activity in human ductal carcinoma in situ PMID: 28394473
    2. results indicate that miR-661 plays a tumor suppressor role in OS mediated by the downregulation of CYC1, suggesting a potential mechanism underlying cell death resistance in Osteosarcoma. PMID: 28391262
    3. CYC1 promoted tumor metastasis via suppressing activation of AMPK and contributed to tumor growth via facilitating production of ATP. PMID: 27239088
    4. FASL, granzyme B, and cytochrome c blood expression reflects breast cancer progression and response to therapy. (Review) PMID: 27117663
    5. our results provide evidence that CYC1 plays an important role in OS tumorigenesis, and modulation of CYC1 may be an effective strategy to potentiate OS to apoptotic induction by TRAIL. PMID: 25562155
    6. Mutations in CYC1 cause insulin-responsive hyperglycemia. PMID: 23910460
    7. HeLa cells expressing non-cleavable cytochrome c1 maintain mitochondrial morphology and functions. PMID: 21577235
    8. Decreased electron Transport Complex IV activity is associated with ulcerative colitis. PMID: 20440543
    9. Addition of H202 to tendon fibroblasts in vitro resulted in oxidative stress-induced apoptosis and the release of CYC1. PMID: 12788227
    10. the Ca(2+) spike, mitochondrial Bcl-2 presensitization, and subsequent Delta psi(m) transition, Bax translocation, and ROS generation are important upstream signals for cytochrome c release upon stimulation by a Bcl-2 inhibitor. PMID: 14966123
    11. Sensitivity of Cyt c detection in -linked immunosorbent assay is reduced in the presence of serum leucine-rich alpha-2-glycoprotein-1. PMID: 16699948

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  • 相關(guān)疾病：
    Mitochondrial complex III deficiency, nuclear 6 (MC3DN6)
  • 亞細(xì)胞定位：
    Mitochondrion inner membrane; Single-pass membrane protein.
  • 蛋白家族：
    Cytochrome c family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 2579

    OMIM: 123980

    KEGG: hsa:1537

    STRING: 9606.ENSP00000317159

    UniGene: Hs.289271