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Human deoxypyridinoline,DPD ELISA Kit

  • 中文名稱:
    人脫氧吡啶酚/脫氧吡啶啉(DPD)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E08399h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人脫氧吡啶酚/脫氧吡啶啉(DPD)酶聯(lián)免疫試劑盒(CSB-E08399h)為雙抗夾心法ELISA試劑盒,定量檢測血清、尿液、組織培養(yǎng)上清液、組織勻漿樣本中的DPD含量。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL,可滿足骨代謝疾病機(jī)制研究、骨質(zhì)疏松模型評(píng)估、骨折愈合監(jiān)測或腫瘤骨轉(zhuǎn)移實(shí)驗(yàn)等科研需求;適用于實(shí)驗(yàn)室常規(guī)骨代謝相關(guān)生物標(biāo)志物的定量檢測。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    N/A
  • 縮寫:
    DPD
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, urine, cell culture supernates, tissue homogenates
  • 檢測范圍:
    1.56 ng/mL-100 ng/mL
  • 靈敏度:
    0.39 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human DPD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %88
    Range %80-92
    1:2Average %94
    Range %90-100
    1:4Average %96
    Range %91-106
    1:8Average %92
    Range %80-98
  • 回收率:
    The recovery of human DPD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-100
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.116 2.093 2.105 1.955
    501.912 1.803 1.858 1.708
    251.663 1.574 1.619 1.469
    12.51.258 1.186 1.222 1.072
    6.250.894 0.867 0.881 0.731
    3.120.521 0.502 0.512 0.362
    1.560.314 0.289 0.302 0.152
    00.152 0.148 0.150  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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