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  4. Human dermatopontin,DPT ELISA Kit

Human dermatopontin,DPT ELISA Kit

  • 中文名稱：
    人皮膚蛋白(DPT)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E11277h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人皮膚蛋白(DPT)酶聯(lián)免疫試劑盒（CSB-E11277h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的DPT含量。人皮膚蛋白DPT作為一種重要的細(xì)胞外基質(zhì)蛋白，其研究機(jī)制主要涉及其在皮膚纖維化、細(xì)胞粘附和信號傳導(dǎo)中的作用。DPT通過整合素與細(xì)胞表面結(jié)合，促進(jìn)細(xì)胞粘附，增強(qiáng)TGFβ1活性，抑制細(xì)胞增殖，促進(jìn)膠原纖維形成，并在低溫下穩(wěn)定膠原纖維。此外，DPT還與皮膚纖維化、創(chuàng)面愈合和衰老皮膚相關(guān)。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，支持科研人員探究皮膚疾病模型（如瘢痕形成、硬皮病）中DPT的動(dòng)態(tài)變化，評估藥物干預(yù)對皮膚纖維化通路的影響，或分析體外培養(yǎng)的成纖維細(xì)胞等模型中DPT的分泌水平。適用于基礎(chǔ)醫(yī)學(xué)、藥理學(xué)及皮膚生物學(xué)領(lǐng)域探索DPT在組織再生、細(xì)胞外基質(zhì)重塑等方向的功能機(jī)制，為相關(guān)疾病靶點(diǎn)發(fā)現(xiàn)及治療策略開發(fā)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    DERM_HUMAN ELISA Kit; Dermatopontin ELISA Kit; Dpt ELISA Kit; TRAMP ELISA Kit; Tyrosine rich acidic matrix protein ELISA Kit; Tyrosine-rich acidic matrix protein ELISA Kit
  • 縮寫：
    DPT
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human DPT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %85-102
    1:2Average %91
    Range %82-103
    1:4Average %95
    Range %86-108
    1:8Average %94
    Range %87-105
  • 回收率：
    The recovery of human DPT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9583-104
    EDTA plasma (n=4)9587-105
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.417 2.493 2.455 2.314
    101.763 1.817 1.790 1.649
    51.025 0.982 1.004 0.863
    2.50.611 0.595 0.603 0.462
    1.250.436 0.416 0.426 0.285
    0.6250.329 0.307 0.318 0.177
    0.3120.238 0.228 0.233 0.092
    00.142 0.139 0.141
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation.
  • 基因功能參考文獻(xiàn)：
    1. DPT gene silencing could effectively reduce the proliferation of MG-63 cells, slow down the cell cycle progression and promote cell apoptosis. PMID: 29115446
    2. Novel mutation in DPT segregates with the complete phenotype with variable expressivity in two pedigrees with autosomal dominant familial Meniere's disease. PMID: 27876815
    3. This study essentially deals with assessing the expression and integrity of an extracellular matrix protein, Dermatopontin (DPT), in chronic cutaneous wounds. PMID: 25260909
    4. dermatopontin functional peptide produces fibrinogen fibrils and modifies its biological activity PMID: 25082449
    5. These results indicate that DPT can modify certain biological functions of fibrin, and thus a another function of this extracellular matrix protein was revealed. PMID: 23877568
    6. data provided strong evidence that downregulation of DPT is a characteristic event in human oral squamous cell carcinoma and that DPT was correlated with cellular adhesion and invasiveness. PMID: 21796630
    7. DPT has an accelerating role in fibroblast cell adhesion to the provisional matrix in the initial stage of wound healing. PMID: 21398523
    8. suggests that dermatopontin can play various roles in different tissues and might be a molecule related to carcinogenesis and the progression of HCC via possible interaction with TGF-beta1 and other potential mechanisms PMID: 19916908
    9. roles of dermatopontin and histamine receptor H1 genes as downstream targets for the VDR were confirmed by gel electromotility shift and chromatin immunoprecipitation assays that showed the presence of VDR complex binding sequences PMID: 17547532

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  • 亞細(xì)胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Dermatopontin family
  • 組織特異性：
    Expressed in fibroblasts, heart, skeletal muscle, brain and pancreas. Expressed at an intermediate level in lung and kidney, and at a low level in liver and placenta. Expressed at a lower level in fibroblasts from hypertrophic scar lesional skin and in fi
  • 數(shù)據(jù)庫鏈接：

    HGNC: 3011

    OMIM: 125597

    KEGG: hsa:1805

    STRING: 9606.ENSP00000356791

    UniGene: Hs.80552